Dendritic Cells Modulate Iron Homeostasis and Inflammatory Abilities Following Quercetin Exposure

Vanessa Galleggiante, Stefania De Santis, Elisabetta Cavalcanti, Aurelia Scarano, Maria De Benedictis, Grazia Serino, Maria Lucia Caruso, Mauro Mastronardi, Aldo Pinto, Pietro Campiglia, Dale Kunde, Angelo Santino, Marcello Chieppa

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

BACKGROUND: Fruits and vegetables are rich in plant polyphenols, whose consumption is encouraged in healthy dietary regimes due to their antioxidants and anti-inflammatory effects. These organic molecules exhibit numerous properties including phylochelation; the ability to complex metal ions, including highly reactive iron. Among polyphenols, we focused our attention on quercetin that previously demonstrated its ability to reduce dendritic cells (DCs) inflammatory cytokine secretion and antigen presentation following LPS exposure. Dendritic cell inflammatory response is also associated with modulation of several iron metabolism related genes.

OBJECTIVE: To characterize the axis between quercetin exposure and iron extracellular transport that may explain polyphenol anti-inflammatory abilities.

METHOD: Bone marrow derived DCs were exposed to 25μM of quercetin on day 7 and treated with 1 μg/mL of LPS on day 8. The relation between quercetin exposure and the expression level of genes involved in iron homeostasis was addressed by qPCR. The axis between iron export and quercetin exposure was confirmed in vitro and in vivo using quercetin gavage and quercetin-enriched diet.

RESULTS: Here we demonstrate that DCs, exposed to quercetin, activate a pattern of genes that increase extracellular iron export, resulting in an overall decrease in the intracellular iron content and consequent diminished inflammatory abilities. This DCs phenotype is consistent with anti-inflammatory phenotype of the mucosal resident DCs, the ones most commonly exposed to polyphenols.

CONCLUSIONS: Iron balance is a crucial checkpoint for DCs inflammatory abilities. Quercetin-enriched nutritional regimes that favor DCs extracellular iron transport could reduce the incidence of chronic inflammatory syndromes.

Original languageEnglish
Pages (from-to)2139-2146
Number of pages8
JournalCurrent Pharmaceutical Design
Volume23
Issue number14
DOIs
Publication statusPublished - 2017

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Quercetin
Dendritic Cells
Homeostasis
Iron
Polyphenols
Anti-Inflammatory Agents
Phenotype
Coordination Complexes
Antigen Presentation
Vegetables
Genes
Fruit
Antioxidants
Bone Marrow
Ions
Cytokines
Diet
Gene Expression
Incidence

Keywords

  • Journal Article

Cite this

Dendritic Cells Modulate Iron Homeostasis and Inflammatory Abilities Following Quercetin Exposure. / Galleggiante, Vanessa; De Santis, Stefania; Cavalcanti, Elisabetta; Scarano, Aurelia; De Benedictis, Maria; Serino, Grazia; Caruso, Maria Lucia; Mastronardi, Mauro; Pinto, Aldo; Campiglia, Pietro; Kunde, Dale; Santino, Angelo; Chieppa, Marcello.

In: Current Pharmaceutical Design, Vol. 23, No. 14, 2017, p. 2139-2146.

Research output: Contribution to journalArticle

Galleggiante, V, De Santis, S, Cavalcanti, E, Scarano, A, De Benedictis, M, Serino, G, Caruso, ML, Mastronardi, M, Pinto, A, Campiglia, P, Kunde, D, Santino, A & Chieppa, M 2017, 'Dendritic Cells Modulate Iron Homeostasis and Inflammatory Abilities Following Quercetin Exposure', Current Pharmaceutical Design, vol. 23, no. 14, pp. 2139-2146. https://doi.org/10.2174/1381612823666170112125355
Galleggiante, Vanessa ; De Santis, Stefania ; Cavalcanti, Elisabetta ; Scarano, Aurelia ; De Benedictis, Maria ; Serino, Grazia ; Caruso, Maria Lucia ; Mastronardi, Mauro ; Pinto, Aldo ; Campiglia, Pietro ; Kunde, Dale ; Santino, Angelo ; Chieppa, Marcello. / Dendritic Cells Modulate Iron Homeostasis and Inflammatory Abilities Following Quercetin Exposure. In: Current Pharmaceutical Design. 2017 ; Vol. 23, No. 14. pp. 2139-2146.
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T1 - Dendritic Cells Modulate Iron Homeostasis and Inflammatory Abilities Following Quercetin Exposure

