Description and molecular modeling of two novel HLA alleles: HLA-A*0343 and A*0345

Sara Frison, Pietro Crivello, Elena Longhi, Emanuela Andreini, Mara Tivelli, Marta Serafini, Cinzia Tagliaferri, Mario Scalamogna, Francesca Poli

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We report the identification of two novel human leucocyte antigen (HLA) in two Caucasian individuals. HLA-A*0343 differs from A03010101 by four changes at nucleotides 411-414 (CCGG→TGAA) and by a point mutation at position 418 (G→C). These differences lead to two amino acid substitutions at codon 114, where arginine has changed into negatively charged glutamic acid, and at codon 116, where aspartic acid has changed into positively charged histidine. Molecular modeling showed that these changes have a profound influence on the overall charge of the F pocket of the groove, resulting in potentially important changes in the peptide repertoire. HLA-A*0345 was found in a hematological female patient candidate to bone marrow transplantation. This new variant differs from HLA-A03010101 at position 845 (C→A) encoding an amino acid change of threonine to asparagine at codon 258 located in the α3 domain. Molecular modeling does not suggest a substantial role of this substitutions on the interaction with β2-microglobulin or CD8.

Original languageEnglish
Pages (from-to)582-585
Number of pages4
JournalHuman Immunology
Volume71
Issue number6
DOIs
Publication statusPublished - Jun 2010

Fingerprint

HLA Antigens
Alleles
Codon
Asparagine
Threonine
Amino Acid Substitution
Bone Marrow Transplantation
Point Mutation
Histidine
Aspartic Acid
Arginine
Glutamic Acid
Nucleotides
Amino Acids
Peptides

Keywords

  • HLA-A*0343
  • HLA-A*0345
  • SBT
  • Tissue typing

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Medicine(all)

Cite this

Description and molecular modeling of two novel HLA alleles : HLA-A*0343 and A*0345. / Frison, Sara; Crivello, Pietro; Longhi, Elena; Andreini, Emanuela; Tivelli, Mara; Serafini, Marta; Tagliaferri, Cinzia; Scalamogna, Mario; Poli, Francesca.

In: Human Immunology, Vol. 71, No. 6, 06.2010, p. 582-585.

Research output: Contribution to journalArticle

Frison, S, Crivello, P, Longhi, E, Andreini, E, Tivelli, M, Serafini, M, Tagliaferri, C, Scalamogna, M & Poli, F 2010, 'Description and molecular modeling of two novel HLA alleles: HLA-A*0343 and A*0345', Human Immunology, vol. 71, no. 6, pp. 582-585. https://doi.org/10.1016/j.humimm.2010.03.005
Frison, Sara ; Crivello, Pietro ; Longhi, Elena ; Andreini, Emanuela ; Tivelli, Mara ; Serafini, Marta ; Tagliaferri, Cinzia ; Scalamogna, Mario ; Poli, Francesca. / Description and molecular modeling of two novel HLA alleles : HLA-A*0343 and A*0345. In: Human Immunology. 2010 ; Vol. 71, No. 6. pp. 582-585.
@article{6643d492e2ab470f9af4d6bcf2784cdb,
title = "Description and molecular modeling of two novel HLA alleles: HLA-A*0343 and A*0345",
abstract = "We report the identification of two novel human leucocyte antigen (HLA) in two Caucasian individuals. HLA-A*0343 differs from A03010101 by four changes at nucleotides 411-414 (CCGG→TGAA) and by a point mutation at position 418 (G→C). These differences lead to two amino acid substitutions at codon 114, where arginine has changed into negatively charged glutamic acid, and at codon 116, where aspartic acid has changed into positively charged histidine. Molecular modeling showed that these changes have a profound influence on the overall charge of the F pocket of the groove, resulting in potentially important changes in the peptide repertoire. HLA-A*0345 was found in a hematological female patient candidate to bone marrow transplantation. This new variant differs from HLA-A03010101 at position 845 (C→A) encoding an amino acid change of threonine to asparagine at codon 258 located in the α3 domain. Molecular modeling does not suggest a substantial role of this substitutions on the interaction with β2-microglobulin or CD8.",
keywords = "HLA-A*0343, HLA-A*0345, SBT, Tissue typing",
author = "Sara Frison and Pietro Crivello and Elena Longhi and Emanuela Andreini and Mara Tivelli and Marta Serafini and Cinzia Tagliaferri and Mario Scalamogna and Francesca Poli",
year = "2010",
month = "6",
doi = "10.1016/j.humimm.2010.03.005",
language = "English",
volume = "71",
pages = "582--585",
journal = "Human Immunology",
issn = "0198-8859",
publisher = "Elsevier Inc.",
number = "6",

}

TY - JOUR

T1 - Description and molecular modeling of two novel HLA alleles

T2 - HLA-A*0343 and A*0345

AU - Frison, Sara

AU - Crivello, Pietro

AU - Longhi, Elena

AU - Andreini, Emanuela

AU - Tivelli, Mara

AU - Serafini, Marta

AU - Tagliaferri, Cinzia

AU - Scalamogna, Mario

AU - Poli, Francesca

PY - 2010/6

Y1 - 2010/6

N2 - We report the identification of two novel human leucocyte antigen (HLA) in two Caucasian individuals. HLA-A*0343 differs from A03010101 by four changes at nucleotides 411-414 (CCGG→TGAA) and by a point mutation at position 418 (G→C). These differences lead to two amino acid substitutions at codon 114, where arginine has changed into negatively charged glutamic acid, and at codon 116, where aspartic acid has changed into positively charged histidine. Molecular modeling showed that these changes have a profound influence on the overall charge of the F pocket of the groove, resulting in potentially important changes in the peptide repertoire. HLA-A*0345 was found in a hematological female patient candidate to bone marrow transplantation. This new variant differs from HLA-A03010101 at position 845 (C→A) encoding an amino acid change of threonine to asparagine at codon 258 located in the α3 domain. Molecular modeling does not suggest a substantial role of this substitutions on the interaction with β2-microglobulin or CD8.

AB - We report the identification of two novel human leucocyte antigen (HLA) in two Caucasian individuals. HLA-A*0343 differs from A03010101 by four changes at nucleotides 411-414 (CCGG→TGAA) and by a point mutation at position 418 (G→C). These differences lead to two amino acid substitutions at codon 114, where arginine has changed into negatively charged glutamic acid, and at codon 116, where aspartic acid has changed into positively charged histidine. Molecular modeling showed that these changes have a profound influence on the overall charge of the F pocket of the groove, resulting in potentially important changes in the peptide repertoire. HLA-A*0345 was found in a hematological female patient candidate to bone marrow transplantation. This new variant differs from HLA-A03010101 at position 845 (C→A) encoding an amino acid change of threonine to asparagine at codon 258 located in the α3 domain. Molecular modeling does not suggest a substantial role of this substitutions on the interaction with β2-microglobulin or CD8.

KW - HLA-A0343

KW - HLA-A0345

KW - SBT

KW - Tissue typing

UR - http://www.scopus.com/inward/record.url?scp=77952884956&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77952884956&partnerID=8YFLogxK

U2 - 10.1016/j.humimm.2010.03.005

DO - 10.1016/j.humimm.2010.03.005

M3 - Article

C2 - 20307618

AN - SCOPUS:77952884956

VL - 71

SP - 582

EP - 585

JO - Human Immunology

JF - Human Immunology

SN - 0198-8859

IS - 6

ER -