Deletion mapping on the long arm of chromosome 7 in splenic lymphoma with villous lymphocytes

Alicja M. Gruszka-Westwood, Rifat Hamoudi, Lucy Osborne, Estella Matutes, Daniel Catovsky

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Splenic lymphoma with villous lymphocytes (SLVL) is a low-grade lymphoproliferative disorder characterized by splenomegaly and circulating villous lymphocytes in the peripheral blood. It is considered to be the leukemic form of splenic marginal zone lymphoma (SMZL). The genetic basis of this lymphoma type remains unknown. Conventional cytogenetic studies have identified frequent structural abnormalities of chromosome 7, in the form of translocations, mainly unbalanced, and 7q deletions. In this current study, we undertook deletion mapping of the long arm of chromosome 7 in a series of cases with SLVL. Metaphase fluorescence in situ hybridization (FISH) was used in the first instance, followed by a study of loss of heterozygosity (LOH). The common area of deletion identified by FISH spanned from the YAC clone HSC7E1289 (mapping to 7q32.1) to in between YACs HSC7E195 and HSC7E648 (7q32-3). By application of 50 microsatellite markers mapping to the FISH-CDR and to areas of deletion reported in other studies, four distinct hotspot loci were identified, with abnormalities present in 29-55% cases. In three of them, both LOH and biallelic deletions were found. The LOH in the majority of patients was noncontiguous. The presence of a high incidence of abnormalities in the established hotspot areas and in particular the finding of biallelic deletions is indicative of the existence of genes important for the pathogenesis of SLVL in these areas.

Original languageEnglish
Pages (from-to)57-69
Number of pages13
JournalGenes Chromosomes and Cancer
Volume36
Issue number1
DOIs
Publication statusPublished - Jan 1 2003

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Chromosomes, Human, Pair 7
Lymphoma
Lymphocytes
Loss of Heterozygosity
Fluorescence In Situ Hybridization
Lymphoproliferative Disorders
Splenomegaly
Metaphase
Cytogenetics
Microsatellite Repeats
Clone Cells
Incidence
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Genetics

Cite this

Deletion mapping on the long arm of chromosome 7 in splenic lymphoma with villous lymphocytes. / Gruszka-Westwood, Alicja M.; Hamoudi, Rifat; Osborne, Lucy; Matutes, Estella; Catovsky, Daniel.

In: Genes Chromosomes and Cancer, Vol. 36, No. 1, 01.01.2003, p. 57-69.

Research output: Contribution to journalArticle

Gruszka-Westwood, Alicja M. ; Hamoudi, Rifat ; Osborne, Lucy ; Matutes, Estella ; Catovsky, Daniel. / Deletion mapping on the long arm of chromosome 7 in splenic lymphoma with villous lymphocytes. In: Genes Chromosomes and Cancer. 2003 ; Vol. 36, No. 1. pp. 57-69.
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