Dendritic cells as natural adjuvants

Stefania Citterio, Maria Rescigno, Maria Foti, Francesca Granucci, Diego Aggujaro, Cristina Gasperi, Malgorzata K. Matyszak, Giampiero Girolomoni, Paola Ricciardi-Castagnoli

Research output: Contribution to journalArticlepeer-review


Dendritic cells (DCs) are professional antigen presenting cells that hold the key to the induction of T-cell responses. Therefore, the use of DCs for immunotherapy to stimulate immune responses has recently raised a great deal of interest. Many clinical trials using DCs have been initiated to stimulate immune responses against tumors or infectious agents. Several issues need to be considered before DCs can be used successfully as natural adjuvants: DCs have to be generated in sufficient numbers; they should display morphological, phenotypical, and functional properties of DCs; and they should be able to present antigens. In the present review we focus on methods for the purification of DCs from human bone marrow and peripheral blood and for the optimization of in vitro cell culture systems. Methods to generate growth factor-dependent mouse DC lines are also described.

Original languageEnglish
Pages (from-to)142-147
Number of pages6
JournalMethods in Enzymology
Issue number1
Publication statusPublished - Sep 1999

ASJC Scopus subject areas

  • Molecular Biology


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