Demonstration of epidermal growth factor receptor in colorectal adenocarcinoma by enzyme immunoassay

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Abstract

The epidermal growth factor receptor (EGFR) was investigated by an enzyme immunoassay in neoplastic tissue and surrounding mucosa from 43 patients with colorectal cancer. This study found a positivity of 86% in normal samples and a positivity of 93% in neoplastic ones. In normal tissue samples, EGFR levels were 162 ± 129 ng Ab B/mg protein, while in colorectal neoplastic samples EGFR levels were 228 ± 135 ng Ab B/mg protein. This difference was statistically significant (p <0.01). There was a significant correlation between the EGFR levels in neoplastic tissue and those in surrounding mucosa (r = 0.75). In 5 of 43 patients, the EGFR was evaluated also by Western blotting with three different anti-EGFR antibodies, of which one was biotin-conjugated. The presence of a 170-kDa band was detected. Moreover, by using the anti-EGFR mAb biotin conjugate a 90-kDa band was also detected.

Original languageEnglish
Pages (from-to)103-107
Number of pages5
JournalDigestion
Volume55
Issue number2
DOIs
Publication statusPublished - 1994

Keywords

  • Colorectal adenocarcinoma
  • Enzyme immunoassay
  • Epidermal growth factor receptor

ASJC Scopus subject areas

  • Gastroenterology

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