Decreased muscle acetyl-coenzyme A carboxylase 2 mRNA and insulin resistance in formerly obese subjects

Giuseppina Rosa, Melania Manco, Natalie Vega, Aldo V. Greco, Marco Castagneto, Hubert Vidal, Geltrude Mingrone

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Objective: A relationship between free fatty acids, intramuscular triglycerides (TGMs), and insulin resistance is widely accepted. The intracellular level of malonyl-coenzyme A (CoA) was suggested to be the possible link. Acetyl-CoA carboxylase (ACC) is a key enzyme in fatty acid metabolism, catalyzing the synthesis of malonyl-CoA, a fatty acid acyl-chain elongation unit, from acetyl-CoA. We assessed ACC2 mRNA expression variations in skeletal muscle of subjects who have undergone biliopancreatic diversion (BPD) operation. BPD, in fact inducing a massive lipid malabsorption, leads to a reversion of insulin resistance. Research Methods and Procedures: Twelve obese women (BMI > 40 kg/m2) were enrolled in the study. Body composition, euglycemic-hyperinsulinemic clamp, and muscle biopsies for lipid analysis and reverse transcription-competitive polymerase chain reaction were performed before and 3 years after BPD. Results: The average weight loss was around 37%. A significant inverse linear relation was observed between glucose uptake and TGM (y = -5.62x - 142.82, R2 = 0.50, p = 0.01). The reduced amount of ACC2 mRNA directly correlated with both TGM (y = 2.11x +69.85, R2 = 0.70, p = 0.01) and fasting insulin (y = 1.49x + 57.17, R2 = 0.69, p <0.01) concentrations. Discussion: In conclusion, down-regulation of ACC2 mRNA, induced by the lowering of plasma insulin concentration, is related to improvement of insulin sensitivity. We hypothesize that reduced amount of malonyl-CoA, consequent to reduced ACC2 mRNA, enhancing fatty acid oxidation, causes lowering of the intramyocitic triglyceride depot.

Original languageEnglish
Pages (from-to)1306-1312
Number of pages7
JournalObesity Research
Volume11
Issue number11
Publication statusPublished - 2003

Fingerprint

malonyl coenzyme A
Acetyl-CoA Carboxylase
acetyl-CoA carboxylase
Biliopancreatic Diversion
Malonyl Coenzyme A
insulin resistance
Insulin Resistance
Muscles
muscles
Messenger RNA
Fatty Acids
insulin
acetyl coenzyme A
Triglycerides
reverse transcription
beta oxidation
fatty acid metabolism
malabsorption
intramuscular fat
Insulin

Keywords

  • Acetyl-coenzyme A carboxylase
  • Biliopancreatic diversion
  • Insulin resistance
  • Intramyocitic triglycerides

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Public Health, Environmental and Occupational Health
  • Endocrinology
  • Food Science
  • Endocrinology, Diabetes and Metabolism

Cite this

Rosa, G., Manco, M., Vega, N., Greco, A. V., Castagneto, M., Vidal, H., & Mingrone, G. (2003). Decreased muscle acetyl-coenzyme A carboxylase 2 mRNA and insulin resistance in formerly obese subjects. Obesity Research, 11(11), 1306-1312.

Decreased muscle acetyl-coenzyme A carboxylase 2 mRNA and insulin resistance in formerly obese subjects. / Rosa, Giuseppina; Manco, Melania; Vega, Natalie; Greco, Aldo V.; Castagneto, Marco; Vidal, Hubert; Mingrone, Geltrude.

In: Obesity Research, Vol. 11, No. 11, 2003, p. 1306-1312.

