Dermoscopic similarity is an independent predictor of BRAF mutational concordance in multiple melanomas

Elvira Moscarella, Cristina Pellegrini, Riccardo Pampena, Giuseppe Argenziano, Marco Manfredini, Claudia Martorelli, Alessia Ciarrocchi, Emi Dika, Ketty Peris, Ambra Antonini, Gianluca Cipolloni, Roberto Alfano, Caterina Longo, Maria Concetta Fargnoli

Research output: Contribution to journal › Article

Abstract

Background: The association of clinical and dermoscopic features with BRAF mutational status has been poorly analysed in multiple primary melanomas (MPM). Objective: To investigate whether concordance of BRAF mutational status is associated with dermoscopic similarity in multiple melanomas of the same patient. Methods: Dermoscopic images and corresponding tissue sections of 124 melanomas from 62 patients with MPM were selected at four Italian Dermatology Departments. Similarity of dermoscopic appearance between multiple melanomas was evaluated according to the presence of the same prevalent dermoscopic feature. The BRAF V600 mutational status was analysed with allele-specific TaqMan TM assays or pyrosequencing. Spearman's correlation and univariate and multivariate regression analysis were used for statistical analysis. Results: A similar dermoscopic appearance was identified in 38.7% (24/62) of patients with MPM and was correlated with older age at first diagnosis (rho: 0.26; P: 0.042) and occurrence on sun-damaged skin (rho: 0.27; P: 0.037). The BRAF V600 mutation was detected in 39.5% (49/124) of the tumors and a concordant BRAF mutational status between melanomas in 33/62 (53.2%) MPM patients. Dermoscopically similar melanomas showed 5.7-fold higher odds to be concordant for BRAF mutational status compared to dissimilar lesions (OR: 5.7; 95% CI 1.7-19.5; P: 0.005). Conclusion: Dermoscopic similarity of multiple melanomas represents an independent clinical predictor of a concordant BRAF mutational status in MPM patients.

Original languageEnglish
JournalExperimental Dermatology
DOIs
Publication statusPublished - Jan 1 2019

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Dermatology
Regression analysis
Sun
Tumors
Melanoma
Assays
Statistical methods
Skin
Association reactions
Tissue
Solar System

Keywords

  • BRAF
  • dermoscopy
  • molecular analyses
  • multiple primary melanomas

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

Moscarella, E., Pellegrini, C., Pampena, R., Argenziano, G., Manfredini, M., Martorelli, C., ... Fargnoli, M. C. (2019). Dermoscopic similarity is an independent predictor of BRAF mutational concordance in multiple melanomas. Experimental Dermatology. https://doi.org/10.1111/exd.13951

Dermoscopic similarity is an independent predictor of BRAF mutational concordance in multiple melanomas. / Moscarella, Elvira; Pellegrini, Cristina; Pampena, Riccardo; Argenziano, Giuseppe; Manfredini, Marco; Martorelli, Claudia; Ciarrocchi, Alessia; Dika, Emi; Peris, Ketty; Antonini, Ambra; Cipolloni, Gianluca; Alfano, Roberto; Longo, Caterina; Fargnoli, Maria Concetta.

In: Experimental Dermatology, 01.01.2019.

Research output: Contribution to journal › Article

Moscarella, E, Pellegrini, C, Pampena, R, Argenziano, G, Manfredini, M, Martorelli, C, Ciarrocchi, A, Dika, E, Peris, K, Antonini, A, Cipolloni, G, Alfano, R, Longo, C & Fargnoli, MC 2019, 'Dermoscopic similarity is an independent predictor of BRAF mutational concordance in multiple melanomas', Experimental Dermatology. https://doi.org/10.1111/exd.13951
Moscarella E, Pellegrini C, Pampena R, Argenziano G, Manfredini M, Martorelli C et al. Dermoscopic similarity is an independent predictor of BRAF mutational concordance in multiple melanomas. Experimental Dermatology. 2019 Jan 1. https://doi.org/10.1111/exd.13951
Moscarella, Elvira ; Pellegrini, Cristina ; Pampena, Riccardo ; Argenziano, Giuseppe ; Manfredini, Marco ; Martorelli, Claudia ; Ciarrocchi, Alessia ; Dika, Emi ; Peris, Ketty ; Antonini, Ambra ; Cipolloni, Gianluca ; Alfano, Roberto ; Longo, Caterina ; Fargnoli, Maria Concetta. / Dermoscopic similarity is an independent predictor of BRAF mutational concordance in multiple melanomas. In: Experimental Dermatology. 2019.
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title = "Dermoscopic similarity is an independent predictor of BRAF mutational concordance in multiple melanomas",
abstract = "Background: The association of clinical and dermoscopic features with BRAF mutational status has been poorly analysed in multiple primary melanomas (MPM). Objective: To investigate whether concordance of BRAF mutational status is associated with dermoscopic similarity in multiple melanomas of the same patient. Methods: Dermoscopic images and corresponding tissue sections of 124 melanomas from 62 patients with MPM were selected at four Italian Dermatology Departments. Similarity of dermoscopic appearance between multiple melanomas was evaluated according to the presence of the same prevalent dermoscopic feature. The BRAF V600 mutational status was analysed with allele-specific TaqMan TM assays or pyrosequencing. Spearman's correlation and univariate and multivariate regression analysis were used for statistical analysis. Results: A similar dermoscopic appearance was identified in 38.7{\%} (24/62) of patients with MPM and was correlated with older age at first diagnosis (rho: 0.26; P: 0.042) and occurrence on sun-damaged skin (rho: 0.27; P: 0.037). The BRAF V600 mutation was detected in 39.5{\%} (49/124) of the tumors and a concordant BRAF mutational status between melanomas in 33/62 (53.2{\%}) MPM patients. Dermoscopically similar melanomas showed 5.7-fold higher odds to be concordant for BRAF mutational status compared to dissimilar lesions (OR: 5.7; 95{\%} CI 1.7-19.5; P: 0.005). Conclusion: Dermoscopic similarity of multiple melanomas represents an independent clinical predictor of a concordant BRAF mutational status in MPM patients.",
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author = "Elvira Moscarella and Cristina Pellegrini and Riccardo Pampena and Giuseppe Argenziano and Marco Manfredini and Claudia Martorelli and Alessia Ciarrocchi and Emi Dika and Ketty Peris and Ambra Antonini and Gianluca Cipolloni and Roberto Alfano and Caterina Longo and Fargnoli, {Maria Concetta}",
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AU - Moscarella, Elvira

