Delineation and physical separation of novel translocation breakpoints on chromosome 1p in two genetically closely associated childhood tumors

M. J C Steenman, N. Zijlstra, D. L. Kruitbosch, C. Wiesmeijer, L. Larizza, P. A. Voûte, A. Westerveld, M. M A M Mannens

Research output: Contribution to journalArticle

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Abstract

Sporadic childhood tumors associated with Beckwith-Wiedemann syndrome (BWS) all show abnormalities of the same region on chromosome 11. In addition to chromosome 11, other chromosome regions are affected in some of these tumor types. In this study we analyzed the region on chromosome 1p involved in the etiology of BWS-associated tumors, Wilms tumor, rhabdomyosarcoma, and hepatoblastoma. For this purpose we determined the location of two novel translocation breakpoints in this chromosome region in cells from a Wilms tumor and cells from a rhabdomyosarcoma. We constructed a map of the region and found that both breakpoints are separated by at least 875 kb. We identified a PAC clone which crosses the rhabdomyosarcoma breakpoint and found several exons within this clone. We established that this breakpoint is located proximal to the PAX7 gene and, therefore, identified a new region involved in the etiology of rhabdomyosarcomas. Copyright (C) 2000 S. Karger AG, Basel.

Original languageEnglish
Pages (from-to)289-295
Number of pages7
JournalCytogenetics and Cell Genetics
Volume88
Issue number3-4
Publication statusPublished - 2000

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Chromosome Breakpoints
Rhabdomyosarcoma
Beckwith-Wiedemann Syndrome
Chromosomes, Human, Pair 11
Wilms Tumor
Neoplasms
Clone Cells
Chromosomes
Hepatoblastoma
Exons
Genes

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Steenman, M. J. C., Zijlstra, N., Kruitbosch, D. L., Wiesmeijer, C., Larizza, L., Voûte, P. A., ... Mannens, M. M. A. M. (2000). Delineation and physical separation of novel translocation breakpoints on chromosome 1p in two genetically closely associated childhood tumors. Cytogenetics and Cell Genetics, 88(3-4), 289-295.

Delineation and physical separation of novel translocation breakpoints on chromosome 1p in two genetically closely associated childhood tumors. / Steenman, M. J C; Zijlstra, N.; Kruitbosch, D. L.; Wiesmeijer, C.; Larizza, L.; Voûte, P. A.; Westerveld, A.; Mannens, M. M A M.

In: Cytogenetics and Cell Genetics, Vol. 88, No. 3-4, 2000, p. 289-295.

Research output: Contribution to journalArticle

Steenman, MJC, Zijlstra, N, Kruitbosch, DL, Wiesmeijer, C, Larizza, L, Voûte, PA, Westerveld, A & Mannens, MMAM 2000, 'Delineation and physical separation of novel translocation breakpoints on chromosome 1p in two genetically closely associated childhood tumors', Cytogenetics and Cell Genetics, vol. 88, no. 3-4, pp. 289-295.
Steenman, M. J C ; Zijlstra, N. ; Kruitbosch, D. L. ; Wiesmeijer, C. ; Larizza, L. ; Voûte, P. A. ; Westerveld, A. ; Mannens, M. M A M. / Delineation and physical separation of novel translocation breakpoints on chromosome 1p in two genetically closely associated childhood tumors. In: Cytogenetics and Cell Genetics. 2000 ; Vol. 88, No. 3-4. pp. 289-295.
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AB - Sporadic childhood tumors associated with Beckwith-Wiedemann syndrome (BWS) all show abnormalities of the same region on chromosome 11. In addition to chromosome 11, other chromosome regions are affected in some of these tumor types. In this study we analyzed the region on chromosome 1p involved in the etiology of BWS-associated tumors, Wilms tumor, rhabdomyosarcoma, and hepatoblastoma. For this purpose we determined the location of two novel translocation breakpoints in this chromosome region in cells from a Wilms tumor and cells from a rhabdomyosarcoma. We constructed a map of the region and found that both breakpoints are separated by at least 875 kb. We identified a PAC clone which crosses the rhabdomyosarcoma breakpoint and found several exons within this clone. We established that this breakpoint is located proximal to the PAX7 gene and, therefore, identified a new region involved in the etiology of rhabdomyosarcomas. Copyright (C) 2000 S. Karger AG, Basel.

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