Depletion of circulating allergen-specific T(H2) T lymphocytes after allergen exposure in asthma

E. Crimi, D. Gaffi, E. Frittoli, B. Borgonovo, S. E. Burastero

Research output: Contribution to journalArticlepeer-review


Background: In allergic asthma, CD4 + T lymphocytes are a fundamental component of local chronic inflammation. Their cytokine profile is oriented toward a T(H2) phenotype, characterized by production of IL-4, IL-5, IL-10, and IL-13. Egress of T cells from blood to airways after allergen challenge has been described. Objective: We have studied a cohort of six patients with asthma who had multiple allergies to investigate how exposure to allergen affects the proliferation of peripheral CD4 + T lymphocytes with different allergen specificities and lymphokine profiles. Methods: For each patient, CD4 + T-cell lines were generated by in vitro stimulation with sensitizing and with nonsensitizing allergens, and IL-4 and interferon-γ production by these lines was assessed. Proliferation of peripheral blood CD4 + T lymphocytes in response to the same allergens was measured before and 24 hours after inhalation challenge with a sensitizing allergen. Results: We found that each single sensitizing allergen can deplete peripheral blood of T(H2)-type CD4 + T lymphocytes specific for all sensitizing allergens, but not of T(H2)-type CD4 + T lymphocytes. Conclusions: Our results suggest the existence of mechanisms capable of sorting disease-associated antigen specificities together with defined lymphokine patterns into T lymphocytes that can migrate to target organs, in allergic asthma.

Original languageEnglish
Pages (from-to)788-797
Number of pages10
JournalJournal of Allergy and Clinical Immunology
Issue number6 I SUPPL.
Publication statusPublished - 1997


  • Allergy
  • Asthma
  • Bronchial provocation tests
  • T(H2) lymphocytes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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