Deep subcutaneous adipose tissue: A distinct abdominal adipose depot

Gillian E. Walker, Barbara Verti, Paolo Marzullo, Guillo Savia, Monica Mencarelli, Francesco Zurleni, Antonio Liuzzi, Anna Maria Di Blasio

Research output: Contribution to journalArticle

Abstract

Objective: Abdominal visceral (VAT) and subcutaneous adipose tissue (SAT) display significam metabolic differences, with VAT showing a functional association to metabolic/cardiovascular disorders. A third abdominal adipose layer, derived by the division of SAT and identified as deep subcutaneous adipose tissue (dSAT), may play a significant and independent metabolic role. The aim of this study was to evaluate depot-specific differences in the expression of proteins key to adipocyte metabolism in a lean population to establish a potential physiologic role for dSAT. Research Methods and Procedures: Adipocytes and preadipocytes were isolated from whole biopsies taken from superficial SAT (sSAT), dSAT, and VAT samples obtained from 10 healthy normal weight patients (7 women and 3 men), with a mean age of 56.4 ± 4.04 years and a mean BMI of 23.1 ± 0.5 kg/m2. Samples were evaluated for depot-specific differences in insulin sensitivity using adiponectin, glucose transport protein 4 (GLUT4), and resistin mRNA and protein expression, glucocorticoid metabolism by 11β-hydroxysteroid dehydrogenase type-1 (11β-HSD1) expression, and alterations in the adipokines leptin and tumor necrosis factor-α (TNF-α). Results: Although no regional differences in expression were observed for adiponectin or TNF-α, dSAT whole biopsies and adipocytes, while intermediary to both sSAT and VAT, reflected more of the VAT expression profile of 11β-HSD1, leptin, and resistin. Only in the case of the intracellular pool of GLUT4 proteins in whole biopsies was an independent pattern of expression observed for dSAT. In an evaluation of the homeostatic model, dSAT 11β-HSD1 protein (r = 0.9573, p = 0.0002) and TNF-α mRNA (r = 0.8210, p = 0.0236) correlated positively to the homeostatic model. Discussion: Overall, dSAT seems to be a distinct abdominal adipose depot supporting an independent metabolic function that may have a potential role in the development of obesity-associated complications.

Original languageEnglish
Pages (from-to)1933-1943
Number of pages11
JournalObesity
Volume15
Issue number8
DOIs
Publication statusPublished - Aug 2007

Fingerprint

Subcutaneous Fat
11-beta-Hydroxysteroid Dehydrogenases
Adipocytes
Resistin
Facilitative Glucose Transport Proteins
Tumor Necrosis Factor-alpha
Adiponectin
Leptin
Biopsy
Proteins
Abdominal Fat
Messenger RNA
Adipokines
Intra-Abdominal Fat
Glucocorticoids
Insulin Resistance
Obesity
Weights and Measures

Keywords

  • Adipocytes
  • Deep subcutaneous
  • Preadipocytes
  • Superficial subcutaneous
  • Visceral

ASJC Scopus subject areas

  • Endocrinology
  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Deep subcutaneous adipose tissue : A distinct abdominal adipose depot. / Walker, Gillian E.; Verti, Barbara; Marzullo, Paolo; Savia, Guillo; Mencarelli, Monica; Zurleni, Francesco; Liuzzi, Antonio; Di Blasio, Anna Maria.

In: Obesity, Vol. 15, No. 8, 08.2007, p. 1933-1943.

Research output: Contribution to journalArticle

Walker, Gillian E. ; Verti, Barbara ; Marzullo, Paolo ; Savia, Guillo ; Mencarelli, Monica ; Zurleni, Francesco ; Liuzzi, Antonio ; Di Blasio, Anna Maria. / Deep subcutaneous adipose tissue : A distinct abdominal adipose depot. In: Obesity. 2007 ; Vol. 15, No. 8. pp. 1933-1943.
@article{f70658bf8c5b4e1a957d11f226743e47,
title = "Deep subcutaneous adipose tissue: A distinct abdominal adipose depot",
abstract = "Objective: Abdominal visceral (VAT) and subcutaneous adipose tissue (SAT) display significam metabolic differences, with VAT showing a functional association to metabolic/cardiovascular disorders. A third abdominal adipose layer, derived by the division of SAT and identified as deep subcutaneous adipose tissue (dSAT), may play a significant and independent metabolic role. The aim of this study was to evaluate depot-specific differences in the expression of proteins key to adipocyte metabolism in a lean population to establish a potential physiologic role for dSAT. Research Methods and Procedures: Adipocytes and preadipocytes were isolated from whole biopsies taken from superficial SAT (sSAT), dSAT, and VAT samples obtained from 10 healthy normal weight patients (7 women and 3 men), with a mean age of 56.4 ± 4.04 years and a mean BMI of 23.1 ± 0.5 kg/m2. Samples were evaluated for depot-specific differences in insulin sensitivity using adiponectin, glucose transport protein 4 (GLUT4), and resistin mRNA and protein expression, glucocorticoid metabolism by 11β-hydroxysteroid dehydrogenase type-1 (11β-HSD1) expression, and alterations in the adipokines leptin and tumor necrosis factor-α (TNF-α). Results: Although no regional differences in expression were observed for adiponectin or TNF-α, dSAT whole biopsies and adipocytes, while intermediary to both sSAT and VAT, reflected more of the VAT expression profile of 11β-HSD1, leptin, and resistin. Only in the case of the intracellular pool of GLUT4 proteins in whole biopsies was an independent pattern of expression observed for dSAT. In an evaluation of the homeostatic model, dSAT 11β-HSD1 protein (r = 0.9573, p = 0.0002) and TNF-α mRNA (r = 0.8210, p = 0.0236) correlated positively to the homeostatic model. Discussion: Overall, dSAT seems to be a distinct abdominal adipose depot supporting an independent metabolic function that may have a potential role in the development of obesity-associated complications.",
keywords = "Adipocytes, Deep subcutaneous, Preadipocytes, Superficial subcutaneous, Visceral",
author = "Walker, {Gillian E.} and Barbara Verti and Paolo Marzullo and Guillo Savia and Monica Mencarelli and Francesco Zurleni and Antonio Liuzzi and {Di Blasio}, {Anna Maria}",
year = "2007",
month = "8",
doi = "10.1038/oby.2007.231",
language = "English",
volume = "15",
pages = "1933--1943",
journal = "Obesity",
issn = "1930-7381",
publisher = "Wiley-Blackwell",
number = "8",

