Decreased contractile responses to isoproterenol in isolated cardiac myocytes from ageing guinea-pigs

Nicola Ferrara, Peter O'Gara, Dylan G. Wynne, Lesley A. Brown, Federica del Monte, Philip A. Poole-Wilson, Sian E. Harding

Research output: Contribution to journalArticle

Abstract

We have characterized the age-related changes of contractility and β-adrenoceptor function in isolated cardiac myocytes from guinea-pigs. We used either adult animals from 2 to 14 weeks of age, where body weight increases linearly with age, or senescent ones aged between 53-65 weeks. There was some indication of a decrease in contractility in maximum Ca2+ with age, with significant differences between a young (≤4 weeks, weight 600 g) group in contraction amplitude expressed as percentage shortening (but not when expressed as μm change in length) or contraction and relaxation velocities. This decline was continued into senescence, and ANOVA showed a significant difference between the three groups for contraction amplitude (percentage shortening, 12.2 ± 0.9%, young, n = 31; 9.5 ± 0.6%, n = 28 aged; 6.7 ± 0.8%, n = 6, senescent; P = 0.005), and contraction or relaxation velocities (P50 (P2+ ratio (P2+, suggesting a specific lesion in the adenylate cyclase related pathway. Much of the change occurred between the young adult (≤4 weeks) and the aged adult (≥8 weeks), although the trend was continued in senescent animals (>52 weeks).

Original languageEnglish
Pages (from-to)1141-1150
Number of pages10
JournalJournal of Molecular and Cellular Cardiology
Volume27
Issue number5
Publication statusPublished - May 1995

Keywords

  • β-adrenoceptor
  • Aged heart
  • Calcium
  • Cardiomyocyte

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Molecular Biology

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  • Cite this

    Ferrara, N., O'Gara, P., Wynne, D. G., Brown, L. A., del Monte, F., Poole-Wilson, P. A., & Harding, S. E. (1995). Decreased contractile responses to isoproterenol in isolated cardiac myocytes from ageing guinea-pigs. Journal of Molecular and Cellular Cardiology, 27(5), 1141-1150.