Delayed Luminescence technique

A probe for vital processes in biological systems

M. Ballerini, M. Milani, L. Ferraro, M. Zabeo, F. Musumeci

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Delayed Luminescence is a well established technique based on the illumination of a biological sample and on the subsequent count of the number of photons re-emitted by the sample after the light source has been switched off together with their spectral distribution. Investigations have been performed on yeast cells and algae so that correlations can be established between biological activities and physical parameters of the samples. Moreover nonlinear mechanisms of interaction between optical fields and cells can be figured out. Further investigations will be reported on yeast cell samples deposited on paper filters after irradiation by soft X-rays. The results will be discussed by cross correlating the experimental evidence from Delayed Luminescence with those obtained by metabolic activity recording. Luminescence and Delayed Luminescence are strictly correlated with early insurgence of morphological alterations of normal or pathological nature in cells and tissues; a novel technique for morphological analysis has been developed by means of Focused Ion Beam machines. A straightforward approach to morphology at the nanoscale both of membranes and cellular inner structures is then made possible. The final aim is an experimental set up for an early and reliable detection technique for neoplastic cells and tissues sorting.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsD.L. Farkas, R.C. Leif
Pages262-271
Number of pages10
Volume4622
DOIs
Publication statusPublished - 2002
EventOptical Diagnostics of Living Cells V - San Jose, CA, United States
Duration: Jan 23 2002Jan 25 2002

Other

OtherOptical Diagnostics of Living Cells V
CountryUnited States
CitySan Jose, CA
Period1/23/021/25/02

Fingerprint

Biological systems
Luminescence
luminescence
probes
yeast
Yeast
cells
Cells
Tissue
Focused ion beams
algae
Algae
activity (biology)
Bioactivity
classifying
Sorting
Light sources
light sources
Photons
Lighting

Keywords

  • Bioluminescence
  • Cell metabolism
  • Cell morphology
  • Delayed Luminescence
  • Focused Ion Beam imaging
  • Tumour cells

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Ballerini, M., Milani, M., Ferraro, L., Zabeo, M., & Musumeci, F. (2002). Delayed Luminescence technique: A probe for vital processes in biological systems. In D. L. Farkas, & R. C. Leif (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4622, pp. 262-271) https://doi.org/10.1117/12.468351

Delayed Luminescence technique : A probe for vital processes in biological systems. / Ballerini, M.; Milani, M.; Ferraro, L.; Zabeo, M.; Musumeci, F.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / D.L. Farkas; R.C. Leif. Vol. 4622 2002. p. 262-271.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ballerini, M, Milani, M, Ferraro, L, Zabeo, M & Musumeci, F 2002, Delayed Luminescence technique: A probe for vital processes in biological systems. in DL Farkas & RC Leif (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4622, pp. 262-271, Optical Diagnostics of Living Cells V, San Jose, CA, United States, 1/23/02. https://doi.org/10.1117/12.468351
Ballerini M, Milani M, Ferraro L, Zabeo M, Musumeci F. Delayed Luminescence technique: A probe for vital processes in biological systems. In Farkas DL, Leif RC, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 4622. 2002. p. 262-271 https://doi.org/10.1117/12.468351
Ballerini, M. ; Milani, M. ; Ferraro, L. ; Zabeo, M. ; Musumeci, F. / Delayed Luminescence technique : A probe for vital processes in biological systems. Proceedings of SPIE - The International Society for Optical Engineering. editor / D.L. Farkas ; R.C. Leif. Vol. 4622 2002. pp. 262-271
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