Defective interleukin six expression and responsiveness in human mammary cells transformed by an adeno 5/SV40 hybrid virus

F. Basolo, L. Fiore, S. Calvo, V. Falcone, P. G. Conaldi, G. Fontanini, A. M. Caligo, G. Merlo, Y. Gluzman, A. Toniolo

Research output: Contribution to journalArticle

Abstract

Mammary epithelial cells (MECs) were isolated and cultured from mammary glands of healthy women undergoing reduction mammoplasty. Normal MECs were infected with the transforming hybrid virus adeno-5/SV40. Two transformed epithelial cell lines, M1 and M2, were obtained, characterised phenotypically and studied for the production of and the response to cytokines and growth regulators. In both cell lines, expression of the SV40 large T antigen was associated with loss of interleukin 6 (IL-6) production and responsiveness as well as with down-regulation of IL-8 and transforming growth factor (TGF)-α production. Both M1 and M2 cell lines were capable of forming colonies in semisolid media, but upon injection into severe combined immunodeficient (SCID) mice only M2 cells were tumorigenic. DNA synthesis in M1 cells was partially inhibited by serum or TNF-α and weakly stimulated by hydrocortisone (HC) and IL-8. In contrast, M2 cells were totally unresponsive to a variety of growth regulators. Both lines overexpressed the p53 protein at levels about 20-fold higher than those observed in primary MEC cultures, but no mutations of the p53 gene could be detected. The data confirm the view that the expression in human mammary cells of different oncogenes - including the SV40 T antigen - is frequently associated with alterations of cytokine production and responsiveness.

Original languageEnglish
Pages (from-to)1356-1361
Number of pages6
JournalBritish Journal of Cancer
Volume73
Issue number11
Publication statusPublished - 1996

Keywords

  • Cytokines
  • Interleukin 6
  • Mammary cells
  • SV40

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Basolo, F., Fiore, L., Calvo, S., Falcone, V., Conaldi, P. G., Fontanini, G., Caligo, A. M., Merlo, G., Gluzman, Y., & Toniolo, A. (1996). Defective interleukin six expression and responsiveness in human mammary cells transformed by an adeno 5/SV40 hybrid virus. British Journal of Cancer, 73(11), 1356-1361.