Abstract
To investigate on the hypothetical presence of an antiapoptotic gene, we utilized the CODEHOP (COnsensus-DEgenerate Hybrid Oligonucleotide Primers) strategy amplifying unknown sequences from a background of genomic (bovine herpesvirus type-1) BHV-1 DNA. An alignment of carboxyl-terminal domains belonging to three proteins encoded by γ34.5, MyD116 and GADD34 genes, was carried out to design degenerate PCR primers in highly conserved regions. This allowed the amplification of a 110 bp fragment. This fragment was subjected to automatic sequencing and DNAsequence analysis revealed that its position resided between the nt 14363 and the nt 14438 in bovine herpesvirus type-1 (BHV-1) Cooper strain sharing an identity of 86% (UL14). Transient transfections showed that UL14 protein is efficient in protecting MDBK and K562 cells from sorbitol induced apoptosis. The protein's anti-apoptotic function may derive from its heat shock protein-like properties.
Original language | English |
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Pages (from-to) | 813-823 |
Number of pages | 11 |
Journal | Journal of Cellular Biochemistry |
Volume | 97 |
Issue number | 4 |
DOIs | |
Publication status | Published - Mar 1 2006 |
Keywords
- Apoptosis
- BHV-1
- CODEHOP
- PCR degenerate
- UL14
ASJC Scopus subject areas
- Biochemistry
- Cell Biology