TY - JOUR
T1 - Definition of the transcription initiation site of human plasminogen gene in liver and non hepatic cell lines
AU - Malgaretti, Nicoletta
AU - Bruno, Ludovica
AU - Pontoglio, Marco
AU - Candiani, Giulia
AU - Meroni, Germana
AU - Ottolenghi, Sergio
AU - Taramelli, Roberto
PY - 1990/12/31
Y1 - 1990/12/31
N2 - We have mapped the cap site of the human plasminogen mRNA by primer extension and PCR techniques and found that it is located at position -161 relative to the first ATG, 97 bases upstream to the 5′ end of the previously isolated cDNA clone. Seven human hepatic and non hepatic cell lines and fresh liver cells were tested for human plasminogen mRNA expression: the liver and the liver derived HepG2 cell line represent the major site of plaminogen RNA synthesis while the other cell lines (Hep3B, HeLa, IMR, 293 Caco and SW626) show much lower levels.
AB - We have mapped the cap site of the human plasminogen mRNA by primer extension and PCR techniques and found that it is located at position -161 relative to the first ATG, 97 bases upstream to the 5′ end of the previously isolated cDNA clone. Seven human hepatic and non hepatic cell lines and fresh liver cells were tested for human plasminogen mRNA expression: the liver and the liver derived HepG2 cell line represent the major site of plaminogen RNA synthesis while the other cell lines (Hep3B, HeLa, IMR, 293 Caco and SW626) show much lower levels.
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U2 - 10.1016/S0006-291X(05)80887-3
DO - 10.1016/S0006-291X(05)80887-3
M3 - Article
C2 - 2268308
AN - SCOPUS:0025552040
VL - 173
SP - 1013
EP - 1018
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -