Dendritic cells modifi cation during sublingual immunotherapy in children with allergic symptoms to house dust mites

Federica Angelini, Valentina Pacciani, Stefania Corrente, Romina Silenzi, Alessandra Di Pede, Antonella Polito, Carla Riccardi, Silvia Di Cesare, Marie Laure Yammine, Paolo Rossi, Viviana Moschese, Loredana Chini

Research output: Contribution to journalArticle

Abstract

Background: The importance of dendritic cells (DCs) in the initiation of the Th2-mediated inflammatory response to allergens is well known and more recently it has been proposed that DCs have a pivotal role in maintaining tolerance to allergens. The aim of this study was to investigate whether the success of sublingual immunotherapy (SLIT) in allergic asthma is mediated by the induction of changes of DCs functions. Methods: Ten children with allergic asthma sensitive to house dust mite were studied before and after 12 months of SLIT. Immature DCs were derived from peripheral blood monocytes cultured for 6 days in presence of interleukin (IL)-4 and GM-CSF and stimulated with lipopolysaccharide for the last 24 hours to induce maturation. Results: After 12 months of SLIT, mature DCs derived from SLIT-treated patients showed a statistically significant defect of CD86 up-regulation, an increase of IL-10, and a reduction of IL-12 production. Conclusion: SLIT induces changes in DCs functions that might be responsible for an impairment of T cell activation or drive T cells towards a regulatory activity, thus restoring immune tolerance to allergens.

Original languageEnglish
Pages (from-to)24-30
Number of pages7
JournalWorld Journal of Pediatrics
Volume7
Issue number1
DOIs
Publication statusPublished - Feb 2011

Fingerprint

Sublingual Immunotherapy
Pyroglyphidae
Dendritic Cells
Cations
Allergens
Asthma
T-Lymphocytes
Immune Tolerance
Interleukin-12
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-4
Interleukin-10
Lipopolysaccharides
Monocytes
Up-Regulation

Keywords

  • allergy
  • CD86
  • dendritic cells
  • IL-10
  • sublingual immunotherapy

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Medicine(all)

Cite this

Dendritic cells modifi cation during sublingual immunotherapy in children with allergic symptoms to house dust mites. / Angelini, Federica; Pacciani, Valentina; Corrente, Stefania; Silenzi, Romina; Di Pede, Alessandra; Polito, Antonella; Riccardi, Carla; Di Cesare, Silvia; Yammine, Marie Laure; Rossi, Paolo; Moschese, Viviana; Chini, Loredana.

In: World Journal of Pediatrics, Vol. 7, No. 1, 02.2011, p. 24-30.

Research output: Contribution to journalArticle

Angelini, F, Pacciani, V, Corrente, S, Silenzi, R, Di Pede, A, Polito, A, Riccardi, C, Di Cesare, S, Yammine, ML, Rossi, P, Moschese, V & Chini, L 2011, 'Dendritic cells modifi cation during sublingual immunotherapy in children with allergic symptoms to house dust mites', World Journal of Pediatrics, vol. 7, no. 1, pp. 24-30. https://doi.org/10.1007/s12519-011-0242-3
Angelini, Federica ; Pacciani, Valentina ; Corrente, Stefania ; Silenzi, Romina ; Di Pede, Alessandra ; Polito, Antonella ; Riccardi, Carla ; Di Cesare, Silvia ; Yammine, Marie Laure ; Rossi, Paolo ; Moschese, Viviana ; Chini, Loredana. / Dendritic cells modifi cation during sublingual immunotherapy in children with allergic symptoms to house dust mites. In: World Journal of Pediatrics. 2011 ; Vol. 7, No. 1. pp. 24-30.
@article{8cef6338e1944e9999360beedb4f1955,
title = "Dendritic cells modifi cation during sublingual immunotherapy in children with allergic symptoms to house dust mites",
abstract = "Background: The importance of dendritic cells (DCs) in the initiation of the Th2-mediated inflammatory response to allergens is well known and more recently it has been proposed that DCs have a pivotal role in maintaining tolerance to allergens. The aim of this study was to investigate whether the success of sublingual immunotherapy (SLIT) in allergic asthma is mediated by the induction of changes of DCs functions. Methods: Ten children with allergic asthma sensitive to house dust mite were studied before and after 12 months of SLIT. Immature DCs were derived from peripheral blood monocytes cultured for 6 days in presence of interleukin (IL)-4 and GM-CSF and stimulated with lipopolysaccharide for the last 24 hours to induce maturation. Results: After 12 months of SLIT, mature DCs derived from SLIT-treated patients showed a statistically significant defect of CD86 up-regulation, an increase of IL-10, and a reduction of IL-12 production. Conclusion: SLIT induces changes in DCs functions that might be responsible for an impairment of T cell activation or drive T cells towards a regulatory activity, thus restoring immune tolerance to allergens.",
keywords = "allergy, CD86, dendritic cells, IL-10, sublingual immunotherapy",
author = "Federica Angelini and Valentina Pacciani and Stefania Corrente and Romina Silenzi and {Di Pede}, Alessandra and Antonella Polito and Carla Riccardi and {Di Cesare}, Silvia and Yammine, {Marie Laure} and Paolo Rossi and Viviana Moschese and Loredana Chini",
year = "2011",
month = "2",
doi = "10.1007/s12519-011-0242-3",
language = "English",
volume = "7",
pages = "24--30",
journal = "World Journal of Pediatrics",
issn = "1708-8569",
publisher = "Institute of Pediatrics of Zhejiang University",
number = "1",

