Design and construction of a naïve mouse antibody phage display library

R. Sommavilla, V. Lovato, A. Villa, D. Sgier, D. Neri

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Antibody phage technology has greatly facilitated the isolation of good-quality monoclonal antibodies to virtually any target antigen. Large combinatorial phage display libraries of human antibodies are routinely being used for the identification of antibody candidates for clinical applications. However, preclinical studies in rodents would benefit from the availability of good-quality single-pot mouse antibody libraries, which at present are not available. In this article, we report on the construction of three mouse antibody phage display libraries, all containing over 1 billion antibody clones and all based on a similar library design, which featured the combinatorial mutagenesis of residues in the CDR3 loops of a given antibody scaffold. While all three libraries were found to express antibodies in bacterial supernatants, only one of them (termed "PHILOtop") was shown to reliably yield good-quality antibodies towards all protein antigens used so far in selection experiments, including three tumor-associated antigens. The modular structure of the PHILOtop library facilitates a simple affinity-maturation procedure based on the combinatorial mutagenesis of CDR1 and CDR2 loops of the VH domain, which has led to the isolation of a high-affinity antibody ("H7"; Kd=6 nM) specific to the EDB domain of fibronectin, a marker of angiogenesis. The single-pot antibody library PHILOtop may thus represent a useful source of binding specificities, facilitating preclinical studies in immunocompetent syngeneic mouse models of pathology.

Original languageEnglish
Pages (from-to)31-43
Number of pages13
JournalJournal of Immunological Methods
Volume353
Issue number1-2
DOIs
Publication statusPublished - Feb 2010

Fingerprint

Bacteriophages
Libraries
Antibodies
Mutagenesis
Bacterial Antibodies
Antigens
Antibody Affinity
Neoplasm Antigens
Fibronectins
Rodentia
Clone Cells
Monoclonal Antibodies
Pathology
Technology

Keywords

  • Antibody library
  • Antibody phage display
  • Single chain Fv antibody fragment

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Design and construction of a naïve mouse antibody phage display library. / Sommavilla, R.; Lovato, V.; Villa, A.; Sgier, D.; Neri, D.

In: Journal of Immunological Methods, Vol. 353, No. 1-2, 02.2010, p. 31-43.

Research output: Contribution to journalArticle

Sommavilla, R, Lovato, V, Villa, A, Sgier, D & Neri, D 2010, 'Design and construction of a naïve mouse antibody phage display library', Journal of Immunological Methods, vol. 353, no. 1-2, pp. 31-43. https://doi.org/10.1016/j.jim.2010.01.003
Sommavilla, R. ; Lovato, V. ; Villa, A. ; Sgier, D. ; Neri, D. / Design and construction of a naïve mouse antibody phage display library. In: Journal of Immunological Methods. 2010 ; Vol. 353, No. 1-2. pp. 31-43.
@article{0d0ca2ed537b448da414abd4273236d7,
title = "Design and construction of a na{\"i}ve mouse antibody phage display library",
abstract = "Antibody phage technology has greatly facilitated the isolation of good-quality monoclonal antibodies to virtually any target antigen. Large combinatorial phage display libraries of human antibodies are routinely being used for the identification of antibody candidates for clinical applications. However, preclinical studies in rodents would benefit from the availability of good-quality single-pot mouse antibody libraries, which at present are not available. In this article, we report on the construction of three mouse antibody phage display libraries, all containing over 1 billion antibody clones and all based on a similar library design, which featured the combinatorial mutagenesis of residues in the CDR3 loops of a given antibody scaffold. While all three libraries were found to express antibodies in bacterial supernatants, only one of them (termed {"}PHILOtop{"}) was shown to reliably yield good-quality antibodies towards all protein antigens used so far in selection experiments, including three tumor-associated antigens. The modular structure of the PHILOtop library facilitates a simple affinity-maturation procedure based on the combinatorial mutagenesis of CDR1 and CDR2 loops of the VH domain, which has led to the isolation of a high-affinity antibody ({"}H7{"}; Kd=6 nM) specific to the EDB domain of fibronectin, a marker of angiogenesis. The single-pot antibody library PHILOtop may thus represent a useful source of binding specificities, facilitating preclinical studies in immunocompetent syngeneic mouse models of pathology.",
keywords = "Antibody library, Antibody phage display, Single chain Fv antibody fragment",
author = "R. Sommavilla and V. Lovato and A. Villa and D. Sgier and D. Neri",
year = "2010",
month = "2",
doi = "10.1016/j.jim.2010.01.003",
language = "English",
volume = "353",
pages = "31--43",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Design and construction of a naïve mouse antibody phage display library

