Abstract
Human monocytes can differentiate into dendritic cells (DCs) according to the nature of environmental signals. We tested here whether the infection with the live tuberculosis vaccine bacillus Calmette-Guerin (BCG), which is known to be limited in preventing pulmonary tuberculosis, modulates monocyte and DC differentiation. We found that monocytes infected with BCG differentiate into CD1a- DCs (BCG-DCs) in the presence of granulocyte macrophage-colony stimulating factor and interleukin (IL)-4 and acquired a mature phenotype in the absence of maturation stimuli. In addition, BCG-DCs produced proinflammatory cytokines (tumor necrosis factor α, IL-1β, IL-6) and IL-10 but not IL-12. BCG-DCs were able to stimulate allogeneic T lymphocytes to a similar degree as DCs generated in the absence of infection. However, BCG-DCs induced IL-4 production when cocultured with human cord-blood mononuclear cells. The induction of IL-4 production by DCs generated by BCG-infected monocytes could explain the failure of the BCG vaccine to prevent pulmonary tuberculosis.
| Original language | English |
|---|---|
| Pages (from-to) | 827-834 |
| Number of pages | 8 |
| Journal | Journal of Leukocyte Biology |
| Volume | 76 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Oct 2004 |
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Keywords
- CD1a
- Cytokines
- Differentiation
- Mycobacteria
- Polarization
ASJC Scopus subject areas
- Cell Biology
Cite this
Dendritic cells derived from BCG-infected precursors induce Th2-like immune response. / Martino, Angelo; Sacchi, Alessandra; Sanarico, Nunzia; Spadaro, Francesca; Ramoni, Carlo; Ciaramella, Antonio; Pucillo, Leopoldo Paolo; Colizzi, Vittorio; Vendetti, Silvia.
In: Journal of Leukocyte Biology, Vol. 76, No. 4, 10.2004, p. 827-834.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dendritic cells derived from BCG-infected precursors induce Th2-like immune response
AU - Martino, Angelo
AU - Sacchi, Alessandra
AU - Sanarico, Nunzia
AU - Spadaro, Francesca
AU - Ramoni, Carlo
AU - Ciaramella, Antonio
AU - Pucillo, Leopoldo Paolo
AU - Colizzi, Vittorio
AU - Vendetti, Silvia
PY - 2004/10
Y1 - 2004/10
N2 - Human monocytes can differentiate into dendritic cells (DCs) according to the nature of environmental signals. We tested here whether the infection with the live tuberculosis vaccine bacillus Calmette-Guerin (BCG), which is known to be limited in preventing pulmonary tuberculosis, modulates monocyte and DC differentiation. We found that monocytes infected with BCG differentiate into CD1a- DCs (BCG-DCs) in the presence of granulocyte macrophage-colony stimulating factor and interleukin (IL)-4 and acquired a mature phenotype in the absence of maturation stimuli. In addition, BCG-DCs produced proinflammatory cytokines (tumor necrosis factor α, IL-1β, IL-6) and IL-10 but not IL-12. BCG-DCs were able to stimulate allogeneic T lymphocytes to a similar degree as DCs generated in the absence of infection. However, BCG-DCs induced IL-4 production when cocultured with human cord-blood mononuclear cells. The induction of IL-4 production by DCs generated by BCG-infected monocytes could explain the failure of the BCG vaccine to prevent pulmonary tuberculosis.
AB - Human monocytes can differentiate into dendritic cells (DCs) according to the nature of environmental signals. We tested here whether the infection with the live tuberculosis vaccine bacillus Calmette-Guerin (BCG), which is known to be limited in preventing pulmonary tuberculosis, modulates monocyte and DC differentiation. We found that monocytes infected with BCG differentiate into CD1a- DCs (BCG-DCs) in the presence of granulocyte macrophage-colony stimulating factor and interleukin (IL)-4 and acquired a mature phenotype in the absence of maturation stimuli. In addition, BCG-DCs produced proinflammatory cytokines (tumor necrosis factor α, IL-1β, IL-6) and IL-10 but not IL-12. BCG-DCs were able to stimulate allogeneic T lymphocytes to a similar degree as DCs generated in the absence of infection. However, BCG-DCs induced IL-4 production when cocultured with human cord-blood mononuclear cells. The induction of IL-4 production by DCs generated by BCG-infected monocytes could explain the failure of the BCG vaccine to prevent pulmonary tuberculosis.
KW - CD1a
KW - Cytokines
KW - Differentiation
KW - Mycobacteria
KW - Polarization
UR - http://www.scopus.com/inward/record.url?scp=4744366784&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=4744366784&partnerID=8YFLogxK
U2 - 10.1189/jlb.0703313
DO - 10.1189/jlb.0703313
M3 - Article
C2 - 15240755
AN - SCOPUS:4744366784
VL - 76
SP - 827
EP - 834
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
SN - 0741-5400
IS - 4
ER -