Depletion of endonuclease G selectively kills polyploid cells

Sabrina Büttner, Didac Carmona-Gutierrez, Ilio Vitale, Maria Castedo, Doris Ruli, Tobias Eisenberg, Guido Kroemer, Frank Madeo

Research output: Contribution to journalArticle

Abstract

Endonuclease G is a mitochondrio-nuclear located nuclease with dual - vital and lethal - functions. Besides its role in apoptosis execution, we have recently shown that depletion of endonuclease G leads to necrotic cell death in yeast. Here, we present further mechanistic elucidation of endonuclease G's vital functions. The deletion of the yeast Endonuclease G gene causes the complete elimination of tetraploid cells during exponential growth. Consistently, conditional knockdown of mammalian endonuclease G selectively kills tetraploid but not diploid clones of the human HCT116 colon carcinoma cell line. We conclude that endonuclease G is important for the viability of polyploid mammalian and yeast cells.

Original languageEnglish
Pages (from-to)1072-1076
Number of pages5
JournalCell Cycle
Volume6
Issue number9
Publication statusPublished - May 1 2007

Keywords

  • Apoptosis
  • Cancer
  • Cell death
  • Endonuclease G
  • Polyploidy
  • Yeast

ASJC Scopus subject areas

  • Cell Biology
  • Biochemistry
  • Molecular Biology

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    Büttner, S., Carmona-Gutierrez, D., Vitale, I., Castedo, M., Ruli, D., Eisenberg, T., Kroemer, G., & Madeo, F. (2007). Depletion of endonuclease G selectively kills polyploid cells. Cell Cycle, 6(9), 1072-1076.