Detection and isolation of Shiga Toxin-producing Escherichia coli (STEC) strains in caecal samples from pigs at slaughter in Italy

Silvia Arancia, Manuela Iurescia, Serena Lorenzetti, Fiorentino Stravino, Carmela Buccella, Andrea Caprioli, Alessia Franco, Antonio Battisti, Stefano Morabito, Rosangela Tozzoli

Research output: Contribution to journalArticlepeer-review

Abstract

Shiga toxin-producing Escherichia coli (STEC) strains are food-borne pathogens of public health concern. Despite ruminants are the most important reservoir, STEC human infections have also been attributed to pigs. We examined for the presence of STEC in 234 samples of swine caecal content collected during the year 2015 at Italian abattoirs in the framework of the harmonized monitoring of antimicrobial resistance (Decision 2013/652/EU). The presence of stx genes was detected in 122 (52.1%) samples, which were subsequently subjected to STEC isolation and characterization. The analysis of the 66 isolated STEC strains showed that the majority of the isolates (74.2%) possessed the stx2a gene subtype, in a few cases (16.7%) in combination with stx2b or stx2c. Only 25.8% of isolates possessed the stx2e subtype, typical of swine-adapted STEC. None of the isolates possessed the intimin-coding eae gene and the majority of them did not belong to serogroups commonly associated with human infections. The results of this study suggest that pigs can be considered as potential reservoir of certain STEC types.

Original languageEnglish
Pages (from-to)462-469
Number of pages8
JournalVeterinary Medicine and Science
Volume5
Issue number3
DOIs
Publication statusPublished - Aug 2019

Keywords

  • Abattoirs
  • Animals
  • Cecum/microbiology
  • Disease Reservoirs/microbiology
  • Escherichia coli Infections/epidemiology
  • Humans
  • Italy/epidemiology
  • Prevalence
  • Serogroup
  • Shiga Toxin 2/genetics
  • Shiga-Toxigenic Escherichia coli/genetics
  • Swine
  • Swine Diseases/epidemiology
  • Virulence/genetics

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