Deficit of IgG2 in HIV-positive pregnant women is responsible of inadequate IgG2 levels in their HIV-uninfected children in Malawi

Silvia Baroncelli, Clementina Maria Galluzzo, Giuseppe Liotta, Mauro Andreotti, Sandro Mancinelli, Robert Mphwere, Enok Bokola, Roberta Amici, Maria Cristina Marazzi, Leonardo Palombi, Francesca Lucaroni, Marina Giuliano

Research output: Contribution to journalArticle

Abstract

Background: Transplacental passage of IgGs is impaired in HIV + pregnant women, possibly determining an inadequate immunological protection in their children. We aimed to determine the impact of maternal immunological IgG profile and immunoactivation status on the efficiency of transplacental passage of IgG subclasses in HIV + mothers. Methods: 16 mother/infants pairs were studied in Malawi. Mothers received antiretroviral therapy (ART) from the third trimester of pregnancy. Determinations of pre-ART levels of maternal sCD14, of IgG subclasses in mothers at delivery and in their 1-month-old infants, were performed using commercial ELISA kits. Results: At delivery, after a median of 10 weeks of ART, 12/16 mothers were hypergammaglobulinemic, with IgG levels (20.5 mg/ml, 95% CI:18.8–26.8) directly correlated to the plasmatic levels of sCD14 (r = 0.640, p = 0.014). IgG1 levels (17.9 mg/ml) accounted for 82% of IgG, IgG3 and IgG4 levels were in the normal range. A profound deficit of IgG2 was observed both in mothers (0.60 mg/ml) and in infants (0.14 mg/ml). Placental transfer ratio (range 0.16–0.42) did not show a selective impairment between the different IgG subclasses. The transplacental passage of all IgG subclasses was decreased in the presence of maternal IgG over 16 mg/ml (significantly for IgG1, p = 0.031) and of high levels of sCD14 (p = 0.063). Conclusions: Transplacental passage was reduced for all IgG subclasses and inversely correlated to high levels of maternal IgGs and to the degree of immunoactivation. The profound depression of IgG2 in mothers suggests that IgG2 neonatal levels mostly reflect the maternal deficit rather than a selective impairment of IgG2 transfer.

Original languageEnglish
Pages (from-to)175-182
Number of pages8
JournalMedical Microbiology and Immunology
Volume207
Issue number3-4
DOIs
Publication statusPublished - Aug 1 2018

Fingerprint

Malawi
Pregnant Women
Immunoglobulin G
HIV
Mothers

Keywords

  • HIV
  • IgG subclasses
  • Malawi
  • Pregnant woman

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Microbiology (medical)

Cite this

Deficit of IgG2 in HIV-positive pregnant women is responsible of inadequate IgG2 levels in their HIV-uninfected children in Malawi. / Baroncelli, Silvia; Galluzzo, Clementina Maria; Liotta, Giuseppe; Andreotti, Mauro; Mancinelli, Sandro; Mphwere, Robert; Bokola, Enok; Amici, Roberta; Marazzi, Maria Cristina; Palombi, Leonardo; Lucaroni, Francesca; Giuliano, Marina.

In: Medical Microbiology and Immunology, Vol. 207, No. 3-4, 01.08.2018, p. 175-182.

Research output: Contribution to journalArticle

Baroncelli, Silvia ; Galluzzo, Clementina Maria ; Liotta, Giuseppe ; Andreotti, Mauro ; Mancinelli, Sandro ; Mphwere, Robert ; Bokola, Enok ; Amici, Roberta ; Marazzi, Maria Cristina ; Palombi, Leonardo ; Lucaroni, Francesca ; Giuliano, Marina. / Deficit of IgG2 in HIV-positive pregnant women is responsible of inadequate IgG2 levels in their HIV-uninfected children in Malawi. In: Medical Microbiology and Immunology. 2018 ; Vol. 207, No. 3-4. pp. 175-182.
@article{8ea178d01cb44203baf142d043db386e,
title = "Deficit of IgG2 in HIV-positive pregnant women is responsible of inadequate IgG2 levels in their HIV-uninfected children in Malawi",
abstract = "Background: Transplacental passage of IgGs is impaired in HIV + pregnant women, possibly determining an inadequate immunological protection in their children. We aimed to determine the impact of maternal immunological IgG profile and immunoactivation status on the efficiency of transplacental passage of IgG subclasses in HIV + mothers. Methods: 16 mother/infants pairs were studied in Malawi. Mothers received antiretroviral therapy (ART) from the third trimester of pregnancy. Determinations of pre-ART levels of maternal sCD14, of IgG subclasses in mothers at delivery and in their 1-month-old infants, were performed using commercial ELISA kits. Results: At delivery, after a median of 10 weeks of ART, 12/16 mothers were hypergammaglobulinemic, with IgG levels (20.5 mg/ml, 95{\%} CI:18.8–26.8) directly correlated to the plasmatic levels of sCD14 (r = 0.640, p = 0.014). IgG1 levels (17.9 mg/ml) accounted for 82{\%} of IgG, IgG3 and IgG4 levels were in the normal range. A profound deficit of IgG2 was observed both in mothers (0.60 mg/ml) and in infants (0.14 mg/ml). Placental transfer ratio (range 0.16–0.42) did not show a selective impairment between the different IgG subclasses. The transplacental passage of all IgG subclasses was decreased in the presence of maternal IgG over 16 mg/ml (significantly for IgG1, p = 0.031) and of high levels of sCD14 (p = 0.063). Conclusions: Transplacental passage was reduced for all IgG subclasses and inversely correlated to high levels of maternal IgGs and to the degree of immunoactivation. The profound depression of IgG2 in mothers suggests that IgG2 neonatal levels mostly reflect the maternal deficit rather than a selective impairment of IgG2 transfer.",
keywords = "HIV, IgG subclasses, Malawi, Pregnant woman",
author = "Silvia Baroncelli and Galluzzo, {Clementina Maria} and Giuseppe Liotta and Mauro Andreotti and Sandro Mancinelli and Robert Mphwere and Enok Bokola and Roberta Amici and Marazzi, {Maria Cristina} and Leonardo Palombi and Francesca Lucaroni and Marina Giuliano",
year = "2018",
month = "8",
day = "1",
doi = "10.1007/s00430-018-0537-2",
language = "English",
volume = "207",
pages = "175--182",
journal = "Medical Microbiology and Immunology",
issn = "0300-8584",
publisher = "Springer Verlag",
number = "3-4",

