Detection and characterization of different brain-derived subpopulations of plasma exosomes by Surface Plasmon Resonance imaging

Silvia Picciolini, Alice Gualerzi, Renzo Vanna, Andrea Sguassero, Furio Gramatica, Marzia Bedoni, Massimo Masserini, Carlo Francesco Morasso

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The use of exosomes for diagnostic and disease monitoring purposes is becoming particularly appealing in biomedical research because of the possibility to study directly in biological fluids some of the features related to the organs from which exosomes originate. A paradigmatic example are brain-derived exosomes that can be found in plasma and used as a direct read-out of the status of the central nervous system (CNS). Inspired by recent remarkable development of plasmonic biosensors, we have designed a Surface Plasmon Resonance imaging (SPRi) assay that, taking advantage from the fact that exosomes size perfectly fits within the surface plasmon wave depth, allows the detection of multiple exosome subpopulations of neural origin directly in blood. By using an array of antibodies, exosomes derived from neurons and oligodendrocytes were isolated and detected with good sensitivity. Subsequently, by injecting a second antibody on the immobilized vesicles, we were able to quantify the amount of CD81 and GM1, membrane components of exosomes, on each subpopulation. In this way we have been able to demonstrate that they are not homogeneously expressed but exhibit a variable abundance according to the exosomes cellular origin. These results confirm the extreme variability of exosomes composition and how SPRi can provide an effective tool for their characterization. Besides, our work paves the road towards more precise clinical studies on the use of exosomes as potential biomarkers of neurodegenerative diseases.

Original languageEnglish
JournalAnalytical Chemistry
DOIs
Publication statusAccepted/In press - Feb 28 2018

Fingerprint

Surface plasmon resonance
Brain
Neurodegenerative diseases
Plasmas
Imaging techniques
Antibodies
Neurology
Biomarkers
Biosensors
Neurons
Assays
Blood
Membranes
Fluids
Monitoring
Chemical analysis

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Detection and characterization of different brain-derived subpopulations of plasma exosomes by Surface Plasmon Resonance imaging. / Picciolini, Silvia; Gualerzi, Alice; Vanna, Renzo; Sguassero, Andrea; Gramatica, Furio; Bedoni, Marzia; Masserini, Massimo; Morasso, Carlo Francesco.

In: Analytical Chemistry, 28.02.2018.

Research output: Contribution to journalArticle

@article{09d7c6007f6b4590912fb24fe7a607f6,
title = "Detection and characterization of different brain-derived subpopulations of plasma exosomes by Surface Plasmon Resonance imaging",
abstract = "The use of exosomes for diagnostic and disease monitoring purposes is becoming particularly appealing in biomedical research because of the possibility to study directly in biological fluids some of the features related to the organs from which exosomes originate. A paradigmatic example are brain-derived exosomes that can be found in plasma and used as a direct read-out of the status of the central nervous system (CNS). Inspired by recent remarkable development of plasmonic biosensors, we have designed a Surface Plasmon Resonance imaging (SPRi) assay that, taking advantage from the fact that exosomes size perfectly fits within the surface plasmon wave depth, allows the detection of multiple exosome subpopulations of neural origin directly in blood. By using an array of antibodies, exosomes derived from neurons and oligodendrocytes were isolated and detected with good sensitivity. Subsequently, by injecting a second antibody on the immobilized vesicles, we were able to quantify the amount of CD81 and GM1, membrane components of exosomes, on each subpopulation. In this way we have been able to demonstrate that they are not homogeneously expressed but exhibit a variable abundance according to the exosomes cellular origin. These results confirm the extreme variability of exosomes composition and how SPRi can provide an effective tool for their characterization. Besides, our work paves the road towards more precise clinical studies on the use of exosomes as potential biomarkers of neurodegenerative diseases.",
author = "Silvia Picciolini and Alice Gualerzi and Renzo Vanna and Andrea Sguassero and Furio Gramatica and Marzia Bedoni and Massimo Masserini and Morasso, {Carlo Francesco}",
year = "2018",
month = "2",
day = "28",
doi = "10.1021/acs.analchem.8b00941",
language = "English",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",

}

TY - JOUR

T1 - Detection and characterization of different brain-derived subpopulations of plasma exosomes by Surface Plasmon Resonance imaging

AU - Picciolini, Silvia

AU - Gualerzi, Alice

AU - Vanna, Renzo

AU - Sguassero, Andrea

AU - Gramatica, Furio

AU - Bedoni, Marzia

AU - Masserini, Massimo

AU - Morasso, Carlo Francesco

PY - 2018/2/28

Y1 - 2018/2/28

N2 - The use of exosomes for diagnostic and disease monitoring purposes is becoming particularly appealing in biomedical research because of the possibility to study directly in biological fluids some of the features related to the organs from which exosomes originate. A paradigmatic example are brain-derived exosomes that can be found in plasma and used as a direct read-out of the status of the central nervous system (CNS). Inspired by recent remarkable development of plasmonic biosensors, we have designed a Surface Plasmon Resonance imaging (SPRi) assay that, taking advantage from the fact that exosomes size perfectly fits within the surface plasmon wave depth, allows the detection of multiple exosome subpopulations of neural origin directly in blood. By using an array of antibodies, exosomes derived from neurons and oligodendrocytes were isolated and detected with good sensitivity. Subsequently, by injecting a second antibody on the immobilized vesicles, we were able to quantify the amount of CD81 and GM1, membrane components of exosomes, on each subpopulation. In this way we have been able to demonstrate that they are not homogeneously expressed but exhibit a variable abundance according to the exosomes cellular origin. These results confirm the extreme variability of exosomes composition and how SPRi can provide an effective tool for their characterization. Besides, our work paves the road towards more precise clinical studies on the use of exosomes as potential biomarkers of neurodegenerative diseases.

AB - The use of exosomes for diagnostic and disease monitoring purposes is becoming particularly appealing in biomedical research because of the possibility to study directly in biological fluids some of the features related to the organs from which exosomes originate. A paradigmatic example are brain-derived exosomes that can be found in plasma and used as a direct read-out of the status of the central nervous system (CNS). Inspired by recent remarkable development of plasmonic biosensors, we have designed a Surface Plasmon Resonance imaging (SPRi) assay that, taking advantage from the fact that exosomes size perfectly fits within the surface plasmon wave depth, allows the detection of multiple exosome subpopulations of neural origin directly in blood. By using an array of antibodies, exosomes derived from neurons and oligodendrocytes were isolated and detected with good sensitivity. Subsequently, by injecting a second antibody on the immobilized vesicles, we were able to quantify the amount of CD81 and GM1, membrane components of exosomes, on each subpopulation. In this way we have been able to demonstrate that they are not homogeneously expressed but exhibit a variable abundance according to the exosomes cellular origin. These results confirm the extreme variability of exosomes composition and how SPRi can provide an effective tool for their characterization. Besides, our work paves the road towards more precise clinical studies on the use of exosomes as potential biomarkers of neurodegenerative diseases.

UR - http://www.scopus.com/inward/record.url?scp=85049657169&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85049657169&partnerID=8YFLogxK

U2 - 10.1021/acs.analchem.8b00941

DO - 10.1021/acs.analchem.8b00941

M3 - Article

AN - SCOPUS:85049657169

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

ER -