Dendritic cells produce TSLP that limits the differentiation of Th17 cells, fosters Treg development, and protects against colitis

I. Spadoni, I. D. Iliev, G. Rossi, M. Rescigno

Research output: Contribution to journalArticle

Abstract

Thymic stromal lymphopoietin (TSLP) is produced by epithelial cells and keratinocytes, and is involved in immune homeostasis or inflammation. The mechanism through which TSLP regulates intestinal inflammation is unclear. Here, we report that mouse dendritic cells (DCs) express TSLP both in vitro and in vivo in response to Toll-like receptor ligation in a MyD88-dependent fashion. TSLP is produced by the CD103 + subset of tolerogenic gut DCs and is downregulated during experimental colitis. TSLP produced by DCs acts directly on T cells by reducing their capacity to produce interleukin (IL)-17 and fostering the development of Foxp3 T cells. Consistently, TSLP protects against colitis development through a direct action on T cells, as adoptive transfer of nave T cells from TSLPR / to SCID mice results in a more severe colitis, with increased frequency of IL-17-producing T cells and inflammatory cytokines. Hence, we describe a new anti-inflammatory role of TSLP in the gut. 2012 Society for Mucosal Immunology.

Original languageEnglish
Pages (from-to)184-193
Number of pages10
JournalMucosal Immunology
Volume5
Issue number2
DOIs
Publication statusPublished - Mar 2012

Fingerprint

Th17 Cells
Colitis
Dendritic Cells
T-Lymphocytes
Interleukin-17
Inflammation
Foster Home Care
SCID Mice
Adoptive Transfer
Toll-Like Receptors
thymic stromal lymphopoietin
Allergy and Immunology
Keratinocytes
Ligation
Homeostasis
Anti-Inflammatory Agents
Down-Regulation
Epithelial Cells
Cytokines

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Dendritic cells produce TSLP that limits the differentiation of Th17 cells, fosters Treg development, and protects against colitis. / Spadoni, I.; Iliev, I. D.; Rossi, G.; Rescigno, M.

In: Mucosal Immunology, Vol. 5, No. 2, 03.2012, p. 184-193.

Research output: Contribution to journalArticle

@article{3507680841464c23a1f80ee5a63108db,
title = "Dendritic cells produce TSLP that limits the differentiation of Th17 cells, fosters Treg development, and protects against colitis",
abstract = "Thymic stromal lymphopoietin (TSLP) is produced by epithelial cells and keratinocytes, and is involved in immune homeostasis or inflammation. The mechanism through which TSLP regulates intestinal inflammation is unclear. Here, we report that mouse dendritic cells (DCs) express TSLP both in vitro and in vivo in response to Toll-like receptor ligation in a MyD88-dependent fashion. TSLP is produced by the CD103 + subset of tolerogenic gut DCs and is downregulated during experimental colitis. TSLP produced by DCs acts directly on T cells by reducing their capacity to produce interleukin (IL)-17 and fostering the development of Foxp3 T cells. Consistently, TSLP protects against colitis development through a direct action on T cells, as adoptive transfer of nave T cells from TSLPR / to SCID mice results in a more severe colitis, with increased frequency of IL-17-producing T cells and inflammatory cytokines. Hence, we describe a new anti-inflammatory role of TSLP in the gut. 2012 Society for Mucosal Immunology.",
author = "I. Spadoni and Iliev, {I. D.} and G. Rossi and M. Rescigno",
year = "2012",
month = "3",
doi = "10.1038/mi.2011.64",
language = "English",
volume = "5",
pages = "184--193",
journal = "Mucosal Immunology",
issn = "1933-0219",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - Dendritic cells produce TSLP that limits the differentiation of Th17 cells, fosters Treg development, and protects against colitis

AU - Spadoni, I.

AU - Iliev, I. D.

AU - Rossi, G.

AU - Rescigno, M.

PY - 2012/3

Y1 - 2012/3

N2 - Thymic stromal lymphopoietin (TSLP) is produced by epithelial cells and keratinocytes, and is involved in immune homeostasis or inflammation. The mechanism through which TSLP regulates intestinal inflammation is unclear. Here, we report that mouse dendritic cells (DCs) express TSLP both in vitro and in vivo in response to Toll-like receptor ligation in a MyD88-dependent fashion. TSLP is produced by the CD103 + subset of tolerogenic gut DCs and is downregulated during experimental colitis. TSLP produced by DCs acts directly on T cells by reducing their capacity to produce interleukin (IL)-17 and fostering the development of Foxp3 T cells. Consistently, TSLP protects against colitis development through a direct action on T cells, as adoptive transfer of nave T cells from TSLPR / to SCID mice results in a more severe colitis, with increased frequency of IL-17-producing T cells and inflammatory cytokines. Hence, we describe a new anti-inflammatory role of TSLP in the gut. 2012 Society for Mucosal Immunology.

AB - Thymic stromal lymphopoietin (TSLP) is produced by epithelial cells and keratinocytes, and is involved in immune homeostasis or inflammation. The mechanism through which TSLP regulates intestinal inflammation is unclear. Here, we report that mouse dendritic cells (DCs) express TSLP both in vitro and in vivo in response to Toll-like receptor ligation in a MyD88-dependent fashion. TSLP is produced by the CD103 + subset of tolerogenic gut DCs and is downregulated during experimental colitis. TSLP produced by DCs acts directly on T cells by reducing their capacity to produce interleukin (IL)-17 and fostering the development of Foxp3 T cells. Consistently, TSLP protects against colitis development through a direct action on T cells, as adoptive transfer of nave T cells from TSLPR / to SCID mice results in a more severe colitis, with increased frequency of IL-17-producing T cells and inflammatory cytokines. Hence, we describe a new anti-inflammatory role of TSLP in the gut. 2012 Society for Mucosal Immunology.

UR - http://www.scopus.com/inward/record.url?scp=84857221390&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84857221390&partnerID=8YFLogxK

U2 - 10.1038/mi.2011.64

DO - 10.1038/mi.2011.64

M3 - Article

C2 - 22236997

AN - SCOPUS:84857221390

VL - 5

SP - 184

EP - 193

JO - Mucosal Immunology

JF - Mucosal Immunology

SN - 1933-0219

IS - 2

ER -