Decrease in pathology and progression of scrapie after immunisation with synthetic prion protein peptides in hamsters

Giuliana Magri, Mario Clerici, Paola Dall'Ara, Mara Biasin, Maria Caramelli, Cristina Casalone, Maria Laura Giannino, Renato Longhi, Luca Piacentini, Silvia Della Bella, Paola Gazzuola, Piera Anna Martino, Silvia Della Bella, Claudia Pollera, Maria Puricelli, Francesco Servida, Ines Crescio, Adriano Boasso, Wilma Ponti, Giorgio Poli

Research output: Contribution to journalArticle

Abstract

Effective therapy for prion diseases is currently unavailable. Recently, vaccination was shown to be effective in mouse models of a particular neurodegenerative conditions: Alzheimer's disease (AD). Here, we report that vaccination with synthetic oligopeptides homologous to the hamster (Mesocricetus auratus) prion protein augments survival time in animals infected intraperitoneally with 263K scrapie agent. For each hamster included in the study, prion-specific serum antibodies as well as deposition of pathological prion protein (PrPres), glial fibrillary acidic protein (GFAP), and mRNA expression for cytokines (TNFα, IL-1β, IL-10) in brain tissues were evaluated. In immunized animals, increased survival after challenge was associated with a reduction of cerebral lesion, PrP deposition and GFAP expression; in these animals, anti-prion protein peptide antibody levels were increased, and the expression of pro-inflammatory cytokines (TNFα and IL-1β) was reduced. Vaccination could be an effective therapeutic approach to postpone disease onset.

Original languageEnglish
Pages (from-to)2862-2868
Number of pages7
JournalVaccine
Volume23
Issue number22
DOIs
Publication statusPublished - Apr 22 2005

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Keywords

  • Inflammation
  • Prion
  • Vaccination

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Magri, G., Clerici, M., Dall'Ara, P., Biasin, M., Caramelli, M., Casalone, C., Giannino, M. L., Longhi, R., Piacentini, L., Della Bella, S., Gazzuola, P., Martino, P. A., Della Bella, S., Pollera, C., Puricelli, M., Servida, F., Crescio, I., Boasso, A., Ponti, W., & Poli, G. (2005). Decrease in pathology and progression of scrapie after immunisation with synthetic prion protein peptides in hamsters. Vaccine, 23(22), 2862-2868. https://doi.org/10.1016/j.vaccine.2004.11.067