Abstract
Background: The association of clinical and dermoscopic features with BRAF mutational status has been poorly analysed in multiple primary melanomas (MPM). Objective: To investigate whether concordance of BRAF mutational status is associated with dermoscopic similarity in multiple melanomas of the same patient. Methods: Dermoscopic images and corresponding tissue sections of 124 melanomas from 62 patients with MPM were selected at four Italian Dermatology Departments. Similarity of dermoscopic appearance between multiple melanomas was evaluated according to the presence of the same prevalent dermoscopic feature. The BRAF V600 mutational status was analysed with allele-specific TaqMan TM assays or pyrosequencing. Spearman's correlation and univariate and multivariate regression analysis were used for statistical analysis. Results: A similar dermoscopic appearance was identified in 38.7% (24/62) of patients with MPM and was correlated with older age at first diagnosis (rho: 0.26; P: 0.042) and occurrence on sun-damaged skin (rho: 0.27; P: 0.037). The BRAF V600 mutation was detected in 39.5% (49/124) of the tumors and a concordant BRAF mutational status between melanomas in 33/62 (53.2%) MPM patients. Dermoscopically similar melanomas showed 5.7-fold higher odds to be concordant for BRAF mutational status compared to dissimilar lesions (OR: 5.7; 95% CI 1.7-19.5; P: 0.005). Conclusion: Dermoscopic similarity of multiple melanomas represents an independent clinical predictor of a concordant BRAF mutational status in MPM patients.
| Original language | English |
|---|---|
| Journal | Experimental Dermatology |
| DOIs | |
| Publication status | Published - Jan 1 2019 |
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Keywords
- BRAF
- dermoscopy
- molecular analyses
- multiple primary melanomas
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Dermatology
Cite this
Dermoscopic similarity is an independent predictor of BRAF mutational concordance in multiple melanomas. / Moscarella, Elvira; Pellegrini, Cristina; Pampena, Riccardo; Argenziano, Giuseppe; Manfredini, Marco; Martorelli, Claudia; Ciarrocchi, Alessia; Dika, Emi; Peris, Ketty; Antonini, Ambra; Cipolloni, Gianluca; Alfano, Roberto; Longo, Caterina; Fargnoli, Maria Concetta.
In: Experimental Dermatology, 01.01.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dermoscopic similarity is an independent predictor of BRAF mutational concordance in multiple melanomas
AU - Moscarella, Elvira
AU - Pellegrini, Cristina
AU - Pampena, Riccardo
AU - Argenziano, Giuseppe
AU - Manfredini, Marco
AU - Martorelli, Claudia
AU - Ciarrocchi, Alessia
AU - Dika, Emi
AU - Peris, Ketty
AU - Antonini, Ambra
AU - Cipolloni, Gianluca
AU - Alfano, Roberto
AU - Longo, Caterina
AU - Fargnoli, Maria Concetta
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Background: The association of clinical and dermoscopic features with BRAF mutational status has been poorly analysed in multiple primary melanomas (MPM). Objective: To investigate whether concordance of BRAF mutational status is associated with dermoscopic similarity in multiple melanomas of the same patient. Methods: Dermoscopic images and corresponding tissue sections of 124 melanomas from 62 patients with MPM were selected at four Italian Dermatology Departments. Similarity of dermoscopic appearance between multiple melanomas was evaluated according to the presence of the same prevalent dermoscopic feature. The BRAF V600 mutational status was analysed with allele-specific TaqMan TM assays or pyrosequencing. Spearman's correlation and univariate and multivariate regression analysis were used for statistical analysis. Results: A similar dermoscopic appearance was identified in 38.7% (24/62) of patients with MPM and was correlated with older age at first diagnosis (rho: 0.26; P: 0.042) and occurrence on sun-damaged skin (rho: 0.27; P: 0.037). The BRAF V600 mutation was detected in 39.5% (49/124) of the tumors and a concordant BRAF mutational status between melanomas in 33/62 (53.2%) MPM patients. Dermoscopically similar melanomas showed 5.7-fold higher odds to be concordant for BRAF mutational status compared to dissimilar lesions (OR: 5.7; 95% CI 1.7-19.5; P: 0.005). Conclusion: Dermoscopic similarity of multiple melanomas represents an independent clinical predictor of a concordant BRAF mutational status in MPM patients.
AB - Background: The association of clinical and dermoscopic features with BRAF mutational status has been poorly analysed in multiple primary melanomas (MPM). Objective: To investigate whether concordance of BRAF mutational status is associated with dermoscopic similarity in multiple melanomas of the same patient. Methods: Dermoscopic images and corresponding tissue sections of 124 melanomas from 62 patients with MPM were selected at four Italian Dermatology Departments. Similarity of dermoscopic appearance between multiple melanomas was evaluated according to the presence of the same prevalent dermoscopic feature. The BRAF V600 mutational status was analysed with allele-specific TaqMan TM assays or pyrosequencing. Spearman's correlation and univariate and multivariate regression analysis were used for statistical analysis. Results: A similar dermoscopic appearance was identified in 38.7% (24/62) of patients with MPM and was correlated with older age at first diagnosis (rho: 0.26; P: 0.042) and occurrence on sun-damaged skin (rho: 0.27; P: 0.037). The BRAF V600 mutation was detected in 39.5% (49/124) of the tumors and a concordant BRAF mutational status between melanomas in 33/62 (53.2%) MPM patients. Dermoscopically similar melanomas showed 5.7-fold higher odds to be concordant for BRAF mutational status compared to dissimilar lesions (OR: 5.7; 95% CI 1.7-19.5; P: 0.005). Conclusion: Dermoscopic similarity of multiple melanomas represents an independent clinical predictor of a concordant BRAF mutational status in MPM patients.
KW - BRAF
KW - dermoscopy
KW - molecular analyses
KW - multiple primary melanomas
UR - http://www.scopus.com/inward/record.url?scp=85065703951&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85065703951&partnerID=8YFLogxK
U2 - 10.1111/exd.13951
DO - 10.1111/exd.13951
M3 - Article
C2 - 31034104
AN - SCOPUS:85065703951
JO - Experimental Dermatology
JF - Experimental Dermatology
SN - 0906-6705
ER -