AU - Galleggiante, Vanessa

AU - De Santis, Stefania

AU - Cavalcanti, Elisabetta

AU - Scarano, Aurelia

AU - De Benedictis, Maria

AU - Serino, Grazia

AU - Caruso, Maria Lucia

AU - Mastronardi, Mauro

AU - Pinto, Aldo

AU - Campiglia, Pietro

AU - Kunde, Dale

AU - Santino, Angelo

AU - Chieppa, Marcello

N1 - Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

PY - 2017

Y1 - 2017

N2 - BACKGROUND: Fruits and vegetables are rich in plant polyphenols, whose consumption is encouraged in healthy dietary regimes due to their antioxidants and anti-inflammatory effects. These organic molecules exhibit numerous properties including phylochelation; the ability to complex metal ions, including highly reactive iron. Among polyphenols, we focused our attention on quercetin that previously demonstrated its ability to reduce dendritic cells (DCs) inflammatory cytokine secretion and antigen presentation following LPS exposure. Dendritic cell inflammatory response is also associated with modulation of several iron metabolism related genes.OBJECTIVE: To characterize the axis between quercetin exposure and iron extracellular transport that may explain polyphenol anti-inflammatory abilities.METHOD: Bone marrow derived DCs were exposed to 25μM of quercetin on day 7 and treated with 1 μg/mL of LPS on day 8. The relation between quercetin exposure and the expression level of genes involved in iron homeostasis was addressed by qPCR. The axis between iron export and quercetin exposure was confirmed in vitro and in vivo using quercetin gavage and quercetin-enriched diet.RESULTS: Here we demonstrate that DCs, exposed to quercetin, activate a pattern of genes that increase extracellular iron export, resulting in an overall decrease in the intracellular iron content and consequent diminished inflammatory abilities. This DCs phenotype is consistent with anti-inflammatory phenotype of the mucosal resident DCs, the ones most commonly exposed to polyphenols.CONCLUSIONS: Iron balance is a crucial checkpoint for DCs inflammatory abilities. Quercetin-enriched nutritional regimes that favor DCs extracellular iron transport could reduce the incidence of chronic inflammatory syndromes.

AB - BACKGROUND: Fruits and vegetables are rich in plant polyphenols, whose consumption is encouraged in healthy dietary regimes due to their antioxidants and anti-inflammatory effects. These organic molecules exhibit numerous properties including phylochelation; the ability to complex metal ions, including highly reactive iron. Among polyphenols, we focused our attention on quercetin that previously demonstrated its ability to reduce dendritic cells (DCs) inflammatory cytokine secretion and antigen presentation following LPS exposure. Dendritic cell inflammatory response is also associated with modulation of several iron metabolism related genes.OBJECTIVE: To characterize the axis between quercetin exposure and iron extracellular transport that may explain polyphenol anti-inflammatory abilities.METHOD: Bone marrow derived DCs were exposed to 25μM of quercetin on day 7 and treated with 1 μg/mL of LPS on day 8. The relation between quercetin exposure and the expression level of genes involved in iron homeostasis was addressed by qPCR. The axis between iron export and quercetin exposure was confirmed in vitro and in vivo using quercetin gavage and quercetin-enriched diet.RESULTS: Here we demonstrate that DCs, exposed to quercetin, activate a pattern of genes that increase extracellular iron export, resulting in an overall decrease in the intracellular iron content and consequent diminished inflammatory abilities. This DCs phenotype is consistent with anti-inflammatory phenotype of the mucosal resident DCs, the ones most commonly exposed to polyphenols.CONCLUSIONS: Iron balance is a crucial checkpoint for DCs inflammatory abilities. Quercetin-enriched nutritional regimes that favor DCs extracellular iron transport could reduce the incidence of chronic inflammatory syndromes.

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