Research output: Contribution to journalArticle

Rosa, G, Manco, M, Vega, N, Greco, AV, Castagneto, M, Vidal, H & Mingrone, G 2003, 'Decreased muscle acetyl-coenzyme A carboxylase 2 mRNA and insulin resistance in formerly obese subjects', Obesity Research, vol. 11, no. 11, pp. 1306-1312.
Rosa, Giuseppina ; Manco, Melania ; Vega, Natalie ; Greco, Aldo V. ; Castagneto, Marco ; Vidal, Hubert ; Mingrone, Geltrude. / Decreased muscle acetyl-coenzyme A carboxylase 2 mRNA and insulin resistance in formerly obese subjects. In: Obesity Research. 2003 ; Vol. 11, No. 11. pp. 1306-1312.
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abstract = "Objective: A relationship between free fatty acids, intramuscular triglycerides (TGMs), and insulin resistance is widely accepted. The intracellular level of malonyl-coenzyme A (CoA) was suggested to be the possible link. Acetyl-CoA carboxylase (ACC) is a key enzyme in fatty acid metabolism, catalyzing the synthesis of malonyl-CoA, a fatty acid acyl-chain elongation unit, from acetyl-CoA. We assessed ACC2 mRNA expression variations in skeletal muscle of subjects who have undergone biliopancreatic diversion (BPD) operation. BPD, in fact inducing a massive lipid malabsorption, leads to a reversion of insulin resistance. Research Methods and Procedures: Twelve obese women (BMI > 40 kg/m2) were enrolled in the study. Body composition, euglycemic-hyperinsulinemic clamp, and muscle biopsies for lipid analysis and reverse transcription-competitive polymerase chain reaction were performed before and 3 years after BPD. Results: The average weight loss was around 37{\%}. A significant inverse linear relation was observed between glucose uptake and TGM (y = -5.62x - 142.82, R2 = 0.50, p = 0.01). The reduced amount of ACC2 mRNA directly correlated with both TGM (y = 2.11x +69.85, R2 = 0.70, p = 0.01) and fasting insulin (y = 1.49x + 57.17, R2 = 0.69, p <0.01) concentrations. Discussion: In conclusion, down-regulation of ACC2 mRNA, induced by the lowering of plasma insulin concentration, is related to improvement of insulin sensitivity. We hypothesize that reduced amount of malonyl-CoA, consequent to reduced ACC2 mRNA, enhancing fatty acid oxidation, causes lowering of the intramyocitic triglyceride depot.",
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AU - Vidal, Hubert

AU - Mingrone, Geltrude

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N2 - Objective: A relationship between free fatty acids, intramuscular triglycerides (TGMs), and insulin resistance is widely accepted. The intracellular level of malonyl-coenzyme A (CoA) was suggested to be the possible link. Acetyl-CoA carboxylase (ACC) is a key enzyme in fatty acid metabolism, catalyzing the synthesis of malonyl-CoA, a fatty acid acyl-chain elongation unit, from acetyl-CoA. We assessed ACC2 mRNA expression variations in skeletal muscle of subjects who have undergone biliopancreatic diversion (BPD) operation. BPD, in fact inducing a massive lipid malabsorption, leads to a reversion of insulin resistance. Research Methods and Procedures: Twelve obese women (BMI > 40 kg/m2) were enrolled in the study. Body composition, euglycemic-hyperinsulinemic clamp, and muscle biopsies for lipid analysis and reverse transcription-competitive polymerase chain reaction were performed before and 3 years after BPD. Results: The average weight loss was around 37%. A significant inverse linear relation was observed between glucose uptake and TGM (y = -5.62x - 142.82, R2 = 0.50, p = 0.01). The reduced amount of ACC2 mRNA directly correlated with both TGM (y = 2.11x +69.85, R2 = 0.70, p = 0.01) and fasting insulin (y = 1.49x + 57.17, R2 = 0.69, p <0.01) concentrations. Discussion: In conclusion, down-regulation of ACC2 mRNA, induced by the lowering of plasma insulin concentration, is related to improvement of insulin sensitivity. We hypothesize that reduced amount of malonyl-CoA, consequent to reduced ACC2 mRNA, enhancing fatty acid oxidation, causes lowering of the intramyocitic triglyceride depot.

AB - Objective: A relationship between free fatty acids, intramuscular triglycerides (TGMs), and insulin resistance is widely accepted. The intracellular level of malonyl-coenzyme A (CoA) was suggested to be the possible link. Acetyl-CoA carboxylase (ACC) is a key enzyme in fatty acid metabolism, catalyzing the synthesis of malonyl-CoA, a fatty acid acyl-chain elongation unit, from acetyl-CoA. We assessed ACC2 mRNA expression variations in skeletal muscle of subjects who have undergone biliopancreatic diversion (BPD) operation. BPD, in fact inducing a massive lipid malabsorption, leads to a reversion of insulin resistance. Research Methods and Procedures: Twelve obese women (BMI > 40 kg/m2) were enrolled in the study. Body composition, euglycemic-hyperinsulinemic clamp, and muscle biopsies for lipid analysis and reverse transcription-competitive polymerase chain reaction were performed before and 3 years after BPD. Results: The average weight loss was around 37%. A significant inverse linear relation was observed between glucose uptake and TGM (y = -5.62x - 142.82, R2 = 0.50, p = 0.01). The reduced amount of ACC2 mRNA directly correlated with both TGM (y = 2.11x +69.85, R2 = 0.70, p = 0.01) and fasting insulin (y = 1.49x + 57.17, R2 = 0.69, p <0.01) concentrations. Discussion: In conclusion, down-regulation of ACC2 mRNA, induced by the lowering of plasma insulin concentration, is related to improvement of insulin sensitivity. We hypothesize that reduced amount of malonyl-CoA, consequent to reduced ACC2 mRNA, enhancing fatty acid oxidation, causes lowering of the intramyocitic triglyceride depot.

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