Abstract
We have applied the ligation detection reaction (LDR) combined with a universal array approach to the detection and quantitation of the polymerase chain reaction (PCR) amplified cry1A(b) gene from Bt-176 transgenic maize. We demonstrated excellent specificity and high sensitivity. Down to 0.5 fmol (nearly 60 pg) of PCR amplified transgenic material was inequivocally detected with excellent linearity within the 0.1-2.0% range with respect to wild-type maize. We suggest the feasibility of extending the LDR/universal array format to detect in parallel several transgenic sequences that are being developed for food applications.
| Original language | English |
|---|---|
| Pages (from-to) | 1049-1054 |
| Number of pages | 6 |
| Journal | Journal of Agricultural and Food Chemistry |
| Volume | 52 |
| Issue number | 5 |
| Publication status | Published - Mar 10 2004 |
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Keywords
- Bt-176 transgenic maize
- Genetically modified organisms
- GMOs
- LDR
- Ligation detection reaction
- PCR
- Polymerase chain reaction
- Universal array
ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Food Science
- Chemistry (miscellaneous)
Cite this
Detection and Quantitation of Genetically Modified Maize (Bt-176 Transgenic Maize) by Applying Ligation Detection Reaction and Universal Array Technology. / Bordoni, Roberta; Mezzelani, Alessandra; Consolandi, Clarissa; Frosini, Andrea; Rizzi, Ermanno; Castiglioni, Bianca; Salati, Claudia; Marmiroli, Nelson; Marchelli, Rosangela; Bernardi, Luigi Rossi; Battaglia, Cristina; De Bellis, Gianluca.
In: Journal of Agricultural and Food Chemistry, Vol. 52, No. 5, 10.03.2004, p. 1049-1054.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection and Quantitation of Genetically Modified Maize (Bt-176 Transgenic Maize) by Applying Ligation Detection Reaction and Universal Array Technology
AU - Bordoni, Roberta
AU - Mezzelani, Alessandra
AU - Consolandi, Clarissa
AU - Frosini, Andrea
AU - Rizzi, Ermanno
AU - Castiglioni, Bianca
AU - Salati, Claudia
AU - Marmiroli, Nelson
AU - Marchelli, Rosangela
AU - Bernardi, Luigi Rossi
AU - Battaglia, Cristina
AU - De Bellis, Gianluca
PY - 2004/3/10
Y1 - 2004/3/10
N2 - We have applied the ligation detection reaction (LDR) combined with a universal array approach to the detection and quantitation of the polymerase chain reaction (PCR) amplified cry1A(b) gene from Bt-176 transgenic maize. We demonstrated excellent specificity and high sensitivity. Down to 0.5 fmol (nearly 60 pg) of PCR amplified transgenic material was inequivocally detected with excellent linearity within the 0.1-2.0% range with respect to wild-type maize. We suggest the feasibility of extending the LDR/universal array format to detect in parallel several transgenic sequences that are being developed for food applications.
AB - We have applied the ligation detection reaction (LDR) combined with a universal array approach to the detection and quantitation of the polymerase chain reaction (PCR) amplified cry1A(b) gene from Bt-176 transgenic maize. We demonstrated excellent specificity and high sensitivity. Down to 0.5 fmol (nearly 60 pg) of PCR amplified transgenic material was inequivocally detected with excellent linearity within the 0.1-2.0% range with respect to wild-type maize. We suggest the feasibility of extending the LDR/universal array format to detect in parallel several transgenic sequences that are being developed for food applications.
KW - Bt-176 transgenic maize
KW - Genetically modified organisms
KW - GMOs
KW - LDR
KW - Ligation detection reaction
KW - PCR
KW - Polymerase chain reaction
KW - Universal array
UR - http://www.scopus.com/inward/record.url?scp=10744229040&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=10744229040&partnerID=8YFLogxK
M3 - Article
C2 - 14995096
AN - SCOPUS:10744229040
VL - 52
SP - 1049
EP - 1054
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 5
ER -