}

TY - JOUR

T1 - Deep subcutaneous adipose tissue

T2 - A distinct abdominal adipose depot

AU - Walker, Gillian E.

AU - Verti, Barbara

AU - Marzullo, Paolo

AU - Savia, Guillo

AU - Mencarelli, Monica

AU - Zurleni, Francesco

AU - Liuzzi, Antonio

AU - Di Blasio, Anna Maria

PY - 2007/8

Y1 - 2007/8

N2 - Objective: Abdominal visceral (VAT) and subcutaneous adipose tissue (SAT) display significam metabolic differences, with VAT showing a functional association to metabolic/cardiovascular disorders. A third abdominal adipose layer, derived by the division of SAT and identified as deep subcutaneous adipose tissue (dSAT), may play a significant and independent metabolic role. The aim of this study was to evaluate depot-specific differences in the expression of proteins key to adipocyte metabolism in a lean population to establish a potential physiologic role for dSAT. Research Methods and Procedures: Adipocytes and preadipocytes were isolated from whole biopsies taken from superficial SAT (sSAT), dSAT, and VAT samples obtained from 10 healthy normal weight patients (7 women and 3 men), with a mean age of 56.4 ± 4.04 years and a mean BMI of 23.1 ± 0.5 kg/m2. Samples were evaluated for depot-specific differences in insulin sensitivity using adiponectin, glucose transport protein 4 (GLUT4), and resistin mRNA and protein expression, glucocorticoid metabolism by 11β-hydroxysteroid dehydrogenase type-1 (11β-HSD1) expression, and alterations in the adipokines leptin and tumor necrosis factor-α (TNF-α). Results: Although no regional differences in expression were observed for adiponectin or TNF-α, dSAT whole biopsies and adipocytes, while intermediary to both sSAT and VAT, reflected more of the VAT expression profile of 11β-HSD1, leptin, and resistin. Only in the case of the intracellular pool of GLUT4 proteins in whole biopsies was an independent pattern of expression observed for dSAT. In an evaluation of the homeostatic model, dSAT 11β-HSD1 protein (r = 0.9573, p = 0.0002) and TNF-α mRNA (r = 0.8210, p = 0.0236) correlated positively to the homeostatic model. Discussion: Overall, dSAT seems to be a distinct abdominal adipose depot supporting an independent metabolic function that may have a potential role in the development of obesity-associated complications.

AB - Objective: Abdominal visceral (VAT) and subcutaneous adipose tissue (SAT) display significam metabolic differences, with VAT showing a functional association to metabolic/cardiovascular disorders. A third abdominal adipose layer, derived by the division of SAT and identified as deep subcutaneous adipose tissue (dSAT), may play a significant and independent metabolic role. The aim of this study was to evaluate depot-specific differences in the expression of proteins key to adipocyte metabolism in a lean population to establish a potential physiologic role for dSAT. Research Methods and Procedures: Adipocytes and preadipocytes were isolated from whole biopsies taken from superficial SAT (sSAT), dSAT, and VAT samples obtained from 10 healthy normal weight patients (7 women and 3 men), with a mean age of 56.4 ± 4.04 years and a mean BMI of 23.1 ± 0.5 kg/m2. Samples were evaluated for depot-specific differences in insulin sensitivity using adiponectin, glucose transport protein 4 (GLUT4), and resistin mRNA and protein expression, glucocorticoid metabolism by 11β-hydroxysteroid dehydrogenase type-1 (11β-HSD1) expression, and alterations in the adipokines leptin and tumor necrosis factor-α (TNF-α). Results: Although no regional differences in expression were observed for adiponectin or TNF-α, dSAT whole biopsies and adipocytes, while intermediary to both sSAT and VAT, reflected more of the VAT expression profile of 11β-HSD1, leptin, and resistin. Only in the case of the intracellular pool of GLUT4 proteins in whole biopsies was an independent pattern of expression observed for dSAT. In an evaluation of the homeostatic model, dSAT 11β-HSD1 protein (r = 0.9573, p = 0.0002) and TNF-α mRNA (r = 0.8210, p = 0.0236) correlated positively to the homeostatic model. Discussion: Overall, dSAT seems to be a distinct abdominal adipose depot supporting an independent metabolic function that may have a potential role in the development of obesity-associated complications.

KW - Adipocytes

KW - Deep subcutaneous

KW - Preadipocytes

KW - Superficial subcutaneous

KW - Visceral

UR - http://www.scopus.com/inward/record.url?scp=34548645016&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548645016&partnerID=8YFLogxK

U2 - 10.1038/oby.2007.231

DO - 10.1038/oby.2007.231

M3 - Article

C2 - 17712110

AN - SCOPUS:34548645016

VL - 15

SP - 1933

EP - 1943

JO - Obesity

JF - Obesity

SN - 1930-7381

IS - 8

ER -