Decrease in nuclear phospholipids associated with DNA replication

Nadir M. Maraldi, Spartaco Santi, Nicoletta Zini, Andrea Ognibene, Riccardo Rizzoli, Giovanni Mazzotti, Roberto Di Primio, Renato Bareggi, Valeria Bertagnolo, Carla Pagliarini, Silvano Capitani

Research output: Contribution to journalArticlepeer-review


Lipid metabolism in nuclei is very active and appears involved in the transduction of signals to the genome in response to agonists acting at the plasma membrane level. However, the precise topology of nuclear lipid metabolism and the relationship between nuclear lipids and crucial events of the cell function, such as DNA replication, have not been fully elucidated. By using a recently developed cytochemical method for detecting phospholipids inside the nucleus of intact cells at the electron microscope level, we have analyzed the changes in intranuclear phospholipids in DNA-replicating versus resting cells, which are both present in the same sample of regenerating liver after partial hepatectomy. The pattern of DNA synthesis in replicating cells has been monitored by electron microscope immunocytochemistry after bromodeoxyuridine (BrdU) labeling. The data obtained, which allow a fine localization and a quantitative analysis of both DNA synthesis and phospholipid distribution, indicate a significant reduction in the phospholipids detectable inside the nucleus in all steps of the S phase. This could depend on an increased nuclear phospholipid hydrolysis, whose products should in turn activate some of the enzymes involved in the control of DNA replication.

Original languageEnglish
Pages (from-to)853-859
Number of pages7
JournalJournal of Cell Science
Issue number3
Publication statusPublished - Mar 1993


  • Bromodeoxyuridine
  • Electron microscopy
  • Immunocytochemistry
  • Nuclear phospholipids
  • S phase

ASJC Scopus subject areas

  • Cell Biology


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