Dendritic cells loaded with apoptotic oligodendrocytes as a source of myelin T-cell epitopes in multiple sclerosis

Francesca Meloni, Daniele Accapezzato, Cristina Agresti, Francesca Aloisi, Giovanni Ristori, Marco Salvetti, Roberto Furlan, Gianvito Martino, Vincenzo Barnaba, Marino Paroli

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Evidence suggests that T-cell response to myelin basic protein (MBP) plays an important role in multiple sclerosis (MS). However, the mechanism of generation for MBP immunogenic epitopes is unclear. A series of specific CD4+ T-cell lines was obtained by stimulating peripheral blood mononuclear cells from MS patients with synthetic peptides spanning the entire MBP sequence. T-cell lines recognizing MBP(8-27), MBP(13-32), and MBP(23-42) peptides, whose sequences are identical for humans and rats, specifically proliferated and produced large amounts of interferon-γ in response to autologous dendritic cells (DCs) loaded in vitro with apoptotic rat oligodendrocytes. Results suggest that MBP epitopes generated from enzymatic processing of apoptotic glial cells by DCs might be relevant to MS pathogenesis.

Original languageEnglish
Pages (from-to)286-294
Number of pages9
JournalClinical Immunology
Volume129
Issue number2
DOIs
Publication statusPublished - Nov 2008

Fingerprint

T-Lymphocyte Epitopes
Myelin Basic Protein
Oligodendroglia
Myelin Sheath
Dendritic Cells
Multiple Sclerosis
T-Lymphocytes
Epitopes
Cell Line
Peptides
Neuroglia
Interferons
Blood Cells

Keywords

  • Apoptosis
  • Dendritic cells
  • Multiple sclerosis
  • Myelin basic protein
  • Oligodendrocytes
  • T lymphocytes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Meloni, F., Accapezzato, D., Agresti, C., Aloisi, F., Ristori, G., Salvetti, M., ... Paroli, M. (2008). Dendritic cells loaded with apoptotic oligodendrocytes as a source of myelin T-cell epitopes in multiple sclerosis. Clinical Immunology, 129(2), 286-294. https://doi.org/10.1016/j.clim.2008.07.017

Dendritic cells loaded with apoptotic oligodendrocytes as a source of myelin T-cell epitopes in multiple sclerosis. / Meloni, Francesca; Accapezzato, Daniele; Agresti, Cristina; Aloisi, Francesca; Ristori, Giovanni; Salvetti, Marco; Furlan, Roberto; Martino, Gianvito; Barnaba, Vincenzo; Paroli, Marino.

In: Clinical Immunology, Vol. 129, No. 2, 11.2008, p. 286-294.

Research output: Contribution to journalArticle

Meloni, F, Accapezzato, D, Agresti, C, Aloisi, F, Ristori, G, Salvetti, M, Furlan, R, Martino, G, Barnaba, V & Paroli, M 2008, 'Dendritic cells loaded with apoptotic oligodendrocytes as a source of myelin T-cell epitopes in multiple sclerosis', Clinical Immunology, vol. 129, no. 2, pp. 286-294. https://doi.org/10.1016/j.clim.2008.07.017
Meloni, Francesca ; Accapezzato, Daniele ; Agresti, Cristina ; Aloisi, Francesca ; Ristori, Giovanni ; Salvetti, Marco ; Furlan, Roberto ; Martino, Gianvito ; Barnaba, Vincenzo ; Paroli, Marino. / Dendritic cells loaded with apoptotic oligodendrocytes as a source of myelin T-cell epitopes in multiple sclerosis. In: Clinical Immunology. 2008 ; Vol. 129, No. 2. pp. 286-294.
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