Definition and characterization of an inhibitor for interleukin-31

Emilie Venereau, Caroline Diveu, Linda Grimaud, Elisa Ravon, Josy Froger, Laurence Preisser, Yannic Danger, Mike Maillasson, Laure Garrigue-Antar, Yannick Jacques, Sylvie Chevalier, Hugues Gascan

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Abstract

Interleukin-31 (IL-31) is a recently described T cell-derived cytokine, mainly produced by T helper type 2 cells and related to the IL-6 cytokine family according to its structure and receptor. IL-31 is the ligand for a heterodimeric receptor composed of a gp130-like receptor (GPL) associated with the oncostatin M receptor (OSMR). A link between IL-31 and atopic dermatitis was shown by studying the phenotype of IL-31 transgenic mice and IL-31 gene haplotypes in patients suffering from dermatitis. In this study, we generated a potent IL-31 antagonist formed by external portions of OSMR and GPL fused with a linker. This fusion protein, OSMR-LGPL, consisting of 720 amino acids, counteracted the binding of IL-31 to its membrane receptor complex and the subsequent signaling events involving the STATs and MAPK path-ways. Neutralizing effects were found in IL-31-sensitive cell lines, including brain-derived cells and primary cultures of keratinocytes.

Original languageEnglish
Pages (from-to)14955-14963
Number of pages9
JournalJournal of Biological Chemistry
Volume285
Issue number20
DOIs
Publication statusPublished - May 14 2010

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ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Venereau, E., Diveu, C., Grimaud, L., Ravon, E., Froger, J., Preisser, L., Danger, Y., Maillasson, M., Garrigue-Antar, L., Jacques, Y., Chevalier, S., & Gascan, H. (2010). Definition and characterization of an inhibitor for interleukin-31. Journal of Biological Chemistry, 285(20), 14955-14963. https://doi.org/10.1074/jbc.M109.049163