AU - Pellegrini, Cristina

AU - Pampena, Riccardo

AU - Argenziano, Giuseppe

AU - Manfredini, Marco

AU - Martorelli, Claudia

AU - Ciarrocchi, Alessia

AU - Dika, Emi

AU - Peris, Ketty

AU - Antonini, Ambra

AU - Cipolloni, Gianluca

AU - Alfano, Roberto

AU - Longo, Caterina

AU - Fargnoli, Maria Concetta

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Background: The association of clinical and dermoscopic features with BRAF mutational status has been poorly analysed in multiple primary melanomas (MPM). Objective: To investigate whether concordance of BRAF mutational status is associated with dermoscopic similarity in multiple melanomas of the same patient. Methods: Dermoscopic images and corresponding tissue sections of 124 melanomas from 62 patients with MPM were selected at four Italian Dermatology Departments. Similarity of dermoscopic appearance between multiple melanomas was evaluated according to the presence of the same prevalent dermoscopic feature. The BRAF V600 mutational status was analysed with allele-specific TaqMan TM assays or pyrosequencing. Spearman's correlation and univariate and multivariate regression analysis were used for statistical analysis. Results: A similar dermoscopic appearance was identified in 38.7% (24/62) of patients with MPM and was correlated with older age at first diagnosis (rho: 0.26; P: 0.042) and occurrence on sun-damaged skin (rho: 0.27; P: 0.037). The BRAF V600 mutation was detected in 39.5% (49/124) of the tumors and a concordant BRAF mutational status between melanomas in 33/62 (53.2%) MPM patients. Dermoscopically similar melanomas showed 5.7-fold higher odds to be concordant for BRAF mutational status compared to dissimilar lesions (OR: 5.7; 95% CI 1.7-19.5; P: 0.005). Conclusion: Dermoscopic similarity of multiple melanomas represents an independent clinical predictor of a concordant BRAF mutational status in MPM patients.

AB - Background: The association of clinical and dermoscopic features with BRAF mutational status has been poorly analysed in multiple primary melanomas (MPM). Objective: To investigate whether concordance of BRAF mutational status is associated with dermoscopic similarity in multiple melanomas of the same patient. Methods: Dermoscopic images and corresponding tissue sections of 124 melanomas from 62 patients with MPM were selected at four Italian Dermatology Departments. Similarity of dermoscopic appearance between multiple melanomas was evaluated according to the presence of the same prevalent dermoscopic feature. The BRAF V600 mutational status was analysed with allele-specific TaqMan TM assays or pyrosequencing. Spearman's correlation and univariate and multivariate regression analysis were used for statistical analysis. Results: A similar dermoscopic appearance was identified in 38.7% (24/62) of patients with MPM and was correlated with older age at first diagnosis (rho: 0.26; P: 0.042) and occurrence on sun-damaged skin (rho: 0.27; P: 0.037). The BRAF V600 mutation was detected in 39.5% (49/124) of the tumors and a concordant BRAF mutational status between melanomas in 33/62 (53.2%) MPM patients. Dermoscopically similar melanomas showed 5.7-fold higher odds to be concordant for BRAF mutational status compared to dissimilar lesions (OR: 5.7; 95% CI 1.7-19.5; P: 0.005). Conclusion: Dermoscopic similarity of multiple melanomas represents an independent clinical predictor of a concordant BRAF mutational status in MPM patients.

KW - BRAF

KW - dermoscopy

KW - molecular analyses

KW - multiple primary melanomas

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DO - 10.1111/exd.13951

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