}

TY - JOUR

T1 - Deficit of IgG2 in HIV-positive pregnant women is responsible of inadequate IgG2 levels in their HIV-uninfected children in Malawi

AU - Baroncelli, Silvia

AU - Galluzzo, Clementina Maria

AU - Liotta, Giuseppe

AU - Andreotti, Mauro

AU - Mancinelli, Sandro

AU - Mphwere, Robert

AU - Bokola, Enok

AU - Amici, Roberta

AU - Marazzi, Maria Cristina

AU - Palombi, Leonardo

AU - Lucaroni, Francesca

AU - Giuliano, Marina

PY - 2018/8/1

Y1 - 2018/8/1

N2 - Background: Transplacental passage of IgGs is impaired in HIV + pregnant women, possibly determining an inadequate immunological protection in their children. We aimed to determine the impact of maternal immunological IgG profile and immunoactivation status on the efficiency of transplacental passage of IgG subclasses in HIV + mothers. Methods: 16 mother/infants pairs were studied in Malawi. Mothers received antiretroviral therapy (ART) from the third trimester of pregnancy. Determinations of pre-ART levels of maternal sCD14, of IgG subclasses in mothers at delivery and in their 1-month-old infants, were performed using commercial ELISA kits. Results: At delivery, after a median of 10 weeks of ART, 12/16 mothers were hypergammaglobulinemic, with IgG levels (20.5 mg/ml, 95% CI:18.8–26.8) directly correlated to the plasmatic levels of sCD14 (r = 0.640, p = 0.014). IgG1 levels (17.9 mg/ml) accounted for 82% of IgG, IgG3 and IgG4 levels were in the normal range. A profound deficit of IgG2 was observed both in mothers (0.60 mg/ml) and in infants (0.14 mg/ml). Placental transfer ratio (range 0.16–0.42) did not show a selective impairment between the different IgG subclasses. The transplacental passage of all IgG subclasses was decreased in the presence of maternal IgG over 16 mg/ml (significantly for IgG1, p = 0.031) and of high levels of sCD14 (p = 0.063). Conclusions: Transplacental passage was reduced for all IgG subclasses and inversely correlated to high levels of maternal IgGs and to the degree of immunoactivation. The profound depression of IgG2 in mothers suggests that IgG2 neonatal levels mostly reflect the maternal deficit rather than a selective impairment of IgG2 transfer.

AB - Background: Transplacental passage of IgGs is impaired in HIV + pregnant women, possibly determining an inadequate immunological protection in their children. We aimed to determine the impact of maternal immunological IgG profile and immunoactivation status on the efficiency of transplacental passage of IgG subclasses in HIV + mothers. Methods: 16 mother/infants pairs were studied in Malawi. Mothers received antiretroviral therapy (ART) from the third trimester of pregnancy. Determinations of pre-ART levels of maternal sCD14, of IgG subclasses in mothers at delivery and in their 1-month-old infants, were performed using commercial ELISA kits. Results: At delivery, after a median of 10 weeks of ART, 12/16 mothers were hypergammaglobulinemic, with IgG levels (20.5 mg/ml, 95% CI:18.8–26.8) directly correlated to the plasmatic levels of sCD14 (r = 0.640, p = 0.014). IgG1 levels (17.9 mg/ml) accounted for 82% of IgG, IgG3 and IgG4 levels were in the normal range. A profound deficit of IgG2 was observed both in mothers (0.60 mg/ml) and in infants (0.14 mg/ml). Placental transfer ratio (range 0.16–0.42) did not show a selective impairment between the different IgG subclasses. The transplacental passage of all IgG subclasses was decreased in the presence of maternal IgG over 16 mg/ml (significantly for IgG1, p = 0.031) and of high levels of sCD14 (p = 0.063). Conclusions: Transplacental passage was reduced for all IgG subclasses and inversely correlated to high levels of maternal IgGs and to the degree of immunoactivation. The profound depression of IgG2 in mothers suggests that IgG2 neonatal levels mostly reflect the maternal deficit rather than a selective impairment of IgG2 transfer.

KW - HIV

KW - IgG subclasses

KW - Malawi

KW - Pregnant woman

UR - http://www.scopus.com/inward/record.url?scp=85042612760&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85042612760&partnerID=8YFLogxK

U2 - 10.1007/s00430-018-0537-2

DO - 10.1007/s00430-018-0537-2

M3 - Article

C2 - 29488063

AN - SCOPUS:85042612760

VL - 207

SP - 175

EP - 182

JO - Medical Microbiology and Immunology

JF - Medical Microbiology and Immunology

SN - 0300-8584

IS - 3-4

ER -