Definition of the transcription initiation site of human plasminogen gene in liver and non hepatic cell lines

Nicoletta Malgaretti, Ludovica Bruno, Marco Pontoglio, Giulia Candiani, Germana Meroni, Sergio Ottolenghi, Roberto Taramelli

Research output: Contribution to journalArticle

Abstract

We have mapped the cap site of the human plasminogen mRNA by primer extension and PCR techniques and found that it is located at position -161 relative to the first ATG, 97 bases upstream to the 5′ end of the previously isolated cDNA clone. Seven human hepatic and non hepatic cell lines and fresh liver cells were tested for human plasminogen mRNA expression: the liver and the liver derived HepG2 cell line represent the major site of plaminogen RNA synthesis while the other cell lines (Hep3B, HeLa, IMR, 293 Caco and SW626) show much lower levels.

Original languageEnglish
Pages (from-to)1013-1018
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume173
Issue number3
DOIs
Publication statusPublished - Dec 31 1990

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint Dive into the research topics of 'Definition of the transcription initiation site of human plasminogen gene in liver and non hepatic cell lines'. Together they form a unique fingerprint.

  • Cite this