Delayed Luminescence technique: A probe for vital processes in biological systems

M. Ballerini, M. Milani, L. Ferraro, M. Zabeo, F. Musumeci

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Delayed Luminescence is a well established technique based on the illumination of a biological sample and on the subsequent count of the number of photons re-emitted by the sample after the light source has been switched off together with their spectral distribution. Investigations have been performed on yeast cells and algae so that correlations can be established between biological activities and physical parameters of the samples. Moreover nonlinear mechanisms of interaction between optical fields and cells can be figured out. Further investigations will be reported on yeast cell samples deposited on paper filters after irradiation by soft X-rays. The results will be discussed by cross correlating the experimental evidence from Delayed Luminescence with those obtained by metabolic activity recording. Luminescence and Delayed Luminescence are strictly correlated with early insurgence of morphological alterations of normal or pathological nature in cells and tissues; a novel technique for morphological analysis has been developed by means of Focused Ion Beam machines. A straightforward approach to morphology at the nanoscale both of membranes and cellular inner structures is then made possible. The final aim is an experimental set up for an early and reliable detection technique for neoplastic cells and tissues sorting.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsD.L. Farkas, R.C. Leif
Pages262-271
Number of pages10
Volume4622
DOIs
Publication statusPublished - 2002
EventOptical Diagnostics of Living Cells V - San Jose, CA, United States
Duration: Jan 23 2002Jan 25 2002

Other

OtherOptical Diagnostics of Living Cells V
Country/TerritoryUnited States
CitySan Jose, CA
Period1/23/021/25/02

Keywords

  • Bioluminescence
  • Cell metabolism
  • Cell morphology
  • Delayed Luminescence
  • Focused Ion Beam imaging
  • Tumour cells

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Fingerprint

Dive into the research topics of 'Delayed Luminescence technique: A probe for vital processes in biological systems'. Together they form a unique fingerprint.

Cite this