TY - JOUR
T1 - Detection and monitoring of trisomy 8 by fluorescence in situ hybridization in acute myeloid leukemia
T2 - A multicentric study
AU - Cuneo, Antonio
AU - Bigoni, Renato
AU - Roberti, Maria Grazia
AU - Bardi, Antonella
AU - Rigolin, Gian Matteo
AU - Piva, Nadia
AU - Mancini, Marco
AU - Nanni, Mauro
AU - Alimena, Giuliana
AU - Mecucci, Cristina
AU - Matteucci, Caterina
AU - La Starza, Roberta
AU - Bernasconi, Paolo
AU - Cavigliano, Paola
AU - Genini, Emilia
AU - Zaccaria, Alfonso
AU - Testoni, Nicoletta
AU - Carboni, Cristina
AU - Castoldi, Gianluigi
PY - 1998/1
Y1 - 1998/1
N2 - Background and Objective. The role of fluorescence in situ hybridization (FISH) in the detection and monitoring of trisomy 8 (+8) in acute myelogenous leukemia (AML) has not been defined exactly. This multicentric study was performed in order to: I) analyze the sensitivity of interphase FISH with respect to conventional chromosome analysis (CCA) in detecting +8; II) compare the results of FISH and CCA in the quantitation of the frequency of +8-positive cells; III) analyze the possible role of FISH in the cytogenetic follow-up of patients with +8. Design and Methods. One hundred and ninety- eight nonconsecutive patients with a diagnosis of AML seen at five centers over a 3-year period were studied by CCA and FISH with a chromosome 8- specific centromeric probe. Two hundred interphase cells were scored in each test and the cutoff for the recognition of +8 was set at 3%. An irrelevant pericentromeric probe was used as negative control in those cases with an apparently normal karyotype and trisomy 8 in interphase cells. FISH studies were conducted at diagnosis and, in 14 cases with +8, on 1-5 occasions during follow-up. Results. Karyotype aberrations were seen in 121 cases (61.1%), with +8 being present in 38 of them (16 as the sole aberration). Interphase FISH detected +8 in 37/38 cases; in a patient with 1/10 metaphases with +8, 2.3% interphase cells with 3 signals were seen. Fourteen additional cases with occult +8 were detected by FISH, which showed 4-22% interphase cells with three signals; 6 patients had an abnormal karyotype without +8, 3 had a normal karyotype, 5 had no analyzable mitoses. In 24 cases with >15 analyzable metaphases, percent variations between CCA and FISH in the estimation of the size of the trisomic clone ranged between 0.4% and 51%, median value 22%. Underestimation of the percent of trisomy 8 by FISH occurred in all 10 cases with >90% +8 metaphases. In 7/14 cases investigated sequentially, FISH detected 535% trisomic cells in the BM after induction therapy (4 CR, 3 PR); 4 cases relapsed with +8 at 8-15 months. The absence of +8 in remission marrows was documented in the remaining 7 cases, 4 of which relapsed at 20-32 months. Interpretation and Conclusions. It is concluded that FISH was a valuable method in this multicentric study since it showed greater sensitivity than CCA in detecting minor clones with +8, in patients with both normal and abnormal karyotypes. The role of FISH in the cytogenetic follow-up of trisomies in AML patient, may be promising.
AB - Background and Objective. The role of fluorescence in situ hybridization (FISH) in the detection and monitoring of trisomy 8 (+8) in acute myelogenous leukemia (AML) has not been defined exactly. This multicentric study was performed in order to: I) analyze the sensitivity of interphase FISH with respect to conventional chromosome analysis (CCA) in detecting +8; II) compare the results of FISH and CCA in the quantitation of the frequency of +8-positive cells; III) analyze the possible role of FISH in the cytogenetic follow-up of patients with +8. Design and Methods. One hundred and ninety- eight nonconsecutive patients with a diagnosis of AML seen at five centers over a 3-year period were studied by CCA and FISH with a chromosome 8- specific centromeric probe. Two hundred interphase cells were scored in each test and the cutoff for the recognition of +8 was set at 3%. An irrelevant pericentromeric probe was used as negative control in those cases with an apparently normal karyotype and trisomy 8 in interphase cells. FISH studies were conducted at diagnosis and, in 14 cases with +8, on 1-5 occasions during follow-up. Results. Karyotype aberrations were seen in 121 cases (61.1%), with +8 being present in 38 of them (16 as the sole aberration). Interphase FISH detected +8 in 37/38 cases; in a patient with 1/10 metaphases with +8, 2.3% interphase cells with 3 signals were seen. Fourteen additional cases with occult +8 were detected by FISH, which showed 4-22% interphase cells with three signals; 6 patients had an abnormal karyotype without +8, 3 had a normal karyotype, 5 had no analyzable mitoses. In 24 cases with >15 analyzable metaphases, percent variations between CCA and FISH in the estimation of the size of the trisomic clone ranged between 0.4% and 51%, median value 22%. Underestimation of the percent of trisomy 8 by FISH occurred in all 10 cases with >90% +8 metaphases. In 7/14 cases investigated sequentially, FISH detected 535% trisomic cells in the BM after induction therapy (4 CR, 3 PR); 4 cases relapsed with +8 at 8-15 months. The absence of +8 in remission marrows was documented in the remaining 7 cases, 4 of which relapsed at 20-32 months. Interpretation and Conclusions. It is concluded that FISH was a valuable method in this multicentric study since it showed greater sensitivity than CCA in detecting minor clones with +8, in patients with both normal and abnormal karyotypes. The role of FISH in the cytogenetic follow-up of trisomies in AML patient, may be promising.
KW - Acute myeloid leukemia
KW - FISH
KW - Trisomy 8
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M3 - Article
C2 - 9542319
AN - SCOPUS:0031950812
VL - 83
SP - 21
EP - 26
JO - Haematologica
JF - Haematologica
SN - 0390-6078
IS - 1
ER -