Detection and Quantitation of Genetically Modified Maize (Bt-176 Transgenic Maize) by Applying Ligation Detection Reaction and Universal Array Technology

Roberta Bordoni, Alessandra Mezzelani, Clarissa Consolandi, Andrea Frosini, Ermanno Rizzi, Bianca Castiglioni, Claudia Salati, Nelson Marmiroli, Rosangela Marchelli, Luigi Rossi Bernardi, Cristina Battaglia, Gianluca De Bellis

Research output: Contribution to journalArticlepeer-review

Abstract

We have applied the ligation detection reaction (LDR) combined with a universal array approach to the detection and quantitation of the polymerase chain reaction (PCR) amplified cry1A(b) gene from Bt-176 transgenic maize. We demonstrated excellent specificity and high sensitivity. Down to 0.5 fmol (nearly 60 pg) of PCR amplified transgenic material was inequivocally detected with excellent linearity within the 0.1-2.0% range with respect to wild-type maize. We suggest the feasibility of extending the LDR/universal array format to detect in parallel several transgenic sequences that are being developed for food applications.

Original languageEnglish
Pages (from-to)1049-1054
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume52
Issue number5
Publication statusPublished - Mar 10 2004

Keywords

  • Bt-176 transgenic maize
  • Genetically modified organisms
  • GMOs
  • LDR
  • Ligation detection reaction
  • PCR
  • Polymerase chain reaction
  • Universal array

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

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