Detection by capillary electrophoresis of restriction fragment length polymorphism. Analysis of a polymerase chain reaction-amplified product of the DXS 164 locus in the dystrophin gene

D. Del Principe, M. P. Iampieri, D. Germani, A. Menichelli, G. Novelli, B. Dallapiccola

Research output: Contribution to journalArticle

Abstract

Capillary electrophoresis (CE) was used to characterize restriction fragment length polymorphism (RFLP) in a polymerase chain reaction (PCR)-amplified product of a 740-base pairs DNA fragment from the DXS 164 locus of the dystrophin gene. The polymorphic alleles of 740 and 520/220 base pairs revealed by XmnI digestion were analysed from homozygous and heterozygous individuals by CE. Our studies show that extraction in phenol-chloroform may be useful in PCR-amplified product purification. Excellent separation was obtained in a short time. The data indicate that CE is suitable for genomic analysis such as carrier detection and prenatal diagnosis of X-linked recessive disorders after purification of PCR-amplified products.

Original languageEnglish
Pages (from-to)277-281
Number of pages5
JournalJournal of Chromatography
Volume638
Issue number2
DOIs
Publication statusPublished - 1993

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Medicine

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