}

TY - JOUR

T1 - Dendritic cells modifi cation during sublingual immunotherapy in children with allergic symptoms to house dust mites

AU - Angelini, Federica

AU - Pacciani, Valentina

AU - Corrente, Stefania

AU - Silenzi, Romina

AU - Di Pede, Alessandra

AU - Polito, Antonella

AU - Riccardi, Carla

AU - Di Cesare, Silvia

AU - Yammine, Marie Laure

AU - Rossi, Paolo

AU - Moschese, Viviana

AU - Chini, Loredana

PY - 2011/2

Y1 - 2011/2

N2 - Background: The importance of dendritic cells (DCs) in the initiation of the Th2-mediated inflammatory response to allergens is well known and more recently it has been proposed that DCs have a pivotal role in maintaining tolerance to allergens. The aim of this study was to investigate whether the success of sublingual immunotherapy (SLIT) in allergic asthma is mediated by the induction of changes of DCs functions. Methods: Ten children with allergic asthma sensitive to house dust mite were studied before and after 12 months of SLIT. Immature DCs were derived from peripheral blood monocytes cultured for 6 days in presence of interleukin (IL)-4 and GM-CSF and stimulated with lipopolysaccharide for the last 24 hours to induce maturation. Results: After 12 months of SLIT, mature DCs derived from SLIT-treated patients showed a statistically significant defect of CD86 up-regulation, an increase of IL-10, and a reduction of IL-12 production. Conclusion: SLIT induces changes in DCs functions that might be responsible for an impairment of T cell activation or drive T cells towards a regulatory activity, thus restoring immune tolerance to allergens.

AB - Background: The importance of dendritic cells (DCs) in the initiation of the Th2-mediated inflammatory response to allergens is well known and more recently it has been proposed that DCs have a pivotal role in maintaining tolerance to allergens. The aim of this study was to investigate whether the success of sublingual immunotherapy (SLIT) in allergic asthma is mediated by the induction of changes of DCs functions. Methods: Ten children with allergic asthma sensitive to house dust mite were studied before and after 12 months of SLIT. Immature DCs were derived from peripheral blood monocytes cultured for 6 days in presence of interleukin (IL)-4 and GM-CSF and stimulated with lipopolysaccharide for the last 24 hours to induce maturation. Results: After 12 months of SLIT, mature DCs derived from SLIT-treated patients showed a statistically significant defect of CD86 up-regulation, an increase of IL-10, and a reduction of IL-12 production. Conclusion: SLIT induces changes in DCs functions that might be responsible for an impairment of T cell activation or drive T cells towards a regulatory activity, thus restoring immune tolerance to allergens.

KW - allergy

KW - CD86

KW - dendritic cells

KW - IL-10

KW - sublingual immunotherapy

UR - http://www.scopus.com/inward/record.url?scp=79251591030&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79251591030&partnerID=8YFLogxK

U2 - 10.1007/s12519-011-0242-3

DO - 10.1007/s12519-011-0242-3

M3 - Article

C2 - 21191773

AN - SCOPUS:79251591030

VL - 7

SP - 24

EP - 30

JO - World Journal of Pediatrics

JF - World Journal of Pediatrics

SN - 1708-8569

IS - 1

ER -