Detecting deep venous thrombosis with technetium-99m-labeled synthetic peptide P280

P. Muto, S. Lastoria, P. Varrella, E. Vergara, M. Salvatore, G. Morgano, J. Lister- James, J. D. Bernardy, R. T. Dean, D. Wencker, J. S. Borer

Research output: Contribution to journalArticle

Abstract

Scintigraphy, using small, thrombus-avid, synthetic peptides labeled with gamma-emitting nuclides is an innovative approach to the noninvasive detection of acute deep venous thrombosis (DVT). The goal of this study was to evaluate clinically 99mTc-P280 for imaging DVT. The peptide P280 is a 26 amino acid dimer that binds with high affinity to the GPIIb/IIIa receptor expressed on activated platelets and can be labeled with 99mTc. Methods: Scintigraphy with 99mTc-P280 (10-22 mCi) was performed in nine patients with clinical suspicion and diagnostic evidence of DVT. Planar and tomographic images of the legs, abdomen/pelvis, chest and head were obtained immediately, 1, 2, 4 and 24 hr after injection. Results: No adverse effects were noted after 99mTc-P280 administration in any patient. Positive visualization of thrombi occurred in eight of nine cases with confirmed DVT within 1 hr of tracer injection. The majority of the patients had recent onset of DVT symptoms (less than 3 wk), while the only negative case was diagnosed 42 days earlier and was likely related to an accident 7 mo earlier. Thrombi-to-background ratios were essentially constant over the study. Technetium-99m-P280 accumulation was also discernible in two patients with pulmonary embolism, while in a third patient the radiotracer concentrated in a cerebellar hemangioblastoma. Conclusion: These human studies indicate that 99mTc-P280 is a potentially safe and sensitive procedure for diagnosing DVT and pulmonary embolism. It also may have substantial utility in monitoring active venous thrombosis.

Original languageEnglish
Pages (from-to)1384-1391
Number of pages8
JournalJournal of Nuclear Medicine
Volume36
Issue number8
Publication statusPublished - 1995

Keywords

  • deep venous thrombosis
  • receptor imaging
  • technetium-99m-labeled peptides

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology

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    Muto, P., Lastoria, S., Varrella, P., Vergara, E., Salvatore, M., Morgano, G., Lister- James, J., Bernardy, J. D., Dean, R. T., Wencker, D., & Borer, J. S. (1995). Detecting deep venous thrombosis with technetium-99m-labeled synthetic peptide P280. Journal of Nuclear Medicine, 36(8), 1384-1391.