Detection and recovery of circulating colon cancer cells using a dielectrophoresis-based device: KRAS mutation status in pure CTCs

Francesco Fabbri, Silvia Carloni, Wainer Zoli, Paola Ulivi, Giulia Gallerani, Pietro Fici, Elisa Chiadini, Alessandro Passardi, Giovanni L. Frassineti, Angela Ragazzini, Dino Amadori

Research output: Contribution to journalArticle

Abstract

The characterization of circulating tumor cells (CTCs) could substantially improve the management of cancer patients. However, their study is still a matter of debate, often due to lymphocyte contamination. In the present paper, an investigation of CTCs was carried out for the first time using DEPArray, a dielectrophoresis-based platform able to detect and sort pure CTCs. Analyses were conducted on peripheral blood (PB) samples from patients with metastatic colon cancer. After 100% pure cell recovery and whole genome amplification, KRAS gene mutation of CTCs was screened and compared to gene status in the primary tumor tissue. CTCs were found in 21 colon cancer patients (52.5%), with more than three tumor cells per 7.5. ml. KRAS gene mutation analysis, showed a mutational concordance between CTCs and primary tumor in 50% of matched cases. The present study demonstrates for the first time the feasibility of analyzing at the molecular level pure CTCs avoiding lymphocyte contamination using an innovative instrumentation, and a KRAS discordance between CTCs and primary tissue. Our results present dielectrophoresis-based procedures as a new standard in single cell analysis and recovery and invite careful reflection on the value of CTCs characterization.

Original languageEnglish
Pages (from-to)225-231
Number of pages7
JournalCancer Letters
Volume335
Issue number1
DOIs
Publication statusPublished - Jul 10 2013

Keywords

  • Colorectal cancer
  • CTCs
  • Dielectrophoresis
  • Heterogeneity
  • KRAS

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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