TY - JOUR
T1 - Depletion of circulating allergen-specific T(H2) T lymphocytes after allergen exposure in asthma
AU - Crimi, E.
AU - Gaffi, D.
AU - Frittoli, E.
AU - Borgonovo, B.
AU - Burastero, S. E.
PY - 1997
Y1 - 1997
N2 - Background: In allergic asthma, CD4
+ T lymphocytes are a fundamental component of local chronic inflammation. Their cytokine profile is oriented toward a T(H2) phenotype, characterized by production of IL-4, IL-5, IL-10, and IL-13. Egress of T cells from blood to airways after allergen challenge has been described. Objective: We have studied a cohort of six patients with asthma who had multiple allergies to investigate how exposure to allergen affects the proliferation of peripheral CD4
+ T lymphocytes with different allergen specificities and lymphokine profiles. Methods: For each patient, CD4
+ T-cell lines were generated by in vitro stimulation with sensitizing and with nonsensitizing allergens, and IL-4 and interferon-γ production by these lines was assessed. Proliferation of peripheral blood CD4
+ T lymphocytes in response to the same allergens was measured before and 24 hours after inhalation challenge with a sensitizing allergen. Results: We found that each single sensitizing allergen can deplete peripheral blood of T(H2)-type CD4
+ T lymphocytes specific for all sensitizing allergens, but not of T(H2)-type CD4
+ T lymphocytes. Conclusions: Our results suggest the existence of mechanisms capable of sorting disease-associated antigen specificities together with defined lymphokine patterns into T lymphocytes that can migrate to target organs, in allergic asthma.
AB - Background: In allergic asthma, CD4
+ T lymphocytes are a fundamental component of local chronic inflammation. Their cytokine profile is oriented toward a T(H2) phenotype, characterized by production of IL-4, IL-5, IL-10, and IL-13. Egress of T cells from blood to airways after allergen challenge has been described. Objective: We have studied a cohort of six patients with asthma who had multiple allergies to investigate how exposure to allergen affects the proliferation of peripheral CD4
+ T lymphocytes with different allergen specificities and lymphokine profiles. Methods: For each patient, CD4
+ T-cell lines were generated by in vitro stimulation with sensitizing and with nonsensitizing allergens, and IL-4 and interferon-γ production by these lines was assessed. Proliferation of peripheral blood CD4
+ T lymphocytes in response to the same allergens was measured before and 24 hours after inhalation challenge with a sensitizing allergen. Results: We found that each single sensitizing allergen can deplete peripheral blood of T(H2)-type CD4
+ T lymphocytes specific for all sensitizing allergens, but not of T(H2)-type CD4
+ T lymphocytes. Conclusions: Our results suggest the existence of mechanisms capable of sorting disease-associated antigen specificities together with defined lymphokine patterns into T lymphocytes that can migrate to target organs, in allergic asthma.
KW - Allergy
KW - Asthma
KW - Bronchial provocation tests
KW - T(H2) lymphocytes
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U2 - 10.1016/S0091-6749(97)80013-9
DO - 10.1016/S0091-6749(97)80013-9
M3 - Article
C2 - 9215247
AN - SCOPUS:0030927431
VL - 99
SP - 788
EP - 797
JO - Journal of Allergy and Clinical Immunology
JF - Journal of Allergy and Clinical Immunology
SN - 0091-6749
IS - 6 I SUPPL.
ER -