Abstract
Laser assisted keratoplasty is nowadays largely used to perform minimally invasive surgery and partial thickness keratoplasty [1-3]. The use of the femtosecond laser enables to perform a customized surgery, solving the specific problem of the single patient, designing new graft profiles and partial thickness keratoplasty (PTK). The common characteristics of the PTKs and that make them eligible respect to the standard penetrating keratoplasty, are: the preservation of eyeball integrity, a reduced risk of graft rejection, a controlled postoperative astigmatism. On the other hand, the optimal surgical results after these PTKs are related to a correct comprehension of the deep stroma layers morphology, which can help in the identification of the correct cleavage plane during surgeries. In the last years some studies were published, giving new insights about the posterior stroma morphology in adult subjects [4,5]. In this work we present a study performed on two groups of tissues: one group is from 20 adult subjects aged 59 ± 18 y.o., and the other group is from 15 young subjects, aged 12±5 y.o. The samples were from tissues not suitable for transplant in patients. Confocal microscopy and Environmental Scanning Electron Microscopy (ESEM) were used for the analysis of the deep stroma. The preliminary results of this analysis show the main differences in between young and adult tissues, enabling to improve the knowledge of the morphology and of the biomechanical properties of human cornea, in order to improve the surgical results in partial thickness keratoplasty.
| Original language | English |
|---|---|
| Title of host publication | Ophthalmic Technologies XXV |
| Publisher | SPIE |
| Volume | 9307 |
| ISBN (Print) | 9781628413977 |
| DOIs | |
| Publication status | Published - 2015 |
| Event | 25th Conference on Ophthalmic Technologies - San Francisco, United States Duration: Feb 7 2015 → Feb 8 2015 |
Other
| Other | 25th Conference on Ophthalmic Technologies |
|---|---|
| Country | United States |
| City | San Francisco |
| Period | 2/7/15 → 2/8/15 |
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Keywords
- anterior lamellar keratoplasty
- confocal microscopy
- deep stroma
- endothelial keratoplasty
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Radiology Nuclear Medicine and imaging
Cite this
Deep stroma investigation by confocal microscopy. / Rossi, Francesca; Tatini, Francesca; Pini, Roberto; Valente, Paola; Ardia, Roberta; Buzzonetti, Luca; Canovetti, Annalisa; Malandrini, Alex; Lenzetti, Ivo; Menabuoni, Luca.
Ophthalmic Technologies XXV. Vol. 9307 SPIE, 2015. 93071H.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Deep stroma investigation by confocal microscopy
AU - Rossi, Francesca
AU - Tatini, Francesca
AU - Pini, Roberto
AU - Valente, Paola
AU - Ardia, Roberta
AU - Buzzonetti, Luca
AU - Canovetti, Annalisa
AU - Malandrini, Alex
AU - Lenzetti, Ivo
AU - Menabuoni, Luca
PY - 2015
Y1 - 2015
N2 - Laser assisted keratoplasty is nowadays largely used to perform minimally invasive surgery and partial thickness keratoplasty [1-3]. The use of the femtosecond laser enables to perform a customized surgery, solving the specific problem of the single patient, designing new graft profiles and partial thickness keratoplasty (PTK). The common characteristics of the PTKs and that make them eligible respect to the standard penetrating keratoplasty, are: the preservation of eyeball integrity, a reduced risk of graft rejection, a controlled postoperative astigmatism. On the other hand, the optimal surgical results after these PTKs are related to a correct comprehension of the deep stroma layers morphology, which can help in the identification of the correct cleavage plane during surgeries. In the last years some studies were published, giving new insights about the posterior stroma morphology in adult subjects [4,5]. In this work we present a study performed on two groups of tissues: one group is from 20 adult subjects aged 59 ± 18 y.o., and the other group is from 15 young subjects, aged 12±5 y.o. The samples were from tissues not suitable for transplant in patients. Confocal microscopy and Environmental Scanning Electron Microscopy (ESEM) were used for the analysis of the deep stroma. The preliminary results of this analysis show the main differences in between young and adult tissues, enabling to improve the knowledge of the morphology and of the biomechanical properties of human cornea, in order to improve the surgical results in partial thickness keratoplasty.
AB - Laser assisted keratoplasty is nowadays largely used to perform minimally invasive surgery and partial thickness keratoplasty [1-3]. The use of the femtosecond laser enables to perform a customized surgery, solving the specific problem of the single patient, designing new graft profiles and partial thickness keratoplasty (PTK). The common characteristics of the PTKs and that make them eligible respect to the standard penetrating keratoplasty, are: the preservation of eyeball integrity, a reduced risk of graft rejection, a controlled postoperative astigmatism. On the other hand, the optimal surgical results after these PTKs are related to a correct comprehension of the deep stroma layers morphology, which can help in the identification of the correct cleavage plane during surgeries. In the last years some studies were published, giving new insights about the posterior stroma morphology in adult subjects [4,5]. In this work we present a study performed on two groups of tissues: one group is from 20 adult subjects aged 59 ± 18 y.o., and the other group is from 15 young subjects, aged 12±5 y.o. The samples were from tissues not suitable for transplant in patients. Confocal microscopy and Environmental Scanning Electron Microscopy (ESEM) were used for the analysis of the deep stroma. The preliminary results of this analysis show the main differences in between young and adult tissues, enabling to improve the knowledge of the morphology and of the biomechanical properties of human cornea, in order to improve the surgical results in partial thickness keratoplasty.
KW - anterior lamellar keratoplasty
KW - confocal microscopy
KW - deep stroma
KW - endothelial keratoplasty
UR - http://www.scopus.com/inward/record.url?scp=84928568128&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84928568128&partnerID=8YFLogxK
U2 - 10.1117/12.2077112
DO - 10.1117/12.2077112
M3 - Conference contribution
AN - SCOPUS:84928568128
SN - 9781628413977
VL - 9307
BT - Ophthalmic Technologies XXV
PB - SPIE
ER -