Deregulation of Aurora kinase gene expression in human testicular germ cell tumours

E. Baldini, Y. Arlot-Bonnemains, M. Mottolese, S. Sentinelli, B. Antoniani, S. Sorrenti, M. Salducci, E. Comini, S. Ulisse, M. D'Armiento

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The Aurora kinases regulate chromosome segregation and cytokinesis, and alterations in their expression associate with cell malignant transformation. In this study, we demonstrated by qRT-PCR analysis of 14 seminomas that Aurora-A mRNA was, with respect to control tissues, augmented in five of 14 tumour tissues by 2.17 ± 0.30 fold (P <0.05) and reduced in 9 to 0.38 ± 0.10 (P <0.01). Aurora-B mRNA was increased in 11 tumour tissues by 4.33 ± 0.82 fold (P <0.01) and reduced in 3 to 0.41 ± 0.11 fold. Aurora-C mRNA was reduced to 0.20 ± 0.32 fold (P <0.01) in 13 seminomas and up-regulated in one case. Western blot experiments, performed on protein extracts of nine seminomas and six normal testes, showed an up-regulation of Aurora-B protein by 10.14 ± 3.51 fold (P <0.05), while Aurora-A protein was found increased in four seminomas by 2.16 ± 0.43 (P <0.05), unchanged in three and reduced in two tumour tissues. Aurora-C protein was increased by 9.2 ± 2.90 fold (P <0.05), suggesting that post-transcriptional mechanisms modulate its expression. In conclusion, we demonstrated that expression of Aurora kinases is deregulated in seminomas, suggesting that they may play a role in the progression of testicular cancers.

Original languageEnglish
Pages (from-to)260-267
Number of pages8
JournalAndrologia
Volume42
Issue number4
DOIs
Publication statusPublished - 2010

Fingerprint

Aurora Kinases
Seminoma
Gene Expression
Messenger RNA
Neoplasms
Chromosome Segregation
Cytokinesis
Testicular Neoplasms
Protein C
Testis
Proteins
Up-Regulation
Western Blotting
Testicular Germ Cell Tumor
Polymerase Chain Reaction

Keywords

  • Aneuploidy
  • Aurora kinases
  • genomic instability
  • mitosis
  • testicular germ cell tumour

ASJC Scopus subject areas

  • Urology
  • Endocrinology
  • Medicine(all)

Cite this

Baldini, E., Arlot-Bonnemains, Y., Mottolese, M., Sentinelli, S., Antoniani, B., Sorrenti, S., ... D'Armiento, M. (2010). Deregulation of Aurora kinase gene expression in human testicular germ cell tumours. Andrologia, 42(4), 260-267. https://doi.org/10.1111/j.1439-0272.2009.00987.x

Deregulation of Aurora kinase gene expression in human testicular germ cell tumours. / Baldini, E.; Arlot-Bonnemains, Y.; Mottolese, M.; Sentinelli, S.; Antoniani, B.; Sorrenti, S.; Salducci, M.; Comini, E.; Ulisse, S.; D'Armiento, M.

In: Andrologia, Vol. 42, No. 4, 2010, p. 260-267.

Research output: Contribution to journalArticle

Baldini, E, Arlot-Bonnemains, Y, Mottolese, M, Sentinelli, S, Antoniani, B, Sorrenti, S, Salducci, M, Comini, E, Ulisse, S & D'Armiento, M 2010, 'Deregulation of Aurora kinase gene expression in human testicular germ cell tumours', Andrologia, vol. 42, no. 4, pp. 260-267. https://doi.org/10.1111/j.1439-0272.2009.00987.x
Baldini, E. ; Arlot-Bonnemains, Y. ; Mottolese, M. ; Sentinelli, S. ; Antoniani, B. ; Sorrenti, S. ; Salducci, M. ; Comini, E. ; Ulisse, S. ; D'Armiento, M. / Deregulation of Aurora kinase gene expression in human testicular germ cell tumours. In: Andrologia. 2010 ; Vol. 42, No. 4. pp. 260-267.
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