Depression of liver metabolism and induction of cytokine release by diphtheria and tetanus toxoids and pertussis vaccines: Role of Bordetella pertussis cells in toxicity

G. Fantuzzi, M. Sironi, R. Delgado, L. Cantoni, M. Rizzardini, M. Carelli, I. Marsili, P. Ghiara, P. Ghezzi

Research output: Contribution to journalArticlepeer-review

Abstract

A 24-h pretreatment of mice with diphtheria and tetanus toxoids and whole- cell pertussis vaccines depressed liver cytochrome P-450 and therefore prolonged hexobarbital-induced sleeping time in mice. The depression of liver drug metabolism by a cellular vaccine containing a mutated pertussis toxin was less marked than that induced by the wild-type vaccines, indicating that the mutated vaccine might have lower toxicity in this regard. The wild-type vaccines decreased microsomal P-450 levels by 50%, while the mutated whole- cell vaccine had a less marked effect (a decrease of 30%), paralleling the results obtained in sleeping time experiments. Furthermore, an acellular mutated vaccine did not affect liver drug metabolism, indicating a role of the whole bacterial cell in this side effect. All the cellular vaccines studied induced high serum interleukin-6 levels; on the other hand, the acellular mutated vaccine induced very low interleukin-6 levels, indicating that the whole bacterial cell is also important for interleukin-6 induction. All vaccines studied were very poor tumor necrosis factor inducers.

Original languageEnglish
Pages (from-to)29-32
Number of pages4
JournalInfection and Immunity
Volume62
Issue number1
Publication statusPublished - 1994

ASJC Scopus subject areas

  • Immunology

Fingerprint Dive into the research topics of 'Depression of liver metabolism and induction of cytokine release by diphtheria and tetanus toxoids and pertussis vaccines: Role of Bordetella pertussis cells in toxicity'. Together they form a unique fingerprint.

Cite this