Demonstration of low density lipoprotein receptor in human colonic carcinoma and surrounding mucosa by immunoenzymatic assay

M. G. Caruso, M. Notarnicola, A. Cavallini, V. Guerra, G. Misciagna, A. Di Leo

Research output: Contribution to journalArticle

Abstract

In order to verify the presence of Low Density Lipoprotein Receptor (LDLR) in cellular membranes isolated from human colonic tissue, samples from the neoplastic colorectum and the normal surrounding mucosa were studied by an enzyme-linked-immunosorbant assay. A monoclonal antibody against the human LDLR was used. The LDLR content revealed a considerable inter-individual variation. In 36 out of 53 cases (68%) there was no LDLR presence in either normal or neoplastic tissue samples. In 5 out of 53 cases (9.4%), LDLRs were detected in both types of tissue samples, in 9 cases out of 53 (17%) LDLRs were present in neoplastic tissue, while in 3 out of 53 cases (5.6%) only in normal mucosa. The anti-LDLR mono-clonal antibody (mAb) binding was significantly higher in neoplastic tissue samples than normal surrounding colonic mucosa ones. Sex, age, body mass index (BMI), serum cholesterol, tumour site, histologic grading and Dukes' stage did not seem to be associated with LDLR presence.

Original languageEnglish
Pages (from-to)361-367
Number of pages7
JournalItalian Journal of Gastroenterology
Volume25
Issue number7
Publication statusPublished - 1993

Fingerprint

LDL Receptors
Mucous Membrane
Carcinoma
Body Mass Index
Cholesterol
Monoclonal Antibodies
human LDLR protein
Membranes
Antibodies
Enzymes
Serum
Neoplasms

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Demonstration of low density lipoprotein receptor in human colonic carcinoma and surrounding mucosa by immunoenzymatic assay. / Caruso, M. G.; Notarnicola, M.; Cavallini, A.; Guerra, V.; Misciagna, G.; Di Leo, A.

In: Italian Journal of Gastroenterology, Vol. 25, No. 7, 1993, p. 361-367.

Research output: Contribution to journalArticle

@article{abe52bf9932543e5b2bf72e0fdea7c8b,
title = "Demonstration of low density lipoprotein receptor in human colonic carcinoma and surrounding mucosa by immunoenzymatic assay",
abstract = "In order to verify the presence of Low Density Lipoprotein Receptor (LDLR) in cellular membranes isolated from human colonic tissue, samples from the neoplastic colorectum and the normal surrounding mucosa were studied by an enzyme-linked-immunosorbant assay. A monoclonal antibody against the human LDLR was used. The LDLR content revealed a considerable inter-individual variation. In 36 out of 53 cases (68{\%}) there was no LDLR presence in either normal or neoplastic tissue samples. In 5 out of 53 cases (9.4{\%}), LDLRs were detected in both types of tissue samples, in 9 cases out of 53 (17{\%}) LDLRs were present in neoplastic tissue, while in 3 out of 53 cases (5.6{\%}) only in normal mucosa. The anti-LDLR mono-clonal antibody (mAb) binding was significantly higher in neoplastic tissue samples than normal surrounding colonic mucosa ones. Sex, age, body mass index (BMI), serum cholesterol, tumour site, histologic grading and Dukes' stage did not seem to be associated with LDLR presence.",
author = "Caruso, {M. G.} and M. Notarnicola and A. Cavallini and V. Guerra and G. Misciagna and {Di Leo}, A.",
year = "1993",
language = "English",
volume = "25",
pages = "361--367",
journal = "Italian Journal of Gastroenterology",
issn = "0392-0623",
publisher = "Piccin Editore",
number = "7",

}

TY - JOUR

T1 - Demonstration of low density lipoprotein receptor in human colonic carcinoma and surrounding mucosa by immunoenzymatic assay

AU - Caruso, M. G.

AU - Notarnicola, M.

AU - Cavallini, A.

AU - Guerra, V.

AU - Misciagna, G.

AU - Di Leo, A.

PY - 1993

Y1 - 1993

N2 - In order to verify the presence of Low Density Lipoprotein Receptor (LDLR) in cellular membranes isolated from human colonic tissue, samples from the neoplastic colorectum and the normal surrounding mucosa were studied by an enzyme-linked-immunosorbant assay. A monoclonal antibody against the human LDLR was used. The LDLR content revealed a considerable inter-individual variation. In 36 out of 53 cases (68%) there was no LDLR presence in either normal or neoplastic tissue samples. In 5 out of 53 cases (9.4%), LDLRs were detected in both types of tissue samples, in 9 cases out of 53 (17%) LDLRs were present in neoplastic tissue, while in 3 out of 53 cases (5.6%) only in normal mucosa. The anti-LDLR mono-clonal antibody (mAb) binding was significantly higher in neoplastic tissue samples than normal surrounding colonic mucosa ones. Sex, age, body mass index (BMI), serum cholesterol, tumour site, histologic grading and Dukes' stage did not seem to be associated with LDLR presence.

AB - In order to verify the presence of Low Density Lipoprotein Receptor (LDLR) in cellular membranes isolated from human colonic tissue, samples from the neoplastic colorectum and the normal surrounding mucosa were studied by an enzyme-linked-immunosorbant assay. A monoclonal antibody against the human LDLR was used. The LDLR content revealed a considerable inter-individual variation. In 36 out of 53 cases (68%) there was no LDLR presence in either normal or neoplastic tissue samples. In 5 out of 53 cases (9.4%), LDLRs were detected in both types of tissue samples, in 9 cases out of 53 (17%) LDLRs were present in neoplastic tissue, while in 3 out of 53 cases (5.6%) only in normal mucosa. The anti-LDLR mono-clonal antibody (mAb) binding was significantly higher in neoplastic tissue samples than normal surrounding colonic mucosa ones. Sex, age, body mass index (BMI), serum cholesterol, tumour site, histologic grading and Dukes' stage did not seem to be associated with LDLR presence.

UR - http://www.scopus.com/inward/record.url?scp=0027444910&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027444910&partnerID=8YFLogxK

M3 - Article

C2 - 8280896

AN - SCOPUS:0027444910

VL - 25

SP - 361

EP - 367

JO - Italian Journal of Gastroenterology

JF - Italian Journal of Gastroenterology

SN - 0392-0623

IS - 7

ER -