Detection of a fluorescent-labeled avidin-nucleic acid nanoassembly by confocal laser endomicroscopy in the microvasculature of chronically inflamed intestinal mucosa

Andrea Buda, Sonia Facchin, Elisa Dassie, Elisabetta Casarin, Mark A. Jepson, Helmut Neumann, Giorgia Hatem, Stefano Realdon, Renata D’Incà, Giacomo Carlo Sturniolo, Margherita Morpurgo

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Inflammatory bowel diseases are chronic gastrointestinal pathologies causing great discomfort in both children and adults. The pathogenesis of inflammatory bowel diseases is not yet fully understood and their diagnosis and treatment are often challenging. Nanoparticle-based strategies have been tested in local drug delivery to the inflamed colon. Here, we have investi­gated the use of the novel avidin-nucleic acid nanoassembly (ANANAS) platform as a potential diagnostic carrier in an experimental model of inflammatory bowel diseases. Fluorescent-labeled ANANAS nanoparticles were administered to mice with chemically induced chronic inflammation of the large intestine. Localization of mucosal nanoparticles was assessed in vivo by dual-band confocal laser endomicroscopy. This technique enables characterization of the mucosal microvasculature and crypt architecture at subcellular resolution. Intravascular nanoparticle distribution was observed in the inflamed mucosa but not in healthy controls, demonstrating the utility of the combination of ANANAS and confocal laser endomicroscopy for highlighting intestinal inflammatory conditions. The specific localization of ANANAS in inflamed tissues supports the potential of this platform as a targeted carrier for bioactive moieties in the treatment of inflammatory bowel disease.

Original languageEnglish
Pages (from-to)399-408
Number of pages10
JournalInternational Journal of Nanomedicine
Volume10
DOIs
Publication statusPublished - Jan 8 2015

Fingerprint

Avidin
Nucleic acids
Intestinal Mucosa
Microvessels
Inflammatory Bowel Diseases
Nanoparticles
Nucleic Acids
Lasers
Large Intestine
Pathology
Drug delivery
Colon
Mucous Membrane
Theoretical Models
Tissue
Inflammation
Therapeutics
Pharmaceutical Preparations

Keywords

  • Avidin-nucleic acid nanoassembly
  • Confocal laser endomicroscopy
  • Dextran sodium sulfate
  • Diagnostics
  • Fluorescent nanoparticles
  • Inflammatory bowel disease
  • Ulcerative colitis

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Organic Chemistry
  • Drug Discovery

Cite this

Detection of a fluorescent-labeled avidin-nucleic acid nanoassembly by confocal laser endomicroscopy in the microvasculature of chronically inflamed intestinal mucosa. / Buda, Andrea; Facchin, Sonia; Dassie, Elisa; Casarin, Elisabetta; Jepson, Mark A.; Neumann, Helmut; Hatem, Giorgia; Realdon, Stefano; D’Incà, Renata; Sturniolo, Giacomo Carlo; Morpurgo, Margherita.

In: International Journal of Nanomedicine, Vol. 10, 08.01.2015, p. 399-408.

Research output: Contribution to journalArticle

Buda, Andrea ; Facchin, Sonia ; Dassie, Elisa ; Casarin, Elisabetta ; Jepson, Mark A. ; Neumann, Helmut ; Hatem, Giorgia ; Realdon, Stefano ; D’Incà, Renata ; Sturniolo, Giacomo Carlo ; Morpurgo, Margherita. / Detection of a fluorescent-labeled avidin-nucleic acid nanoassembly by confocal laser endomicroscopy in the microvasculature of chronically inflamed intestinal mucosa. In: International Journal of Nanomedicine. 2015 ; Vol. 10. pp. 399-408.
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