Decrease in nuclear phospholipids associated with DNA replication

Nadir M. Maraldi, Spartaco Santi, Nicoletta Zini, Andrea Ognibene, Riccardo Rizzoli, Giovanni Mazzotti, Roberto Di Primio, Renato Bareggi, Valeria Bertagnolo, Carla Pagliarini, Silvano Capitani

Research output: Contribution to journalArticle

Abstract

Lipid metabolism in nuclei is very active and appears involved in the transduction of signals to the genome in response to agonists acting at the plasma membrane level. However, the precise topology of nuclear lipid metabolism and the relationship between nuclear lipids and crucial events of the cell function, such as DNA replication, have not been fully elucidated. By using a recently developed cytochemical method for detecting phospholipids inside the nucleus of intact cells at the electron microscope level, we have analyzed the changes in intranuclear phospholipids in DNA-replicating versus resting cells, which are both present in the same sample of regenerating liver after partial hepatectomy. The pattern of DNA synthesis in replicating cells has been monitored by electron microscope immunocytochemistry after bromodeoxyuridine (BrdU) labeling. The data obtained, which allow a fine localization and a quantitative analysis of both DNA synthesis and phospholipid distribution, indicate a significant reduction in the phospholipids detectable inside the nucleus in all steps of the S phase. This could depend on an increased nuclear phospholipid hydrolysis, whose products should in turn activate some of the enzymes involved in the control of DNA replication.

Original languageEnglish
Pages (from-to)853-859
Number of pages7
JournalJournal of Cell Science
Volume104
Issue number3
Publication statusPublished - Mar 1993

Fingerprint

DNA Replication
Phospholipids
Lipid Metabolism
DNA
Electrons
Hepatectomy
Bromodeoxyuridine
Cell Nucleus
S Phase
Signal Transduction
Hydrolysis
Immunohistochemistry
Cell Membrane
Genome
Lipids
Liver
Enzymes

Keywords

  • Bromodeoxyuridine
  • Electron microscopy
  • Immunocytochemistry
  • Nuclear phospholipids
  • S phase

ASJC Scopus subject areas

  • Cell Biology

Cite this

Maraldi, N. M., Santi, S., Zini, N., Ognibene, A., Rizzoli, R., Mazzotti, G., ... Capitani, S. (1993). Decrease in nuclear phospholipids associated with DNA replication. Journal of Cell Science, 104(3), 853-859.

Decrease in nuclear phospholipids associated with DNA replication. / Maraldi, Nadir M.; Santi, Spartaco; Zini, Nicoletta; Ognibene, Andrea; Rizzoli, Riccardo; Mazzotti, Giovanni; Di Primio, Roberto; Bareggi, Renato; Bertagnolo, Valeria; Pagliarini, Carla; Capitani, Silvano.

In: Journal of Cell Science, Vol. 104, No. 3, 03.1993, p. 853-859.

Research output: Contribution to journalArticle

Maraldi, NM, Santi, S, Zini, N, Ognibene, A, Rizzoli, R, Mazzotti, G, Di Primio, R, Bareggi, R, Bertagnolo, V, Pagliarini, C & Capitani, S 1993, 'Decrease in nuclear phospholipids associated with DNA replication', Journal of Cell Science, vol. 104, no. 3, pp. 853-859.
Maraldi NM, Santi S, Zini N, Ognibene A, Rizzoli R, Mazzotti G et al. Decrease in nuclear phospholipids associated with DNA replication. Journal of Cell Science. 1993 Mar;104(3):853-859.
Maraldi, Nadir M. ; Santi, Spartaco ; Zini, Nicoletta ; Ognibene, Andrea ; Rizzoli, Riccardo ; Mazzotti, Giovanni ; Di Primio, Roberto ; Bareggi, Renato ; Bertagnolo, Valeria ; Pagliarini, Carla ; Capitani, Silvano. / Decrease in nuclear phospholipids associated with DNA replication. In: Journal of Cell Science. 1993 ; Vol. 104, No. 3. pp. 853-859.
@article{c5330aef7b574598b1d0478fe0fcdf65,
title = "Decrease in nuclear phospholipids associated with DNA replication",
abstract = "Lipid metabolism in nuclei is very active and appears involved in the transduction of signals to the genome in response to agonists acting at the plasma membrane level. However, the precise topology of nuclear lipid metabolism and the relationship between nuclear lipids and crucial events of the cell function, such as DNA replication, have not been fully elucidated. By using a recently developed cytochemical method for detecting phospholipids inside the nucleus of intact cells at the electron microscope level, we have analyzed the changes in intranuclear phospholipids in DNA-replicating versus resting cells, which are both present in the same sample of regenerating liver after partial hepatectomy. The pattern of DNA synthesis in replicating cells has been monitored by electron microscope immunocytochemistry after bromodeoxyuridine (BrdU) labeling. The data obtained, which allow a fine localization and a quantitative analysis of both DNA synthesis and phospholipid distribution, indicate a significant reduction in the phospholipids detectable inside the nucleus in all steps of the S phase. This could depend on an increased nuclear phospholipid hydrolysis, whose products should in turn activate some of the enzymes involved in the control of DNA replication.",
keywords = "Bromodeoxyuridine, Electron microscopy, Immunocytochemistry, Nuclear phospholipids, S phase",
author = "Maraldi, {Nadir M.} and Spartaco Santi and Nicoletta Zini and Andrea Ognibene and Riccardo Rizzoli and Giovanni Mazzotti and {Di Primio}, Roberto and Renato Bareggi and Valeria Bertagnolo and Carla Pagliarini and Silvano Capitani",
year = "1993",
month = "3",
language = "English",
volume = "104",
pages = "853--859",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",
number = "3",

