Abstract
Densitometry, a classical cytophotometric technique that quantitatively evaluates DNA content of cell nuclei, has been renewed through introduction of electronic technology; thus, we have chosen it to study larqe plaque parapsoriasis in prognostical terms. Six patients affected with a slowly progressing form of the disease were found to possess a normal diploid genome. In contrast, 2 surely positive neoplastic controls (1 aggressive form of mycosis fungoides; 1 lymphomatous erythroderma) showed tetraploidy and/or an increased S-phase cell fraction: this could mean active proliferation. This technique is simple, and does spare sample entireness (differently from laser flow cytometry). According to results given by other Authors, densitometry could be proposed as an interesting method to differentiate 'benign' parapsoriasis from insidious mycosis fungoides. Besides, sample preparation is easy (traditional Feulgen-Rossenbeck coloration, as modified by Pearse), within the reach of every equipe working together with an histopathology laboratory. Anyway, we must remark that it is necessary to evaluate critically densitometric results, and to integrate them within a wider context including clinical and other laboratory data. Only under these conditions, densitometry will be able to become an interesting method helping to define prognosis of puzzling lymphoproliferative diseases.
| Original language | Italian |
|---|---|
| Pages (from-to) | 233-238 |
| Number of pages | 6 |
| Journal | Annali Italiani di Dermatologia Clinica e Sperimentale |
| Volume | 45 |
| Issue number | 4 |
| Publication status | Published - 1991 |
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ASJC Scopus subject areas
- Dermatology
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LA DENSITOMETRIA NELLO STUDIO DELLA PARAPSORIASI IN CHIAZZE. / Altomare, G.; Capella, G. L.; Pigatto, P.; Finzi, A. F.; Biondo, B.; Lavezzi, A. M.; Matturri, L.
In: Annali Italiani di Dermatologia Clinica e Sperimentale, Vol. 45, No. 4, 1991, p. 233-238.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - LA DENSITOMETRIA NELLO STUDIO DELLA PARAPSORIASI IN CHIAZZE
AU - Altomare, G.
AU - Capella, G. L.
AU - Pigatto, P.
AU - Finzi, A. F.
AU - Biondo, B.
AU - Lavezzi, A. M.
AU - Matturri, L.
PY - 1991
Y1 - 1991
N2 - Densitometry, a classical cytophotometric technique that quantitatively evaluates DNA content of cell nuclei, has been renewed through introduction of electronic technology; thus, we have chosen it to study larqe plaque parapsoriasis in prognostical terms. Six patients affected with a slowly progressing form of the disease were found to possess a normal diploid genome. In contrast, 2 surely positive neoplastic controls (1 aggressive form of mycosis fungoides; 1 lymphomatous erythroderma) showed tetraploidy and/or an increased S-phase cell fraction: this could mean active proliferation. This technique is simple, and does spare sample entireness (differently from laser flow cytometry). According to results given by other Authors, densitometry could be proposed as an interesting method to differentiate 'benign' parapsoriasis from insidious mycosis fungoides. Besides, sample preparation is easy (traditional Feulgen-Rossenbeck coloration, as modified by Pearse), within the reach of every equipe working together with an histopathology laboratory. Anyway, we must remark that it is necessary to evaluate critically densitometric results, and to integrate them within a wider context including clinical and other laboratory data. Only under these conditions, densitometry will be able to become an interesting method helping to define prognosis of puzzling lymphoproliferative diseases.
AB - Densitometry, a classical cytophotometric technique that quantitatively evaluates DNA content of cell nuclei, has been renewed through introduction of electronic technology; thus, we have chosen it to study larqe plaque parapsoriasis in prognostical terms. Six patients affected with a slowly progressing form of the disease were found to possess a normal diploid genome. In contrast, 2 surely positive neoplastic controls (1 aggressive form of mycosis fungoides; 1 lymphomatous erythroderma) showed tetraploidy and/or an increased S-phase cell fraction: this could mean active proliferation. This technique is simple, and does spare sample entireness (differently from laser flow cytometry). According to results given by other Authors, densitometry could be proposed as an interesting method to differentiate 'benign' parapsoriasis from insidious mycosis fungoides. Besides, sample preparation is easy (traditional Feulgen-Rossenbeck coloration, as modified by Pearse), within the reach of every equipe working together with an histopathology laboratory. Anyway, we must remark that it is necessary to evaluate critically densitometric results, and to integrate them within a wider context including clinical and other laboratory data. Only under these conditions, densitometry will be able to become an interesting method helping to define prognosis of puzzling lymphoproliferative diseases.
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M3 - Articolo
VL - 45
SP - 233
EP - 238
JO - Annali Italiani di Dermatologia Allergologica Clinica e Sperimentale
JF - Annali Italiani di Dermatologia Allergologica Clinica e Sperimentale
SN - 1592-6826
IS - 4
ER -