Abstract
Capillary electrophoresis (CE) was used to characterize restriction fragment length polymorphism (RFLP) in a polymerase chain reaction (PCR)-amplified product of a 740-base pairs DNA fragment from the DXS 164 locus of the dystrophin gene. The polymorphic alleles of 740 and 520/220 base pairs revealed by XmnI digestion were analysed from homozygous and heterozygous individuals by CE. Our studies show that extraction in phenol-chloroform may be useful in PCR-amplified product purification. Excellent separation was obtained in a short time. The data indicate that CE is suitable for genomic analysis such as carrier detection and prenatal diagnosis of X-linked recessive disorders after purification of PCR-amplified products.
Original language | English |
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Pages (from-to) | 277-281 |
Number of pages | 5 |
Journal | Journal of Chromatography |
Volume | 638 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1993 |
ASJC Scopus subject areas
- Clinical Biochemistry
- Molecular Medicine