Defective expression of the apoptosis-inducing CD95 (Fas/APO-1) molecule on T and B cells in IDDM

C. Giordano, R. De Maria, G. Stassi, M. Todaro, P. Richiusa, M. Giordano, R. Testi, A. Galluzzo

Research output: Contribution to journalArticle

Abstract

Triggering of CD95 (Fas/APO-1) cell surface receptors regulates the elimination of autoreactive T and B lymphocytes through a mechanism of cell suicide called apoptosis. Three different mutations involving CD95 or its ligand are responsible for induction of autoimmunity in susceptible mouse strains. To determine whether a defect involving the CD95 receptor is associated with human insulin-dependent diabetes mellitus (IDDM), we have studied the expression of CD95 on peripheral blood mononuclear cells from IDDM patients at different stages of the disease. Three-colour flow cytometry and mean fluorescence analysis showed that T and B lymphocytes from newly diagnosed IDDM and patients with long-standing disease, and subjects at high risk of developing the disease were highly defective in CD95 expression (p+ CD4+ (p+ CD8+ cells (p range:

Original languageEnglish
Pages (from-to)1449-1454
Number of pages6
JournalDiabetologia
Volume38
Issue number12
DOIs
Publication statusPublished - Dec 1995

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Keywords

  • CD95
  • cellular immunity, apoptosis
  • Fas/APO1
  • insulin-dependent diabetes mellitus

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Giordano, C., De Maria, R., Stassi, G., Todaro, M., Richiusa, P., Giordano, M., Testi, R., & Galluzzo, A. (1995). Defective expression of the apoptosis-inducing CD95 (Fas/APO-1) molecule on T and B cells in IDDM. Diabetologia, 38(12), 1449-1454. https://doi.org/10.1007/BF00400606