AU - Sommavilla, R.

AU - Lovato, V.

AU - Villa, A.

AU - Sgier, D.

AU - Neri, D.

PY - 2010/2

Y1 - 2010/2

N2 - Antibody phage technology has greatly facilitated the isolation of good-quality monoclonal antibodies to virtually any target antigen. Large combinatorial phage display libraries of human antibodies are routinely being used for the identification of antibody candidates for clinical applications. However, preclinical studies in rodents would benefit from the availability of good-quality single-pot mouse antibody libraries, which at present are not available. In this article, we report on the construction of three mouse antibody phage display libraries, all containing over 1 billion antibody clones and all based on a similar library design, which featured the combinatorial mutagenesis of residues in the CDR3 loops of a given antibody scaffold. While all three libraries were found to express antibodies in bacterial supernatants, only one of them (termed "PHILOtop") was shown to reliably yield good-quality antibodies towards all protein antigens used so far in selection experiments, including three tumor-associated antigens. The modular structure of the PHILOtop library facilitates a simple affinity-maturation procedure based on the combinatorial mutagenesis of CDR1 and CDR2 loops of the VH domain, which has led to the isolation of a high-affinity antibody ("H7"; Kd=6 nM) specific to the EDB domain of fibronectin, a marker of angiogenesis. The single-pot antibody library PHILOtop may thus represent a useful source of binding specificities, facilitating preclinical studies in immunocompetent syngeneic mouse models of pathology.

AB - Antibody phage technology has greatly facilitated the isolation of good-quality monoclonal antibodies to virtually any target antigen. Large combinatorial phage display libraries of human antibodies are routinely being used for the identification of antibody candidates for clinical applications. However, preclinical studies in rodents would benefit from the availability of good-quality single-pot mouse antibody libraries, which at present are not available. In this article, we report on the construction of three mouse antibody phage display libraries, all containing over 1 billion antibody clones and all based on a similar library design, which featured the combinatorial mutagenesis of residues in the CDR3 loops of a given antibody scaffold. While all three libraries were found to express antibodies in bacterial supernatants, only one of them (termed "PHILOtop") was shown to reliably yield good-quality antibodies towards all protein antigens used so far in selection experiments, including three tumor-associated antigens. The modular structure of the PHILOtop library facilitates a simple affinity-maturation procedure based on the combinatorial mutagenesis of CDR1 and CDR2 loops of the VH domain, which has led to the isolation of a high-affinity antibody ("H7"; Kd=6 nM) specific to the EDB domain of fibronectin, a marker of angiogenesis. The single-pot antibody library PHILOtop may thus represent a useful source of binding specificities, facilitating preclinical studies in immunocompetent syngeneic mouse models of pathology.

KW - Antibody library

KW - Antibody phage display

KW - Single chain Fv antibody fragment

UR - http://www.scopus.com/inward/record.url?scp=77649270850&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77649270850&partnerID=8YFLogxK

U2 - 10.1016/j.jim.2010.01.003

DO - 10.1016/j.jim.2010.01.003

M3 - Article

C2 - 20093119

AN - SCOPUS:77649270850

VL - 353

SP - 31

EP - 43

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

IS - 1-2

ER -