Detailed mapping and loss of heterozygosity analysis suggests a suppressor locus involved in sporadic breast cancer within a distal region of chromosome band 17p13.3

M. Stack, D. Jones, G. White, D. S. Liscia, T. Venesio, G. Casey, D. Crichton, J. Varley, E. Mitchell, J. Heighway, M. Santibanez-Koref

Research output: Contribution to journalArticlepeer-review

Abstract

The chromosome region 17p13.3 is thought to encode a tumour suppressor gene involved in sporadic breast cancer and other malignancies. Physical ordering of markers has been carried out by a series of multicolour fluorescent in situ hybridisation (FISH) experiments, using isolated yeast artificial chromosomes (YACs) and cosmids. Eight polymorphic markers ordered within this new physical map and one external marker were used to investigate the pattern of loss of heterozygosity in a panel of 40 sporadic breast tumour patients. The data revealed a region of high loss (60%) within distal 17p13.3, defined by markers D17S926, D17S695 and D17S849 which mapped close together. A contig of YACs was constructed physically linking these three markers.

Original languageEnglish
Pages (from-to)2047-2055
Number of pages9
JournalHuman Molecular Genetics
Volume4
Issue number11
Publication statusPublished - 1995

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Genetics(clinical)
  • Public Health, Environmental and Occupational Health

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