Design and characterization of a chitosan physical gel promoting wound healing in mice

Laura Mayol, Daniela De Stefano, Virginia Campani, Francesca De Falco, Eleonora Ferrari, Claudia Cencetti, Pietro Matricardi, Luigi Maiuri, Rosa Carnuccio, Angela Gallo, Maria Chiara Maiuri, Giuseppe De Rosa

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, a sterile and biocompatible chitosan (CHI) gel for wound healing applications was formulated. CHI powder was treated in autoclave (ttCHI) to prepare sterile formulations. The heat treatment modified the CHI molecular weight, as evidenced by GPC analysis, and its physical-chemical features. Differential scanning calorimetry studies indicated that the macromolecules, before and after thermal treatment, differ in the strength of water-polymer interaction leading to different viscoelastic and flow properties. Thermally treated CHI exhibited the following effects: (i) increased the proliferation and migration of human foreskin foetal fibroblasts at 24 h; (ii) accelerated wound healing (measured as area of lesion) at 3 and 10 days in an in vivo model of pressure ulcers. These effects were linked to the increase of the hydroxyproline and haemoglobin content as well as Wnt protein expression. Moreover, we found a reduction of myeloperoxidase activity and TNF-α mRNA expression. These observations suggest the potential of this novel CHI gel in wound healing and other therapeutic applications.

Original languageEnglish
Pages (from-to)1483-1493
Number of pages11
JournalJournal of Materials Science: Materials in Medicine
Volume25
Issue number6
DOIs
Publication statusPublished - 2014

ASJC Scopus subject areas

  • Biophysics
  • Biomaterials
  • Bioengineering
  • Biomedical Engineering

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