Defective and nondefective adenovirus vectors for expressing foreign genes in vitro and in vivo

Massimo Levrero, Véronique Barban, Sylvie Manteca, Annick Ballay, Clara Balsamo, Maria Laura Avantaggiati, Gioacchino Natoli, Huub Skellekens, Pierre Tiollais, Michel Perricaudet

Research output: Contribution to journalArticlepeer-review

Abstract

We have constructed recombinant adenoviruses (Ad), with functional or defective Ela genes, which harbor either the hepatitis B (HB) virus s gene encoding the HB surface antigen, as well as the pre-S2 epitopes, or the bacterial gene encoding chloramphenicol acetyltransferase (CAT) under control of the Ad major late promoter (MLP). The recombinant viruses defective for Ela (Ad.MLP.S2 and Ad.CAT), which can be efficiently propagated only on 293 cells that complement this defect, and the nondefective (Ad.MLP.S2.E1A) recombinant were used to infect a wide spectrum of cells of different origin. The yields of HBs and CAT proteins obtained with these different recombinant viruses demonstrate no real advantage to using nondefective vectors, whatever the cell type infected. The injection into chimpanzees of Ad.MLP.S2 does not elicit the production of antibodies, but can immunologically prime the animals, resulting in a partial protection against HBV challenge.

Original languageEnglish
Pages (from-to)195-202
Number of pages8
JournalGene
Volume101
Issue number2
DOIs
Publication statusPublished - May 30 1991

Keywords

  • gene expression
  • major late promoter
  • Recombinant virus

ASJC Scopus subject areas

  • Genetics

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