Dentifrice containing extract of Rosmarinus officinalis Linn. An antimicrobial evaluation

Marcela Agne Alves Valones, Jane Sheila Higino, Paulo Roberto Eleutério Souza, Sérgio Crovella, Arnaldo de França Caldas Júnior, Alessandra de Albuquerque Tavares Carvalho

Research output: Contribution to journalArticle


This study aimed to evaluate the antimicrobial activity of a dentifrice containing an alcoholic extract of rosemary on oral bacteria, compared to a commercially available herbal dentifrice. Standard strains of Streptococcus mutans (ATCC 25175), Streptococcus oralis (ATCC 9811) and Lactobacillus rhamnosus (ATCC 7469) were used, as well as different toothpastes based on rosemary (TR), on propolis (TH), triclosan (positive control) (TPC) and non-fluoridated dentifrice (negative control) (TNC). Bacteria were seeded in Petri dishes and paper discs soaked with dilutions of dentifrice placed on the plates. The inhibition halos were analyzed. It was observed that TR did not show statistical difference in relation to the TH to inhibit S. mutans and S. oralis, while TH was more active against L. rhamnosus. The toothpaste containing rosemary extract had the ability to inhibit the growth of S. mutans, S. oralis and L. rhamnosus, revealing an antimicrobial activity similar to commercially available toothpastes for inhibition of S. mutans and S. oralis.

Original languageEnglish
Pages (from-to)497-501
Number of pages5
JournalBrazilian Dental Journal
Issue number5
Publication statusPublished - Sep 1 2016


  • Antimicrobial activity
  • Dentifrice
  • Phytotherapy
  • Rosmarinus officinalis

ASJC Scopus subject areas

  • Dentistry(all)

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  • Cite this

    Valones, M. A. A., Higino, J. S., Souza, P. R. E., Crovella, S., Júnior, A. D. F. C., & Carvalho, A. D. A. T. (2016). Dentifrice containing extract of Rosmarinus officinalis Linn. An antimicrobial evaluation. Brazilian Dental Journal, 27(5), 497-501.