TY - JOUR
T1 - Depression of liver metabolism and induction of cytokine release by diphtheria and tetanus toxoids and pertussis vaccines
T2 - Role of Bordetella pertussis cells in toxicity
AU - Fantuzzi, G.
AU - Sironi, M.
AU - Delgado, R.
AU - Cantoni, L.
AU - Rizzardini, M.
AU - Carelli, M.
AU - Marsili, I.
AU - Ghiara, P.
AU - Ghezzi, P.
PY - 1994
Y1 - 1994
N2 - A 24-h pretreatment of mice with diphtheria and tetanus toxoids and whole- cell pertussis vaccines depressed liver cytochrome P-450 and therefore prolonged hexobarbital-induced sleeping time in mice. The depression of liver drug metabolism by a cellular vaccine containing a mutated pertussis toxin was less marked than that induced by the wild-type vaccines, indicating that the mutated vaccine might have lower toxicity in this regard. The wild-type vaccines decreased microsomal P-450 levels by 50%, while the mutated whole- cell vaccine had a less marked effect (a decrease of 30%), paralleling the results obtained in sleeping time experiments. Furthermore, an acellular mutated vaccine did not affect liver drug metabolism, indicating a role of the whole bacterial cell in this side effect. All the cellular vaccines studied induced high serum interleukin-6 levels; on the other hand, the acellular mutated vaccine induced very low interleukin-6 levels, indicating that the whole bacterial cell is also important for interleukin-6 induction. All vaccines studied were very poor tumor necrosis factor inducers.
AB - A 24-h pretreatment of mice with diphtheria and tetanus toxoids and whole- cell pertussis vaccines depressed liver cytochrome P-450 and therefore prolonged hexobarbital-induced sleeping time in mice. The depression of liver drug metabolism by a cellular vaccine containing a mutated pertussis toxin was less marked than that induced by the wild-type vaccines, indicating that the mutated vaccine might have lower toxicity in this regard. The wild-type vaccines decreased microsomal P-450 levels by 50%, while the mutated whole- cell vaccine had a less marked effect (a decrease of 30%), paralleling the results obtained in sleeping time experiments. Furthermore, an acellular mutated vaccine did not affect liver drug metabolism, indicating a role of the whole bacterial cell in this side effect. All the cellular vaccines studied induced high serum interleukin-6 levels; on the other hand, the acellular mutated vaccine induced very low interleukin-6 levels, indicating that the whole bacterial cell is also important for interleukin-6 induction. All vaccines studied were very poor tumor necrosis factor inducers.
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M3 - Article
C2 - 8262641
AN - SCOPUS:0028029139
VL - 62
SP - 29
EP - 32
JO - Infection and Immunity
JF - Infection and Immunity
SN - 0019-9567
IS - 1
ER -