Detection of a 5′ UTR variation in the HCV genome after long-term in vitro infection

M. B. Valli, L. Bertolini, S. Iacovacci, A. Ponzetto, G. Carloni

Research output: Contribution to journalArticlepeer-review


The TOFE lymphoid cell line from normal human bone marrow is susceptible to infection by a hepatitis C virus (HCV) serum strain. A sequence analysis of the 5′ untranslated region (UTR) of HCV before and after long-term in vitro infection revealed one base substitution at position -158 (C > T) of the 5′ UTR. We performed the direct sequencing of 5′ UTR polymerase chain reaction-amplified sequences of the HCV genome: a) from the original serum-derived strain; b) from TOFE cell extracts 6 months post infection. This base substitution in the regulatory elements of the 5′ UTR might be related to the ability of the virus to grow in cell culture.

Original languageEnglish
Pages (from-to)285-288
Number of pages4
JournalResearch in Virology
Issue number4
Publication statusPublished - 1995


  • 5′ UTR
  • Base substitution
  • HCV
  • Long-term in vitro infection
  • Replication
  • TOFE cells

ASJC Scopus subject areas

  • Immunology
  • Virology


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