Dendritic cells from mice lacking the invariant chain express high levels of membrane MHC class II molecules in vivo

Patrizia Rovere, Frédérique Forquet, Valérie S. Zimmermann, Jeannine Trucy, Paola Ricciardi-Castagnoli, Jean Davoust

Research output: Contribution to journalArticle

Abstract

We investigated in H-2(k) mice bearing a genetically disrupted invariant chain (Ii) gene, the MHC class II expression and antigen presentation ability of dendritic cells (DC) freshly purified from the spleen (SpDC) or derived from bone marrow precursors (BMDC) upon treatment with GM-CSF. In the absence of Ii, class II α/β heterodimers are expressed on the DC membranes to a similar extent that in control mice, in contrast to splenic B cells. Class II molecules immunoprecipitated from the plasma membrane of Ii deficient DC are compact indicating that the dimers are stabilized by antigenic peptides. Furthermore DC from Ii mutant mice are able to present to CD4+ T lymphocytes, epitopes derived from the processing of the hen egg lysozyme (HEL) that normally require expression of the Ii molecule for presentation by B cells. All together, our results show that the antigen processing machinery of DC provides peptides that can reach class II molecules and stabilize their conformation in the absence of Ii mediated targeting of class II complexes.

Original languageEnglish
Pages (from-to)195-201
Number of pages7
JournalAdvances in Experimental Medicine and Biology
Volume417
Publication statusPublished - 1997

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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