Decreased presence of perforated synapses in a triple-transgenic mouse model of Alzheimer's disease

Carlo Bertoni-Freddari, Stefano L. Sensi, Belinda Giorgetti, Marta Balietti, Giuseppina Di Stefano, Lorella M T Canzoniero, Tiziana Casoli, Patrizia Fattoretti

Research output: Contribution to journalArticlepeer-review

Abstract

Transgenic mouse models of Alzheimer's disease (AD) are useful tools to further our understanding of AD genotype-phenotype interaction. The triple transgenic mice harboring mutant forms of APP/PS1/Tau (3xTg-AD) exhibit β-amyloid (Aβ) plaques (by 6 months of age) as well as neurofibrillary tangles (by 10-12 months of age). In this study, we characterized morphological alterations of hippocampal synapses obtained from 13-month-old 3xTg-AD and age-matched control (PS1-KI) mice. Numeric density of synapses (Nv, number of junctions/μm3 of tissue), average synaptic contact area (S), and synaptic surface density (Sv, total synaptic contact area/μm3 of tissue) were investigated by morphometric methods in the AD vulnerable CA1 pyramidal cell layer. Comparisons between 3xTg-AD and control mice showed no statistically significant differences in any of the three parameters; however, a significant decrease (by 28.5%) in the fraction of perforated junctional areas (PS) was observed in the 3xTg-AD mice. As PS is a reliably indirect index of synaptic plasticity, a decreased PS number might represent a subtle and early sign of synaptic dysfunction occurring in the 3xTg-AD mice, and lend support to the hypothesis that altered synaptic function is a critical feature of AD.

Original languageEnglish
Pages (from-to)309-313
Number of pages5
JournalRejuvenation Research
Volume11
Issue number2
DOIs
Publication statusPublished - Apr 1 2008

ASJC Scopus subject areas

  • Ageing

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