Deposition of chromosomal protein HMG-17 during replication affects the nucleosomal ladder and transcriptional potential of nascent chromatin

Massimo P. Crippa, Lothar Trieschmann, Pedro J. Alfonso, Alan P. Wolffe, Michael Bustin

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

A cell-free system from Xenopus eggs was used to study the role of chromosomal protein HMG-17 in the generation of the chromatin structure of transcriptionally active genes. Addition of HMG-17 protein to the extracts, which do not contain structural homologs of the HMG-14/-17 protein family, indicates the protein is incorporated into the nascent template during replication, prior to completion of chromatin assembly. The protein binds to and stabilizes the structure of the nucleosomal core thereby improving the apparent periodicity of the nucleosomal spacing of nascent chromatin. Assembly of HMG-17 into the nascent chromatin structure significantly increased the transcription potential of the 5S RNA gene and satellite I chromatin. Kinetic studies indicate that the increase in transcriptional potential is observed only when HMG-17 is incorporated into nucleosomes during chromatin assembly.

Original languageEnglish
Pages (from-to)3855-3864
Number of pages10
JournalEMBO Journal
Volume12
Issue number10
Publication statusPublished - 1993

Fingerprint

HMGN2 Protein
Ladders
Chromatin
Chromatin Assembly and Disassembly
HMGN1 Protein
Satellite RNAs
Genes
Cell-Free System
Nucleosomes
Periodicity
Xenopus
Proteins
Eggs
Transcription
Satellites
RNA
Kinetics

Keywords

  • Active chromatin
  • Chromatin assembly
  • Chromosomal proteins
  • Transcription
  • Xenopus

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Deposition of chromosomal protein HMG-17 during replication affects the nucleosomal ladder and transcriptional potential of nascent chromatin. / Crippa, Massimo P.; Trieschmann, Lothar; Alfonso, Pedro J.; Wolffe, Alan P.; Bustin, Michael.

In: EMBO Journal, Vol. 12, No. 10, 1993, p. 3855-3864.

Research output: Contribution to journalArticle

Crippa, Massimo P. ; Trieschmann, Lothar ; Alfonso, Pedro J. ; Wolffe, Alan P. ; Bustin, Michael. / Deposition of chromosomal protein HMG-17 during replication affects the nucleosomal ladder and transcriptional potential of nascent chromatin. In: EMBO Journal. 1993 ; Vol. 12, No. 10. pp. 3855-3864.
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AU - Bustin, Michael

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