Detection of a fluorescent-labeled avidin-nucleic acid nanoassembly by confocal laser endomicroscopy in the microvasculature of chronically inflamed intestinal mucosa

Andrea Buda, Sonia Facchin, Elisa Dassie, Elisabetta Casarin, Mark A. Jepson, Helmut Neumann, Giorgia Hatem, Stefano Realdon, Renata D’Incà, Giacomo Carlo Sturniolo, Margherita Morpurgo

Research output: Contribution to journalArticlepeer-review

Abstract

Inflammatory bowel diseases are chronic gastrointestinal pathologies causing great discomfort in both children and adults. The pathogenesis of inflammatory bowel diseases is not yet fully understood and their diagnosis and treatment are often challenging. Nanoparticle-based strategies have been tested in local drug delivery to the inflamed colon. Here, we have investi­gated the use of the novel avidin-nucleic acid nanoassembly (ANANAS) platform as a potential diagnostic carrier in an experimental model of inflammatory bowel diseases. Fluorescent-labeled ANANAS nanoparticles were administered to mice with chemically induced chronic inflammation of the large intestine. Localization of mucosal nanoparticles was assessed in vivo by dual-band confocal laser endomicroscopy. This technique enables characterization of the mucosal microvasculature and crypt architecture at subcellular resolution. Intravascular nanoparticle distribution was observed in the inflamed mucosa but not in healthy controls, demonstrating the utility of the combination of ANANAS and confocal laser endomicroscopy for highlighting intestinal inflammatory conditions. The specific localization of ANANAS in inflamed tissues supports the potential of this platform as a targeted carrier for bioactive moieties in the treatment of inflammatory bowel disease.

Original languageEnglish
Pages (from-to)399-408
Number of pages10
JournalInternational Journal of Nanomedicine
Volume10
DOIs
Publication statusPublished - Jan 8 2015

Keywords

  • Avidin-nucleic acid nanoassembly
  • Confocal laser endomicroscopy
  • Dextran sodium sulfate
  • Diagnostics
  • Fluorescent nanoparticles
  • Inflammatory bowel disease
  • Ulcerative colitis

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Organic Chemistry
  • Drug Discovery

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