Defective control of apoptosis, radiosensitivity, and spindle checkpoint in ataxia telangiectasia

Masatoshi Takagi, Domenico Delia, Luciana Chessa, Satoshi Iwata, Teruko Shigeta, Yusuke Kanke, Kumiko Goi, Minoru Asada, Mariko Eguchi, Chie Kodama, Shuki Mizutani

Research output: Contribution to journalArticle

Abstract

We examined the regulation of apoptosis, radiosensitivity, and spindle checkpoint in response to DNA-damaging agents in ataxia telangiectasia (AT)- derived lymphoblastoid cell lines (AT-LCLs), which lack AT mutated (ATM) protein expression. In addition to the previous findings that AT-LCLs are defective in regulation of cell cycle at the G1, S, and G2-M checkpoints in response to X-ray irradiation (X-IR) and are highly sensitive to X-IR (J. Biol. Chem., 271: 20486-20493, 1996), we showed for the first time that AT- LCLs were defective in X-IR-associated spindle checkpoint control. The cells were also resistant to early apoptosis as much as LCLs derived from patients with Li-Fraumeni syndrome (LFS-LCLs). Terminal deoxynucleotidyl transferase- mediated nick end labeling assay of LCLs, however, demonstrated a significant increase in apoptotic cells among AT-LCLs cultured over a longer period after X-IR. These findings were in contrast to those of LFS-LCL, which showed very little increase in terminal deoxynucleotidyl transferase-mediated nick end labeling-positive population, even in cells with hyperploidy. Thus, although early apoptosis and cell cycle controls in response to DNA damage are disrupted in both ATM and p53 mutations, cells from AT patients are much more susceptible to late-onset apoptosis than those of LFS. These differences may depend on the level of accumulation of DNA damage and/or threshold that triggers late-onset cell death in ATM or p53 mutations. Our findings allow a better understanding of the role of ATM in p53-dependent and independent signal transduction pathways in response to DNA damaging agents.

Original languageEnglish
Pages (from-to)4923-4929
Number of pages7
JournalCancer Research
Volume58
Issue number21
Publication statusPublished - Nov 1 1998

    Fingerprint

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Takagi, M., Delia, D., Chessa, L., Iwata, S., Shigeta, T., Kanke, Y., Goi, K., Asada, M., Eguchi, M., Kodama, C., & Mizutani, S. (1998). Defective control of apoptosis, radiosensitivity, and spindle checkpoint in ataxia telangiectasia. Cancer Research, 58(21), 4923-4929.