Desmin-positive pericytes in the chick embryo chorioallantoic membrane in response to fibroblast growth factor-2

Beatrice Nico, Maria Grazia Ennas, Enrico Crivellato, Annalisa Frontino, Domenica Mangieri, Michela De Giorgis, Luisa Roncali, Domenico Ribatti

Research output: Contribution to journalArticle

Abstract

Pericytes stabilize vessel wall, participate in the regulation of blood flow microcirculation and influence endothelial proliferation, survival, migration and maturation. Pericytes are commonly identified by molecular markers, such as desmin. The aim of this study was to analyze the pattern of distribution of desmin in the chick embryo chorioallantoic membrane (CAM) vasculature in normal condition and in response to an angiogenic cytokine, such as fibroblast growth factor-2 (FGF-2) by using conventional immunohistochemistry and confocal laser scanning microscopy. Results indicate that, in normal condition, the desmin-positive pericytes are located around the endothelial cells of the capillary plexus and of larger vessels in the intermediate mesenchyme. In response to FGF-2 numerous desmin-positive and α smooth muscle actin-negative cells are located in the mesenchyme and numerous blood vessels positive to factor VIII and desmin are recognizable near to the gelatin sponge soaked with FGF-2. These data suggest that pericytes in normal condition intervene to stabilize the wall of newly formed blood vessel, while in response to FGF-2, they detach from the wall of parental vessels and migrate, as extramural fibroblast-like desmin-positive cells, in the surrounding mesenchyme, where they modulate the angiogenic response.

Original languageEnglish
Pages (from-to)13-19
Number of pages7
JournalMicrovascular Research
Volume68
Issue number1
DOIs
Publication statusPublished - Jul 2004

Fingerprint

Chorioallantoic Membrane
Pericytes
Desmin
Fibroblast Growth Factor 2
Chick Embryo
Membranes
Mesoderm
Blood vessels
Blood Vessels
Microcirculation
Endothelial cells
Factor VIII
Porifera
Fibroblasts
Gelatin
Confocal Microscopy
Smooth Muscle
Muscle
Actins
Microscopic examination

Keywords

  • Angiogenesis
  • Chorioallantoic membrane
  • Desmin
  • Fibroblast growth factor-2
  • Pericyte

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine

Cite this

Nico, B., Ennas, M. G., Crivellato, E., Frontino, A., Mangieri, D., De Giorgis, M., ... Ribatti, D. (2004). Desmin-positive pericytes in the chick embryo chorioallantoic membrane in response to fibroblast growth factor-2. Microvascular Research, 68(1), 13-19. https://doi.org/10.1016/j.mvr.2004.03.005

Desmin-positive pericytes in the chick embryo chorioallantoic membrane in response to fibroblast growth factor-2. / Nico, Beatrice; Ennas, Maria Grazia; Crivellato, Enrico; Frontino, Annalisa; Mangieri, Domenica; De Giorgis, Michela; Roncali, Luisa; Ribatti, Domenico.

In: Microvascular Research, Vol. 68, No. 1, 07.2004, p. 13-19.

Research output: Contribution to journalArticle

Nico, B, Ennas, MG, Crivellato, E, Frontino, A, Mangieri, D, De Giorgis, M, Roncali, L & Ribatti, D 2004, 'Desmin-positive pericytes in the chick embryo chorioallantoic membrane in response to fibroblast growth factor-2', Microvascular Research, vol. 68, no. 1, pp. 13-19. https://doi.org/10.1016/j.mvr.2004.03.005
Nico, Beatrice ; Ennas, Maria Grazia ; Crivellato, Enrico ; Frontino, Annalisa ; Mangieri, Domenica ; De Giorgis, Michela ; Roncali, Luisa ; Ribatti, Domenico. / Desmin-positive pericytes in the chick embryo chorioallantoic membrane in response to fibroblast growth factor-2. In: Microvascular Research. 2004 ; Vol. 68, No. 1. pp. 13-19.
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