Detecting immunoglobulin abnormalities in the cerebrospinal fluid (CSF): Which methods are best for clinical purposes?

M. A. Laurenzi, L. Provinciali, A. R. Giovagnoli, [No Value] Busco, V. Bocchini, L. Paris, F. Chiodi

Research output: Contribution to journalArticlepeer-review

Abstract

Several techniques are now available for the quantitative and qualitative examination of CSF proteins modified during the course of neurological disease. The CSF and serum of 42 patients suffering from various neurological diseases and of 7 controls were examined using quantitative methods-single radial immunodiffusion, nephelometric analysis-and qualitative methods-(i) isoelectric-focusing on polyacrylamide gel and (ii) on agarose gel, isoelectric focusing of unconcentrated CSF followed by transfer to cellulose nitrate membranes and (iii) immunoperoxidase staining and (iv) double antibody peroxidase labeling and avidin-biotin amplification. In the present study the results and the advantages of different methods are described and compared.

Original languageEnglish
Pages (from-to)237-243
Number of pages7
JournalItalian Journal of Neurological Sciences
Volume8
Issue number3
DOIs
Publication statusPublished - Jun 1987

Keywords

  • avidin-biotin amplification
  • CSF immunoglobulins
  • nephelometric analysis-isoelectric focusing on polyaerylamide and agarose gelimmunoperoxidase staining
  • quantitative and qualitative methods
  • single radial immunodiffusion

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology

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