}

TY - JOUR

T1 - Decrease in nuclear phospholipids associated with DNA replication

AU - Maraldi, Nadir M.

AU - Santi, Spartaco

AU - Zini, Nicoletta

AU - Ognibene, Andrea

AU - Rizzoli, Riccardo

AU - Mazzotti, Giovanni

AU - Di Primio, Roberto

AU - Bareggi, Renato

AU - Bertagnolo, Valeria

AU - Pagliarini, Carla

AU - Capitani, Silvano

PY - 1993/3

Y1 - 1993/3

N2 - Lipid metabolism in nuclei is very active and appears involved in the transduction of signals to the genome in response to agonists acting at the plasma membrane level. However, the precise topology of nuclear lipid metabolism and the relationship between nuclear lipids and crucial events of the cell function, such as DNA replication, have not been fully elucidated. By using a recently developed cytochemical method for detecting phospholipids inside the nucleus of intact cells at the electron microscope level, we have analyzed the changes in intranuclear phospholipids in DNA-replicating versus resting cells, which are both present in the same sample of regenerating liver after partial hepatectomy. The pattern of DNA synthesis in replicating cells has been monitored by electron microscope immunocytochemistry after bromodeoxyuridine (BrdU) labeling. The data obtained, which allow a fine localization and a quantitative analysis of both DNA synthesis and phospholipid distribution, indicate a significant reduction in the phospholipids detectable inside the nucleus in all steps of the S phase. This could depend on an increased nuclear phospholipid hydrolysis, whose products should in turn activate some of the enzymes involved in the control of DNA replication.

AB - Lipid metabolism in nuclei is very active and appears involved in the transduction of signals to the genome in response to agonists acting at the plasma membrane level. However, the precise topology of nuclear lipid metabolism and the relationship between nuclear lipids and crucial events of the cell function, such as DNA replication, have not been fully elucidated. By using a recently developed cytochemical method for detecting phospholipids inside the nucleus of intact cells at the electron microscope level, we have analyzed the changes in intranuclear phospholipids in DNA-replicating versus resting cells, which are both present in the same sample of regenerating liver after partial hepatectomy. The pattern of DNA synthesis in replicating cells has been monitored by electron microscope immunocytochemistry after bromodeoxyuridine (BrdU) labeling. The data obtained, which allow a fine localization and a quantitative analysis of both DNA synthesis and phospholipid distribution, indicate a significant reduction in the phospholipids detectable inside the nucleus in all steps of the S phase. This could depend on an increased nuclear phospholipid hydrolysis, whose products should in turn activate some of the enzymes involved in the control of DNA replication.

KW - Bromodeoxyuridine

KW - Electron microscopy

KW - Immunocytochemistry

KW - Nuclear phospholipids

KW - S phase

UR - http://www.scopus.com/inward/record.url?scp=0027151810&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027151810&partnerID=8YFLogxK

M3 - Article

C2 - 8314878

AN - SCOPUS:0027151810

VL - 104

SP - 853

EP - 859

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - 3

ER -