Detection and Quantitation of Genetically Modified Maize (Bt-176 Transgenic Maize) by Applying Ligation Detection Reaction and Universal Array Technology

Roberta Bordoni; Alessandra Mezzelani; Clarissa Consolandi; Andrea Frosini; Ermanno Rizzi; Bianca Castiglioni; Claudia Salati; Nelson Marmiroli; Rosangela Marchelli; Luigi Rossi Bernardi; Cristina Battaglia; Gianluca De Bellis

Detection and Quantitation of Genetically Modified Maize (Bt-176 Transgenic Maize) by Applying Ligation Detection Reaction and Universal Array Technology

Roberta Bordoni, Alessandra Mezzelani, Clarissa Consolandi, Andrea Frosini, Ermanno Rizzi, Bianca Castiglioni, Claudia Salati, Nelson Marmiroli, Rosangela Marchelli, Luigi Rossi Bernardi, Cristina Battaglia, Gianluca De Bellis

IRCCS Multimedica

Research output: Contribution to journal › Article › peer-review

Abstract

We have applied the ligation detection reaction (LDR) combined with a universal array approach to the detection and quantitation of the polymerase chain reaction (PCR) amplified cry1A(b) gene from Bt-176 transgenic maize. We demonstrated excellent specificity and high sensitivity. Down to 0.5 fmol (nearly 60 pg) of PCR amplified transgenic material was inequivocally detected with excellent linearity within the 0.1-2.0% range with respect to wild-type maize. We suggest the feasibility of extending the LDR/universal array format to detect in parallel several transgenic sequences that are being developed for food applications.

Original language	English
Pages (from-to)	1049-1054
Number of pages	6
Journal	Journal of Agricultural and Food Chemistry
Volume	52
Issue number	5
Publication status	Published - Mar 10 2004

Keywords

Bt-176 transgenic maize
Genetically modified organisms
GMOs
LDR
Ligation detection reaction
PCR
Polymerase chain reaction
Universal array

ASJC Scopus subject areas

Agricultural and Biological Sciences (miscellaneous)
Food Science
Chemistry (miscellaneous)

Cite this

Bordoni, R., Mezzelani, A., Consolandi, C., Frosini, A., Rizzi, E., Castiglioni, B., Salati, C., Marmiroli, N., Marchelli, R., Bernardi, L. R., Battaglia, C., & De Bellis, G. (2004). Detection and Quantitation of Genetically Modified Maize (Bt-176 Transgenic Maize) by Applying Ligation Detection Reaction and Universal Array Technology. Journal of Agricultural and Food Chemistry, 52(5), 1049-1054.

Detection and Quantitation of Genetically Modified Maize (Bt-176 Transgenic Maize) by Applying Ligation Detection Reaction and Universal Array Technology. / Bordoni, Roberta; Mezzelani, Alessandra; Consolandi, Clarissa et al.

In: Journal of Agricultural and Food Chemistry, Vol. 52, No. 5, 10.03.2004, p. 1049-1054.

Research output: Contribution to journal › Article › peer-review

Bordoni, R, Mezzelani, A, Consolandi, C, Frosini, A, Rizzi, E, Castiglioni, B, Salati, C, Marmiroli, N, Marchelli, R, Bernardi, LR, Battaglia, C & De Bellis, G 2004, 'Detection and Quantitation of Genetically Modified Maize (Bt-176 Transgenic Maize) by Applying Ligation Detection Reaction and Universal Array Technology', Journal of Agricultural and Food Chemistry, vol. 52, no. 5, pp. 1049-1054.

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abstract = "We have applied the ligation detection reaction (LDR) combined with a universal array approach to the detection and quantitation of the polymerase chain reaction (PCR) amplified cry1A(b) gene from Bt-176 transgenic maize. We demonstrated excellent specificity and high sensitivity. Down to 0.5 fmol (nearly 60 pg) of PCR amplified transgenic material was inequivocally detected with excellent linearity within the 0.1-2.0% range with respect to wild-type maize. We suggest the feasibility of extending the LDR/universal array format to detect in parallel several transgenic sequences that are being developed for food applications.",

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AU - Consolandi, Clarissa

AU - Frosini, Andrea

AU - Rizzi, Ermanno

AU - Castiglioni, Bianca

AU - Salati, Claudia

AU - Marmiroli, Nelson

AU - Marchelli, Rosangela

AU - Bernardi, Luigi Rossi

AU - Battaglia, Cristina

AU - De Bellis, Gianluca

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Detection and Quantitation of Genetically Modified Maize (Bt-176 Transgenic Maize) by Applying Ligation Detection Reaction and Universal Array Technology

Abstract

Keywords

ASJC Scopus subject areas

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